SINCE the introduction of solid medium, the isolation of micro-organisms in pure culture, formerly a matter of considerable difficulty, has become a relatively easy process. The numerous

studies of micro-organisms in pure culture which resulted have enabled the science of microbiology to develop rapidly. In Nature, however, micro-organisms are rarely found in pure culture,

and there is a need for investigation of the growth and metabolism of organisms growing in association with each other. Woods1 has recently stressed the need for such research. The present

study of a yeast (Saccharomyces cerevisiae) and of a bacterium (a Lactobacillus species) growing separately, and also when growing in association with each other in defined media, was

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