ABSTRACT CALCIUM ions are important in almost all membrane fusion systems1–2. Murayama and Okada3 showed that Sendai virus-induced fusion of Ehrlich ascites cells required Ca2+. When Ehrlich

ascites cells were treated with Sendai virus in the presence of EDTA, cells agglutinated and lysed but did not fuse3,4. Similarly, Ca2+ was required for the fusion of erythrocytes

stimulated by chemicals such as lysolecithin5 or glyceryl monooleate6. Ca2+ plus the ionosphere A23187 (ref. 7) or Ca2+ at high _p_H (ref. 8) promoted the fusion of chicken erythrocytes.

Fusion of phosphatidylserine-rich liposomes was absolutely dependent on the presence of Ca2+9. We describe here the fusion of human erythrocyte ghosts promoted by the combined action of Ca2+

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support SIMILAR CONTENT BEING VIEWED BY OTHERS A SUPRAMOLECULAR SYSTEM MIMICKING THE INFECTION PROCESS OF AN ENVELOPED VIRUS THROUGH MEMBRANE FUSION Article Open access 15 November 2023

STRUCTURAL TRANSITION OF GP64 TRIGGERED BY A PH-SENSITIVE MULTI-HISTIDINE SWITCH Article Open access 03 September 2024 ARCHITECTURE OF THE HUMAN ERYTHROCYTE ANKYRIN-1 COMPLEX Article 14 July

2022 REFERENCES * Okada, Y., in _Current Topics in Microbiology and Immunology_, 48 (edit. by Arber, W., and Braun, W.), 102–128 (Springer, Berlin, 1969). Google Scholar  * Poste, G., and

Allison, A. C., _Biochim. biophys. Acta_, 300, 421–465 (1973). Article  CAS  Google Scholar  * Murayama, F., and Okada, Y., _Biken's J._, 8, 103–105 (1965). CAS  Google Scholar  *

Okada, Y., Murayama, F., _Expl Cell Res._, 44, 527–551 (1966). Article  CAS  Google Scholar  * Poole, A. R., Howell, J. I., and Lucy, J. A., _Nature_, 227, 810–813 (1970). Article  CAS  ADS

  Google Scholar  * Ahkong, Q. F., Fisher, D., Tampion, W., and Lucy, J. A., _Biochem. J._, 136, 147–155 (1973). Article  CAS  Google Scholar  * Ahkong, Q. F., Tampion, W., and Lucy, J. A.,

_Nature_, 256, 208–209 (1975). Article  CAS  ADS  Google Scholar  * Toister, Z., and Loyter, A., _Biochim. biophys. Acta_, 241, 719–724 (1971). Article  CAS  Google Scholar  *

Papahadjopoulos, D., Poste, G., Schaeffer, B. E., and Vail, W. J., _Biochim. biophys. Acta_, 352, 10–28 (1974). Article  CAS  Google Scholar  * Seeman, P., _J Cell Biol._, 32, 55–70 (1967).

Article  CAS  Google Scholar  * Loyter, A., Zakai, N., and Kulka, R. G., _J. Cell Biol._, 66, 292–304 (1975). Article  CAS  Google Scholar  * Graham, F. L., and Van der Eb, A. J.,

_Virology_, 52, 456–467 (1973). Article  CAS  Google Scholar  * Romero, P. J., and Whittam, R., _J. Physiol., Lond._, 214, 481–507 (1971). Article  CAS  Google Scholar  * Olson, E. J., and

Cazort, R. J., _J. gen. Physiol._, 53, 311–322 (1969). Article  CAS  Google Scholar  * Peretz, H., Toister, Z., Laster, Y., and Loyter, A., _J. Cell Biol._, 63, 1–11 (1974). Article  CAS 

Google Scholar  * Michaeli, D., Lalazar, A., and Loyter, A., _Israel J. med. Sci._, 11, 1185 (1975). CAS  PubMed  Google Scholar  * Elgsaeter, A., Shotton, D. M., and Branton, D., _Biochim.

biophys. Acta_, 426, 101–122 (1976). Article  CAS  Google Scholar  * Satir, R., in _Symp. Soc. exp. Biol._, 28 (edit. by Sleigh, M. A., and Jennings, D. H.), 399–418 (Cambridge University

Press, London, 1974). Google Scholar  * Lucy, J. A., _Nature_, 227, 814–817 (1970). Article  ADS  Google Scholar  * Gazitt, Y., Loyter, A., Reichler, Y., and Ohad, I., _Biochim. biophys.

Acta_, 419, 479–492 (1976). Article  CAS  Google Scholar  * Gazitt, Y., Ohad, I., and Loyter, A., _Biochim. biophys. Acta_ (in the press). * Rubin, R. P., _Pharmac. Rev._, 22, 389–428

(1970). CAS  Google Scholar  * Allison, A. C., and Davies, P., in _Sym. Soc. exp. Biol._, 28 (edit. by Sleigh, M. A., and Jennings, D. H.), 419–446 (Cambridge University Press, London,

1974). Google Scholar  * Shainberg, A., Yagil, G., and Yaffe, D., _Expl Cell Res._, 58, 163–167 (1969). Article  CAS  Google Scholar  * Gratzl, M., and Dahl, G., _FEBS Lett._, 62, 142–145

(1976). Article  CAS  Google Scholar  * Gazitt, Y., Ohad, I., and Loyter, A., _Biochim. biophys. Acta_, 382, 65–72 (1975). Article  CAS  Google Scholar  * Toister, Z., and Loyter, A., _J.

biol. Chem._, 248, 422–432 (1973). CAS  PubMed  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biological Chemistry, Hebrew University of

Jerusalem, Jerusalem, Israel NEHAMA ZAKAI, RICHARD G. KULKA & ABRAHAM LOYTER Authors * NEHAMA ZAKAI View author publications You can also search for this author inPubMed Google Scholar *

RICHARD G. KULKA View author publications You can also search for this author inPubMed Google Scholar * ABRAHAM LOYTER View author publications You can also search for this author inPubMed 

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combined action of calcium and phosphate ions. _Nature_ 263, 696–699 (1976). https://doi.org/10.1038/263696a0 Download citation * Received: 17 June 1976 * Accepted: 26 July 1976 * Published:

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