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ABSTRACT PMA (phorbol myristate acetate) is the most active of a class of compounds present in croton oil which act as tumour promoters1–3. While PMA possesses little car-cinogenicity in its
own night, its repeated application to mouse skin causes tumour induction in animals treated up to several months previously with a single subthreshold dose of a carcinogen. If the order of
treatments is reversed, that is, if PMA treatment precedes that with the carcinogen, then no tumours are induced. Studies both with mouse skin and with cells in tissue culture indicate
substantial effects of PMA on cell behaviour and biochemistry. Many of these effects such as alteration of phospholipid synthesis4 or stimulation of Na+, K+-ATPase and 5′-nucleotidase5
relate to the cell membrane. We report here that PMA alters the cell surface protein composition of chick embryo fibroblasts, causing a decrease in the major surface protein LETS (molecular
weight 250,000). Previous work by several laboratories has indicated that LETS, which is present on the surface of fibroblasts from a variety of species, either decreases or disappears upon
transformation6–11. Although LETS is highly sensitive to cleavage by proteases such as plasmin12, its mechanism of loss following transformation remains to be clarified. Access through your
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(1976). CAS PubMed Google Scholar Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pharmacology, Harvard Medical School, Boston, Massachusetts, 02115 PETER
M. BLUMBERG, PAUL E. DRIEDGER & PETER W. ROSSOW Authors * PETER M. BLUMBERG View author publications You can also search for this author inPubMed Google Scholar * PAUL E. DRIEDGER View
author publications You can also search for this author inPubMed Google Scholar * PETER W. ROSSOW View author publications You can also search for this author inPubMed Google Scholar RIGHTS
AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE BLUMBERG, P., DRIEDGER, P. & ROSSOW, P. Effect of a phorbol ester on a transformation-sensitive surface
protein of chick fibroblasts. _Nature_ 264, 446–447 (1976). https://doi.org/10.1038/264446a0 Download citation * Received: 12 July 1976 * Accepted: 12 October 1976 * Issue Date: 02 December
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