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ABSTRACT AN important problem pertinent to the mechanism of lymphocyte stimulation with a nonspecific mitogen is how long lymphocytes must be exposed to the mitogen to attain maximal
stimulation. In the case of the stimulation of human and murine lymphocytes by concanavalin A (con A), the cells are irreversibly committed to stimulation if the lectin is (present on the
cell membranes for some 18–20 h after the start of the culture1–3. Here we report studies on the kinetics of the stimulation of mouse splenic lymphocytes by con A. Our data indicate that two
pulses of con A (0–3 h and 15–18 h after the start of the culture) can stimulate the cells to the same degree as the continuous exposure of the cells to con A for 18 h, suggesting that two
signals are necessary for the con A stimulation of lymphocytes. We also show that the first signal is possibly given by the influx of Ca2+. Access through your institution Buy or subscribe
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Scholar Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo,
Bunkyo-ku, Tokyo, 113, Japan SATOSHI TOYOSHIMA, MAKOTO IWATA & TOSHIAKI OSAWA Authors * SATOSHI TOYOSHIMA View author publications You can also search for this author inPubMed Google
Scholar * MAKOTO IWATA View author publications You can also search for this author inPubMed Google Scholar * TOSHIAKI OSAWA View author publications You can also search for this author
inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE TOYOSHIMA, S., IWATA, M. & OSAWA, T. Kinetics of lymphocyte stimulation by
concanavalin A. _Nature_ 264, 447–449 (1976). https://doi.org/10.1038/264447a0 Download citation * Received: 24 May 1976 * Accepted: 04 October 1976 * Issue Date: 02 December 1976 * DOI:
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