Lac repressor can be fused to β-galactosidase

Lac repressor can be fused to β-galactosidase

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ABSTRACT GENETIC analysis of _i_ gene mutants which had lost the capacity to bind operator but not inducer suggested that _lac_ repressor binds with its amino terminus to _lac_ operator1. Here we will show that the carboxy terminus of _lac_ repressor is dispensable: We found fusions between the _i_ (_lac_ repressor) and _z_ (_β_-galactosidase) genes (Fig. 1) which produce fully active _lac_ repressor covalently linked to active _β_-galactosidase. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS GENERATION OF BRIGHT AUTOBIOLUMINESCENT BACTERIA BY CHROMOSOMAL INTEGRATION OF THE IMPROVED _LUX_ OPERON _ILUX2_ Article Open access 09 November 2022 ENGINEERED SYSTEMS OF INDUCIBLE ANTI-REPRESSORS FOR THE NEXT GENERATION OF BIOLOGICAL PROGRAMMING Article Open access 07 September 2020 OPTOGENETIC CONTROL OF THE _LAC_ OPERON FOR BACTERIAL CHEMICAL AND PROTEIN PRODUCTION Article 07 September 2020 REFERENCES * Adler, K., _et al._, _Nature_, 237, 322–327 (1972). Article  ADS  CAS  Google Scholar  * Müller-Hill, B., Crapo, L., and Gilbert, W., _Proc. natn. Acad. Sci U.S.A._, 59, 1259–1264 (1968). Article  ADS  Google Scholar  * Jacob, F., and Monod, J., _J. molec. Biol._, 3, 318–356 (1961). Article  CAS  Google Scholar  * Pfahl, M. L., _Genetics_, 72, 393–410 (1972). CAS  PubMed  PubMed Central  Google Scholar  * Zipser, D., Zabell, S., Rothman, J., Grodzicker, T., Wenk, M., and Novitski, M., _J. molec. Biol._, 49, 251–254 (1970). Article  CAS  Google Scholar  * Miller, J. H., Reznikoff, W. S., Silverstone, A. E., Ippen, K., Signer, E. R., and Beckwith, J. R., _J. Bact._, 104, 1273–1279 (1970). CAS  PubMed  Google Scholar  * Gilbert, W., and Müller-Hill, B., _Proc. natn. Acad. Sci. USA._, 56, 1891–1898 (1966). Article  ADS  CAS  Google Scholar  * Platt, T., in _Experiments in Molecular Genetics_ (edit. by Miller, J. H.), 403. (Cold Spring Harbor Laboratory, New York, 1972). Google Scholar  * Fowler, A. V., and Zabin, I., _J. biol. Chem._, 245, 5032–5041 (1970). CAS  PubMed  Google Scholar  * Beyreuther, K., Adler, K., Geisler, N., and Klemm, A., _Proc. natn. Acad. Sci. U.S.A._, 70, 3576–3580 (1973). Article  ADS  CAS  Google Scholar  * Weiner, A. M., Platt, T., and Weber, K., _J. biol. Chem._, 247, 3242–3251 (1972). CAS  Google Scholar  * Platt, T., Files, J. G., and Weber, K., _J. biol. Chem._, 248, 110–121 (1973). CAS  PubMed  Google Scholar  * Weber, K., and Osborn, M., _J. biol. Chem._, 244, 4406–4412 (1969). CAS  Google Scholar  * Riggs, A. D., Suzuki, H., and Bourgeois, S., _J. molec. Biol._, 48, 67–83 (1970). Article  CAS  Google Scholar  * Bray, G. A., _Analyt. Biochem._, 1, 279–285 (1960). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institut für Genetik der Universität zu Köln, 5 Köln 41, Weyertal, 121, West Germany B. MüLLER-HILL & J. KANIA Authors * B. MüLLER-HILL View author publications You can also search for this author inPubMed Google Scholar * J. KANIA View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE MüLLER-HILL, B., KANIA, J. _Lac_ repressor can be fused to β-galactosidase. _Nature_ 249, 561–563 (1974). https://doi.org/10.1038/249561a0 Download citation * Received: 27 December 1973 * Revised: 19 March 1974 * Issue Date: 07 June 1974 * DOI: https://doi.org/10.1038/249561a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT GENETIC analysis of _i_ gene mutants which had lost the capacity to bind operator but not inducer suggested that _lac_ repressor binds with its amino terminus to _lac_ operator1.


