Rubella haemagglutinin prepared in bs-c-1 cells

Rubella haemagglutinin prepared in bs-c-1 cells

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ABSTRACT STEWART _et al._1 described procedures for the preparation of rubella virus haemagglutinin in monolayers of BHK21 cells. Others expanded these studies to include extraction of haemagglutinin with high _p_H buffers from BHK21 suspension cultures infected with rubella virus2. Production of rubella virus haemagglutinin in an established line of African green monkey kidney cells (VERO) was recently reported3. We describe the production of haemagglutinin in the BS-C-1 line of African green monkey cells by two different strains of rubella virus and subsequent establishment of a chronically infected culture which continues to shed virus and haemagglutinin after serial passage. SIMILAR CONTENT BEING VIEWED BY OTHERS A HAEMAGGLUTINATION TEST FOR RAPID DETECTION OF ANTIBODIES TO SARS-COV-2 Article Open access 29 March 2021 BROADLY NEUTRALIZING ANTI-HIV-1 ANTIBODIES TETHER VIRAL PARTICLES AT THE SURFACE OF INFECTED CELLS Article Open access 02 February 2022 RHESUS CYTOMEGALOVIRUS-ENCODED FCΓ-BINDING GLYCOPROTEINS FACILITATE VIRAL EVASION FROM IGG-MEDIATED HUMORAL IMMUNITY Article Open access 31 January 2025 ARTICLE PDF REFERENCES * Stewart, G. L., Parkman, P. D., Hopps, H. E., Douglas, R. D., Hamilton, J. P., and Meyer, jun., H. M., _N. Engl. J. Med._, 276, 554 (1967). Article  CAS  Google Scholar  * Halonen, P. E., Ryan, J. M., and Stewart, J. A., _Proc. Soc. Exp. Biol. and Med._, 125, 162 (1967). Article  CAS  Google Scholar  * Rafajko, R. R., and zur Nedden, D. L., _Appl. Microbiol._, 16, 803 (1968). PubMed  PubMed Central  CAS  Google Scholar  * Parkman, P. D., Buescher, E. L., and Artenstein, M. S., _Proc. Soc. Exp. Biol. and Med._, 111, 225 (1962). Article  CAS  Google Scholar  * Rafajko, R. R., Polakavetz, S., Handelman, B., and zur Nedden, D. L., _Appl. Microbiol._, 16, 423 (1968). PubMed  PubMed Central  CAS  Google Scholar  * Massab, H. F., and Veronelli, J. A., _J. Bact._, 91, 436 (1966). Google Scholar  * Plotkin, S. A., _Amer. J. Med. Sci._, 253, 132/356 (1967). Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Medical Research Consultants, 12601 Twinbrook Parkway, Rockville, Maryland ROBERT R. RAFAJKO & DOBOTHY ZUR NEDDEN Authors * ROBERT R. RAFAJKO View author publications You can also search for this author inPubMed Google Scholar * DOBOTHY ZUR NEDDEN View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE RAFAJKO, R., ZUR NEDDEN, D. Rubella Haemagglutinin prepared in BS-C-1 Cells. _Nature_ 220, 1347–1349 (1968). https://doi.org/10.1038/2201347a0 Download citation * Received: 07 October 1968 * Issue Date: 28 December 1968 * DOI: https://doi.org/10.1038/2201347a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT STEWART _et al._1 described procedures for the preparation of rubella virus haemagglutinin in monolayers of BHK21 cells. Others expanded these studies to include extraction of


haemagglutinin with high _p_H buffers from BHK21 suspension cultures infected with rubella virus2. Production of rubella virus haemagglutinin in an established line of African green monkey


kidney cells (VERO) was recently reported3. We describe the production of haemagglutinin in the BS-C-1 line of African green monkey cells by two different strains of rubella virus and


subsequent establishment of a chronically infected culture which continues to shed virus and haemagglutinin after serial passage. SIMILAR CONTENT BEING VIEWED BY OTHERS A HAEMAGGLUTINATION


TEST FOR RAPID DETECTION OF ANTIBODIES TO SARS-COV-2 Article Open access 29 March 2021 BROADLY NEUTRALIZING ANTI-HIV-1 ANTIBODIES TETHER VIRAL PARTICLES AT THE SURFACE OF INFECTED CELLS


Article Open access 02 February 2022 RHESUS CYTOMEGALOVIRUS-ENCODED FCΓ-BINDING GLYCOPROTEINS FACILITATE VIRAL EVASION FROM IGG-MEDIATED HUMORAL IMMUNITY Article Open access 31 January 2025


ARTICLE PDF REFERENCES * Stewart, G. L., Parkman, P. D., Hopps, H. E., Douglas, R. D., Hamilton, J. P., and Meyer, jun., H. M., _N. Engl. J. Med._, 276, 554 (1967). Article  CAS  Google


Scholar  * Halonen, P. E., Ryan, J. M., and Stewart, J. A., _Proc. Soc. Exp. Biol. and Med._, 125, 162 (1967). Article  CAS  Google Scholar  * Rafajko, R. R., and zur Nedden, D. L., _Appl.


Microbiol._, 16, 803 (1968). PubMed  PubMed Central  CAS  Google Scholar  * Parkman, P. D., Buescher, E. L., and Artenstein, M. S., _Proc. Soc. Exp. Biol. and Med._, 111, 225 (1962). Article


  CAS  Google Scholar  * Rafajko, R. R., Polakavetz, S., Handelman, B., and zur Nedden, D. L., _Appl. Microbiol._, 16, 423 (1968). PubMed  PubMed Central  CAS  Google Scholar  * Massab, H.


F., and Veronelli, J. A., _J. Bact._, 91, 436 (1966). Google Scholar  * Plotkin, S. A., _Amer. J. Med. Sci._, 253, 132/356 (1967). Download references AUTHOR INFORMATION AUTHORS AND


AFFILIATIONS * Medical Research Consultants, 12601 Twinbrook Parkway, Rockville, Maryland ROBERT R. RAFAJKO & DOBOTHY ZUR NEDDEN Authors * ROBERT R. RAFAJKO View author publications You


can also search for this author inPubMed Google Scholar * DOBOTHY ZUR NEDDEN View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS


Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE RAFAJKO, R., ZUR NEDDEN, D. Rubella Haemagglutinin prepared in BS-C-1 Cells. _Nature_ 220, 1347–1349 (1968).


https://doi.org/10.1038/2201347a0 Download citation * Received: 07 October 1968 * Issue Date: 28 December 1968 * DOI: https://doi.org/10.1038/2201347a0 SHARE THIS ARTICLE Anyone you share


the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer


Nature SharedIt content-sharing initiative