ABSTRACT To identify genes that are differentially expressed in human esophageal squamous cell carcinoma (ESCC), we have developed a cDNA microarray representing 34 176 clones to analyse

gene expression profiles in ESCC. A total of 77 genes (including 31 novel genes) were downregulated, and 15 genes (including one novel gene) were upregulated in cancer tissues compared with

their normal counterparts. Immunohistochemistry and Northern blot analysis were carried out to verify the cDNA microarray results. It was revealed that genes involved in squamous cell

differentiation were coordinately downregulated, including annexin I, small proline-rich proteins (SPRRs), calcium-binding S100 proteins (S100A8, S100A9), transglutaminase (TGM3),

cystein (SPARC), cathepsin B and KRT17. Functional analysis of the alteration in the expression of GKLF suggested that GKLF might be able to regulate the expression of SPRR1A, SPRR2A and

KRT4 in ESCC. This study provides new insights into the role of squamous cell differentiation-associated genes in ESCC initiation and progression. Access through your institution Buy or

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SCREENING AND VERIFICATION OF HUB GENES IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA BY INTEGRATED ANALYSIS Article Open access 22 March 2024 IDENTIFICATION OF CANDIDATE ABERRANTLY METHYLATED AND

DIFFERENTIALLY EXPRESSED GENES IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA Article Open access 16 June 2020 DISSECTING ESOPHAGEAL SQUAMOUS-CELL CARCINOMA ECOSYSTEM BY SINGLE-CELL TRANSCRIPTOMIC

ANALYSIS Article Open access 06 September 2021 ABBREVIATIONS * C1orf10: chromosome 1 open reading frame 10 * ECM: extracellular matrix * EEF1A1: eukaryotic translation elongation factor 1

alpha 1 * EMP1: epithelial membrane protein 1 * ESCC: esophageal squamous cell carcinoma * ESPC: esophageal cancer cDNA library * ESPN: normal esophagus cDNA library * EST: expressed

sequence tag * GKLF: gut-enriched Kruppel-like factor * KRT: cytokeratin * PKD1: polycystic kidney disease 1 * RHCG: Rh type C glycoprotein * S100: S100 calcium-binding protein * SPARC:

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study was cosupported by China Key Program on Basic Research (G1998051021), the Chinese Hi-tech R&D program (2001AA231041), National Science Foundation of China (30170519) and China

National Center Biotechnology Development. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * National Lab of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences &

Peking Union Medical College, Beijing, 100021, P.R., China Aiping Luo, Jianping Kong, Xiuqin Wang, Junfang Ji, Tao Wang, Huiying Zhi, Min Wu & Zhihua Liu * Shanghai Institute of

Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, P.R., China Gengxi Hu * Brigham and Women's Hospital, Harvard Medical School, 75 Francis

St Thorn 1334, Boston, 02115, USA Choong-Chin Liew * Human Genetics Center, University of Texas, Health Science Center at Houston, Houston, 77030, TX, USA Momiao Xiong Authors * Aiping Luo

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RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Luo, A., Kong, J., Hu, G. _et al._ Discovery of Ca2+-relevant and differentiation-associated genes

downregulated in esophageal squamous cell carcinoma using cDNA microarray. _Oncogene_ 23, 1291–1299 (2004). https://doi.org/10.1038/sj.onc.1207218 Download citation * Received: 04 June 2003

* Revised: 16 September 2003 * Accepted: 17 September 2003 * Published: 01 December 2003 * Issue Date: 12 February 2004 * DOI: https://doi.org/10.1038/sj.onc.1207218 SHARE THIS ARTICLE

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by the Springer Nature SharedIt content-sharing initiative KEYWORDS * differentiation * calcium * cell envelope * esophageal squamous cell carcinoma * cDNA microarray