ABSTRACT For the better understanding of engraftment properties after autologous peripheral blood stem cell transplantation (PBSCT), hematopoietic recovery, immune reconstitution and

functional capacity of cytokine production in different lymphocyte populations were examined. In a prospective study, we examined 24 patients suffering from different malignancies after

autologous PBSCT. The examination intervals were 1, 3, 6 and 12 months after PBSCT. T cells, B cells and NK cells were analyzed using flow cytometry. The expression and kinetics of cytokines

in T lymphocytes were evaluated in 10 patients by intracellular staining of cytokines after PMA/ionomycin stimulation. We observed rapid hematopoietic engraftment proceeding to stable

cytokine-producing T cells and the mean fluorescence intensity (MFI) shifts suggested an insufficient capacity for producing IFNγ, in particular for CD3+CD4+ T cells, compared to healthy

volunteers early after PBSCT. Rapid hematopoietic recovery and partly impaired immune reconstitution, especially regarding the regeneration of B lymphocytes and T helper cells, was observed.

The CD4+ subpopulation remained low throughout the period of examination, whereas the B cells showed a delayed recovery after 3 months. Cytokine production proved to be sufficient after _in

vitro_ stimulation in T cell populations with the exception of IFNγ synthesis. _Bone Marrow Transplantation_ (2001) 28, 251–257. Access through your institution Buy or subscribe This is a

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Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institute for Immunology and Transfusion Medicine, University of Lübeck School of Medicine, Germany P Schlenke, S

Sheikhzadeh & H Kirchner * Department of Internal Medicine, Section Hematology and Oncology, University of Lübeck School of Medicine, Germany K Weber & T Wagner Authors * P Schlenke

View author publications You can also search for this author inPubMed Google Scholar * S Sheikhzadeh View author publications You can also search for this author inPubMed Google Scholar * K

Weber View author publications You can also search for this author inPubMed Google Scholar * T Wagner View author publications You can also search for this author inPubMed Google Scholar * H

Kirchner View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Schlenke,

P., Sheikhzadeh, S., Weber, K. _et al._ Immune reconstitution and production of intracellular cytokines in T lymphocyte populations following autologous peripheral blood stem cell

transplantation. _Bone Marrow Transplant_ 28, 251–257 (2001). https://doi.org/10.1038/sj.bmt.1703121 Download citation * Received: 21 December 2000 * Accepted: 15 May 2001 * Published: 04

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chemotherapy * autologous stem cell transplantation * CD34+ cells * immune reconstitution * lymphocyte subsets * intracellular cytokines