ABSTRACT Immunohistochemical analysis of paired tumor and normal tissue specimens revealed that the expression and cytoplasmic abundance of the RNA-binding protein HuR increased with

malignancy, particularly in colon carcinomas. Interventions to modulate HuR expression in human RKO colon cancer cells altered gene expression profiles and identified _β_-catenin mRNA as a

novel HuR target. Subcutaneous injection of HuR-overexpressing RKO cells into nude mice produced significantly larger tumors than those arising from control populations; conversely, RKO

cells expressing reduced HuR through small interference RNA- or _antisense_ HuR-based approaches developed significantly more slowly. We propose that HuR-regulated target mRNA expression

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and Gorospe M . (2001). _Mol. Cell. Biol._, 21, 5889–5898. * Wong NA and Pignatelli M . (2002). _Am. J. Pathol._, 160, 389–401. Download references ACKNOWLEDGEMENTS We thank MB Kastan for

the RKO cells, S Galbán, M Jr Ware, J Robinson, C Galbán, and J Alsruhe for assistance with experimental procedures, and KG Becker and C Cheadle for providing cDNA arrays and guidance in

their analysis. We are grateful to our colleagues PJ Morin and DL Longo for critical reading of the manuscript. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Laboratory of Cellular and

Molecular Biology, Baltimore, 21224, MD, USA Isabel López de Silanes, Jinshui Fan, Xiaoling Yang & Myriam Gorospe * Research Resources Branch, National Institute on Aging-Intramural

Research Program, National Institutes of Health, Baltimore, 21224, MD, USA Alan B Zonderman * Preclinical Research and Translational Medicine, SUGEN, Inc., San Francisco, 94080, CA, USA Olga

Potapova * Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, 21224, MD, USA Ellen S Pizer Authors * Isabel López de Silanes View author publications You can also

search for this author inPubMed Google Scholar * Jinshui Fan View author publications You can also search for this author inPubMed Google Scholar * Xiaoling Yang View author publications You

can also search for this author inPubMed Google Scholar * Alan B Zonderman View author publications You can also search for this author inPubMed Google Scholar * Olga Potapova View author

publications You can also search for this author inPubMed Google Scholar * Ellen S Pizer View author publications You can also search for this author inPubMed Google Scholar * Myriam Gorospe

View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Myriam Gorospe. RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE de Silanes, I., Fan, J., Yang, X. _et al._ Role of the RNA-binding protein HuR in colon carcinogenesis. _Oncogene_ 22, 7146–7154 (2003).

https://doi.org/10.1038/sj.onc.1206862 Download citation * Received: 25 February 2003 * Revised: 11 May 2003 * Accepted: 04 June 2003 * Published: 16 October 2003 * Issue Date: 16 October

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is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * HuR * ELAV * colon cancer * mRNA turnover *

carcinogenesis