Here we will show that the carboxy terminus of _lac_ repressor is dispensable: We found fusions between the _i_ (_lac_ repressor) and _z_ (_β_-galactosidase) genes (Fig. 1) which produce


fully active _lac_ repressor covalently linked to active _β_-galactosidase. Access through your institution Buy or subscribe This is a preview of subscription content, access via your


institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this


article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in


* Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS GENERATION OF BRIGHT AUTOBIOLUMINESCENT BACTERIA BY CHROMOSOMAL


INTEGRATION OF THE IMPROVED _LUX_ OPERON _ILUX2_ Article Open access 09 November 2022 ENGINEERED SYSTEMS OF INDUCIBLE ANTI-REPRESSORS FOR THE NEXT GENERATION OF BIOLOGICAL PROGRAMMING


Article Open access 07 September 2020 OPTOGENETIC CONTROL OF THE _LAC_ OPERON FOR BACTERIAL CHEMICAL AND PROTEIN PRODUCTION Article 07 September 2020 REFERENCES * Adler, K., _et al._,


_Nature_, 237, 322–327 (1972). Article  ADS  CAS  Google Scholar  * Müller-Hill, B., Crapo, L., and Gilbert, W., _Proc. natn. Acad. Sci U.S.A._, 59, 1259–1264 (1968). Article  ADS  Google


Scholar  * Jacob, F., and Monod, J., _J. molec. Biol._, 3, 318–356 (1961). Article  CAS  Google Scholar  * Pfahl, M. L., _Genetics_, 72, 393–410 (1972). CAS  PubMed  PubMed Central  Google


Scholar  * Zipser, D., Zabell, S., Rothman, J., Grodzicker, T., Wenk, M., and Novitski, M., _J. molec. Biol._, 49, 251–254 (1970). Article  CAS  Google Scholar  * Miller, J. H., Reznikoff,


W. S., Silverstone, A. E., Ippen, K., Signer, E. R., and Beckwith, J. R., _J. Bact._, 104, 1273–1279 (1970). CAS  PubMed  Google Scholar  * Gilbert, W., and Müller-Hill, B., _Proc. natn.


Acad. Sci. USA._, 56, 1891–1898 (1966). Article  ADS  CAS  Google Scholar  * Platt, T., in _Experiments in Molecular Genetics_ (edit. by Miller, J. H.), 403. (Cold Spring Harbor Laboratory,


New York, 1972). Google Scholar  * Fowler, A. V., and Zabin, I., _J. biol. Chem._, 245, 5032–5041 (1970). CAS  PubMed  Google Scholar  * Beyreuther, K., Adler, K., Geisler, N., and Klemm,


A., _Proc. natn. Acad. Sci. U.S.A._, 70, 3576–3580 (1973). Article  ADS  CAS  Google Scholar  * Weiner, A. M., Platt, T., and Weber, K., _J. biol. Chem._, 247, 3242–3251 (1972). CAS  Google


Scholar  * Platt, T., Files, J. G., and Weber, K., _J. biol. Chem._, 248, 110–121 (1973). CAS  PubMed  Google Scholar  * Weber, K., and Osborn, M., _J. biol. Chem._, 244, 4406–4412 (1969).


CAS  Google Scholar  * Riggs, A. D., Suzuki, H., and Bourgeois, S., _J. molec. Biol._, 48, 67–83 (1970). Article  CAS  Google Scholar  * Bray, G. A., _Analyt. Biochem._, 1, 279–285 (1960).


Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institut für Genetik der Universität zu Köln, 5 Köln 41, Weyertal, 121, West Germany B.


MüLLER-HILL & J. KANIA Authors * B. MüLLER-HILL View author publications You can also search for this author inPubMed Google Scholar * J. KANIA View author publications You can also


search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE MüLLER-HILL, B., KANIA, J. _Lac_ repressor can be fused to


β-galactosidase. _Nature_ 249, 561–563 (1974). https://doi.org/10.1038/249561a0 Download citation * Received: 27 December 1973 * Revised: 19 March 1974 * Issue Date: 07 June 1974 * DOI:


https://doi.org/10.1038/249561a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently


available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative