ABSTRACT GENETIC analysis of _i_ gene mutants which had lost the capacity to bind operator but not inducer suggested that _lac_ repressor binds with its amino terminus to _lac_ operator1.

Here we will show that the carboxy terminus of _lac_ repressor is dispensable: We found fusions between the _i_ (_lac_ repressor) and _z_ (_β_-galactosidase) genes (Fig. 1) which produce

fully active _lac_ repressor covalently linked to active _β_-galactosidase. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

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INTEGRATION OF THE IMPROVED _LUX_ OPERON _ILUX2_ Article Open access 09 November 2022 ENGINEERED SYSTEMS OF INDUCIBLE ANTI-REPRESSORS FOR THE NEXT GENERATION OF BIOLOGICAL PROGRAMMING

Article Open access 07 September 2020 OPTOGENETIC CONTROL OF THE _LAC_ OPERON FOR BACTERIAL CHEMICAL AND PROTEIN PRODUCTION Article 07 September 2020 REFERENCES * Adler, K., _et al._,

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Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institut für Genetik der Universität zu Köln, 5 Köln 41, Weyertal, 121, West Germany B.

MüLLER-HILL & J. KANIA Authors * B. MüLLER-HILL View author publications You can also search for this author inPubMed Google Scholar * J. KANIA View author publications You can also

search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE MüLLER-HILL, B., KANIA, J. _Lac_ repressor can be fused to

β-galactosidase. _Nature_ 249, 561–563 (1974). https://doi.org/10.1038/249561a0 Download citation * Received: 27 December 1973 * Revised: 19 March 1974 * Issue Date: 07 June 1974 * DOI:

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