ABSTRACT THE treatment of collagen with hydroxylamine, which splits ester-like bonds, produces subunits carrying newly formed aspartic acid hydroxamates1,2. Their alcohol counterparts are

not yet fully identified. A series of investigations on the immunogenicity and specificity of collagen3,4 showed that collagen treated with hydroxylamine (HC) is an antigen which induces the

formation of two types of immune sera: type 1 containing only antibodies against the area of hydroxylamine treatment (HC-antibodies); type 2 containing additionally antibodies against the

general collagen structure. HC therefore acts when used for immunization like a conjugated protein. Investigation of haemagglutination inhibition showed that early sera were predominantly of

Additionally, inhibition experiments were carried out with peptides obtained from HC, acid-soluble collagen and insoluble collagen by treatment with trypsin or colla-genase5. Only peptides

obtained from HC inhibited HC-antibodies and these peptides when used in sufficient amounts caused a complete inhibition of potent antisera. Access through your institution Buy or subscribe

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FRUCTOSYLATED PEPTIDE DERIVED FROM A COLLAGEN II T CELL EPITOPE FOR LONG-TERM TREATMENT OF ARTHRITIS (FIA-CIA) IN MICE Article Open access 30 August 2021 SERUM COMPONENTS INFLUENCE ANTIBODY

REACTIVITY TO GLYCAN AND DNA ANTIGENS Article Open access 22 August 2023 REGULATORY PROPERTIES OF VITRONECTIN AND ITS GLYCOSYLATION IN COLLAGEN FIBRIL FORMATION AND COLLAGEN-DEGRADING ENZYME

CATHEPSIN K ACTIVITY Article Open access 08 June 2021 REFERENCES * Gallop, P. M., Seifter, S., and Meilman, E., _Nature_, 183, 1659 (1959). Article  ADS  CAS  Google Scholar  * Blumenfeld,

O. O., and Gallop, P. M., _Biochemistry_, 1, 947 (1962). Article  CAS  Google Scholar  * Steffen, C., Timpl, R., and Wolff, I., _Z. Immunitätsforsch._, 124, 476 (1962). Google Scholar  *

Steffen, C., Timpl, R., and Wolff, I., _J. Immunol._, 93, 656 (1964). CAS  PubMed  Google Scholar  * Steffen, C., and Timpl, R., _Z. Immunitätsforsch._, 130, 3 (1966). CAS  Google Scholar  *

Springer, G. F., and Williamson, P., _Biochem. J._, 85, 282 (1962). Article  CAS  Google Scholar  * Hörmann, H., _Leder_, 11, 173 (1960). Google Scholar  * Porter, R. R., and Press, E. M.,

_Ann. Rev. Biochem._, 31, 625 (1962). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Klinisches Laboratorium, Hanuschkrankenhaus, Vienna,

Austria C. STEFFEN, R. TIMPL & I. WOLFF Authors * C. STEFFEN View author publications You can also search for this author inPubMed Google Scholar * R. TIMPL View author publications You

can also search for this author inPubMed Google Scholar * I. WOLFF View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE STEFFEN, C., TIMPL, R. & WOLFF, I. Identification of an Antigenic Determinant of Collagen treated with Hydroxylamine. _Nature_ 213,

497–498 (1967). https://doi.org/10.1038/213497a0 Download citation * Issue Date: 04 February 1967 * DOI: https://doi.org/10.1038/213497a0 SHARE THIS ARTICLE Anyone you share the following

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