Cloning and characterization of human and mouse telomerase rna gene promoter sequences

Cloning and characterization of human and mouse telomerase rna gene promoter sequences

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ABSTRACT Variation in telomerase activity is correlated with cellular senescence and tumour progression. However, although the enzymatic activity of telomerase has been well studied, very little is known about how expression of telomerase genes is regulated in mammalian cells. We have therefore cloned the promoter regions of the human (hTR), and mouse, (_TERC_), telomerase RNA genes in order to identify the regulatory elements controlling telomerase RNA gene transcription. 1.76 kb encompassing the hTR gene promoter region was sequenced, as was 4 kb encompassing the _TERC_ promoter. No significant sequence similarity could be detected in comparisons between human and mouse 5′-regions, flanking the transcribed sequences. However, both the human and mouse telomerase RNA genes are within CpG islands and may therefore be under the regulation of DNA methylation. Transient expression of hTR-reporter gene constructs in HeLa and GM847 cells identified the elements responsible for promoter activity are contained in a 231 bp region upstream of the transcriptional start site. Transient expression of _TERC_-reporter gene constructs in Swiss3T3 and A9 cells identified the elements responsible for promoter activity are contained in a 73 bp region upstream of the transcriptional start site. These studies have implications for novel transcription targeted cancer therapies. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 50 print issues and online access $259.00 per year only $5.18 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS MODIFICATION OF THE TELOMERASE GENE WITH HUMAN REGULATORY SEQUENCES RESETS MOUSE TELOMERES TO HUMAN LENGTH Article Open access 04 February 2025 TERT PROMOTER ALTERATIONS COULD PROVIDE A SOLUTION FOR PETO’S PARADOX IN RODENTS Article Open access 30 November 2020 TELOMERES REFORGED WITH NON-TELOMERIC SEQUENCES IN MOUSE EMBRYONIC STEM CELLS Article Open access 17 February 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * CRC Department of Medical Oncology, University of Glasgow, CRC Beatson Laboratories, Alexander Stone Building, Garscube Estate, Switchback Road, Bearsden, G61 1BD, Glasgow Jiang-Qin Zhao, Stacey F Hoare, Sharon Muir & W Nicol Keith * Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, G61 1BD, Glasgow, UK Robert McFarlane, E Kenneth Parkinson & Donald M Black Authors * Jiang-Qin Zhao View author publications You can also search for this author inPubMed Google Scholar * Stacey F Hoare View author publications You can also search for this author inPubMed Google Scholar * Robert McFarlane View author publications You can also search for this author inPubMed Google Scholar * Sharon Muir View author publications You can also search for this author inPubMed Google Scholar * E Kenneth Parkinson View author publications You can also search for this author inPubMed Google Scholar * Donald M Black View author publications You can also search for this author inPubMed Google Scholar * W Nicol Keith View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Zhao, JQ., Hoare, S., McFarlane, R. _et al._ Cloning and characterization of human and mouse telomerase RNA gene promoter sequences. _Oncogene_ 16, 1345–1350 (1998). https://doi.org/10.1038/sj.onc.1201892 Download citation * Received: 17 December 1997 * Revised: 03 February 1998 * Accepted: 05 February 1998 * Published: 19 March 1998 * Issue Date: 12 March 1998 * DOI: https://doi.org/10.1038/sj.onc.1201892 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * telomerase * gene expression * hTR * _terc_ * gene promoter * gene transcription

ABSTRACT Variation in telomerase activity is correlated with cellular senescence and tumour progression. However, although the enzymatic activity of telomerase has been well studied, very


little is known about how expression of telomerase genes is regulated in mammalian cells. We have therefore cloned the promoter regions of the human (hTR), and mouse, (_TERC_), telomerase


RNA genes in order to identify the regulatory elements controlling telomerase RNA gene transcription. 1.76 kb encompassing the hTR gene promoter region was sequenced, as was 4 kb


encompassing the _TERC_ promoter. No significant sequence similarity could be detected in comparisons between human and mouse 5′-regions, flanking the transcribed sequences. However, both


the human and mouse telomerase RNA genes are within CpG islands and may therefore be under the regulation of DNA methylation. Transient expression of hTR-reporter gene constructs in HeLa and


GM847 cells identified the elements responsible for promoter activity are contained in a 231 bp region upstream of the transcriptional start site. Transient expression of _TERC_-reporter


gene constructs in Swiss3T3 and A9 cells identified the elements responsible for promoter activity are contained in a 73 bp region upstream of the transcriptional start site. These studies


have implications for novel transcription targeted cancer therapies. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution


ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 50 print issues and online access $259.00 per year only $5.18 per issue Learn more Buy this article *


Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn


about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS MODIFICATION OF THE TELOMERASE GENE WITH HUMAN REGULATORY SEQUENCES RESETS


MOUSE TELOMERES TO HUMAN LENGTH Article Open access 04 February 2025 TERT PROMOTER ALTERATIONS COULD PROVIDE A SOLUTION FOR PETO’S PARADOX IN RODENTS Article Open access 30 November 2020


TELOMERES REFORGED WITH NON-TELOMERIC SEQUENCES IN MOUSE EMBRYONIC STEM CELLS Article Open access 17 February 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * CRC Department of Medical


Oncology, University of Glasgow, CRC Beatson Laboratories, Alexander Stone Building, Garscube Estate, Switchback Road, Bearsden, G61 1BD, Glasgow Jiang-Qin Zhao, Stacey F Hoare, Sharon Muir 


& W Nicol Keith * Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, G61 1BD, Glasgow, UK Robert McFarlane, E Kenneth Parkinson & 


Donald M Black Authors * Jiang-Qin Zhao View author publications You can also search for this author inPubMed Google Scholar * Stacey F Hoare View author publications You can also search for


this author inPubMed Google Scholar * Robert McFarlane View author publications You can also search for this author inPubMed Google Scholar * Sharon Muir View author publications You can


also search for this author inPubMed Google Scholar * E Kenneth Parkinson View author publications You can also search for this author inPubMed Google Scholar * Donald M Black View author


publications You can also search for this author inPubMed Google Scholar * W Nicol Keith View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND


PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Zhao, JQ., Hoare, S., McFarlane, R. _et al._ Cloning and characterization of human and mouse telomerase RNA gene


promoter sequences. _Oncogene_ 16, 1345–1350 (1998). https://doi.org/10.1038/sj.onc.1201892 Download citation * Received: 17 December 1997 * Revised: 03 February 1998 * Accepted: 05 February


1998 * Published: 19 March 1998 * Issue Date: 12 March 1998 * DOI: https://doi.org/10.1038/sj.onc.1201892 SHARE THIS ARTICLE Anyone you share the following link with will be able to read


this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative


KEYWORDS * telomerase * gene expression * hTR * _terc_ * gene promoter * gene transcription