ABSTRACT OBJECTIVE: As the peroxisome proliferator-activated receptor _γ_ (PPAR_γ_) plays a central role in fat mass regulation, we investigated whether initial subcutaneous PPAR_γ_ activity

is related to fat mass generation during overfeeding. SUBJECTS: Fourteen healthy female subjects (age 25±4 years, BMI 22.1±2.3 kg/m2). DESIGN AND MEASUREMENTS: Subjects were overfed with a

diet supplying 50% more energy than baseline energy requirements for 14 days. Fasting blood samples were analyzed for leptin, insulin and glucose. Fasting subcutaneous abdominal fat biopsies

were obtained for analysis of PPAR_γ_1, PPAR_γ_2, aP2 and UCP2 mRNAs. RESULTS: Initial PPAR_γ_1 and 2, aP2 and UCP2 mRNAs were not related to fat gain (_P_>0.12). However, PPAR_γ_1,

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ACKNOWLEDGEMENTS We thank Erwin Thimister and Chris Hukshorn for obtaining blood and fat samples and Jolanda van der Meijde and Esther Moonen-Kornips for mRNA measurements. AUTHOR

INFORMATION AUTHORS AND AFFILIATIONS * Department of Human Biology, Maastricht University, Maastricht, The Netherlands A M C P Joosen, A H F Bakker, A H G Zorenc, P Schrauwen & K R

Westerterp * Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands S Kersten Authors * A M C P Joosen View author publications You can also search for this author

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M C P Joosen. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Joosen, A., Bakker, A., Zorenc, A. _et al._ PPAR_γ_ activity in subcutaneous abdominal fat

tissue and fat mass gain during short-term overfeeding. _Int J Obes_ 30, 302–307 (2006). https://doi.org/10.1038/sj.ijo.0803146 Download citation * Received: 23 March 2005 * Revised: 30

August 2005 * Accepted: 16 September 2005 * Published: 25 October 2005 * Issue Date: 01 February 2006 * DOI: https://doi.org/10.1038/sj.ijo.0803146 SHARE THIS ARTICLE Anyone you share the

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Nature SharedIt content-sharing initiative KEYWORDS * peroxisome proliferator-activated receptor _γ_ * uncoupling protein 2 * adipogenesis * lipogenesis * humans