Kinetics of lymphocyte stimulation by concanavalin a

Kinetics of lymphocyte stimulation by concanavalin a

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ABSTRACT AN important problem pertinent to the mechanism of lymphocyte stimulation with a nonspecific mitogen is how long lymphocytes must be exposed to the mitogen to attain maximal stimulation. In the case of the stimulation of human and murine lymphocytes by concanavalin A (con A), the cells are irreversibly committed to stimulation if the lectin is (present on the cell membranes for some 18–20 h after the start of the culture1–3. Here we report studies on the kinetics of the stimulation of mouse splenic lymphocytes by con A. Our data indicate that two pulses of con A (0–3 h and 15–18 h after the start of the culture) can stimulate the cells to the same degree as the continuous exposure of the cells to con A for 18 h, suggesting that two signals are necessary for the con A stimulation of lymphocytes. We also show that the first signal is possibly given by the influx of Ca2+. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS STRUCTURE, BIOSYNTHESIS AND REGULATION OF THE T1 ANTIGEN, A PHASE-VARIABLE SURFACE POLYSACCHARIDE CONSERVED IN MANY _SALMONELLA_ SEROVARS Article Open access 02 August 2024 COUNTING GENERATIONS IN BIRTH AND DEATH PROCESSES WITH COMPETING ERLANG AND EXPONENTIAL WAITING TIMES Article Open access 04 July 2022 AN UPDATED VIEW INTO THE CELL CYCLE KINETICS OF HUMAN T LYMPHOCYTES AND THE IMPACT OF IRRADIATION Article Open access 10 May 2022 REFERENCES * Lindahl-Kiessling, K., _Expl Cell Res._, 70, 17–26 (1972). Article  CAS  Google Scholar  * Novogrodsky, A., and Katchalski, E., _Biochim. biophys. Acta_, 228, 579–583 (1971). Article  CAS  Google Scholar  * Gunter, G. R., Wang, J. L., and Edelman, G. M., _J. Cell Biol._, 62, 366–377 (1974). Article  Google Scholar  * Weber, Th. H., Skoog, V. T., Mattsson, A., and Lindahl-Kiessling, K., _Expl Cell Res._, 85, 351–361 (1974). Article  CAS  Google Scholar  * Kawaguchi, T., Matsumoto, I., and Osawa, T., _J. biol Chem._, 249, 2786–2792 (1974). CAS  PubMed  Google Scholar  * Toyoshima, S., Akiyama, Y., Nakano, K., Tonomura, A., and Osawa, T., _Biochemistry_, 10, 4457–4463 (1971). Article  CAS  Google Scholar  * Parker, C. W., _Biochem. biophys. Res. Commun._, 61, 1180–1186 (1974). Article  CAS  Google Scholar  * Freedman, M. H., Raff, M. C., and Gomperts, B., _Nature_, 255, 378–382 (1975). Article  ADS  CAS  Google Scholar  * Maino, V. C., Green, N. M., and Crumpton, M. J., _Nature_, 251, 324–327 (1974). Article  ADS  CAS  Google Scholar  * Luckasen, J. R., White, J. G., and Kersey, J. H., _Proc. natn. Acad. Sci. U.S.A._, 71, 5088–5090 (1974). Article  ADS  CAS  Google Scholar  * Hovi, T., Allison, A. C., and Williams, S. C., _Expl Cell Res._, 96, 92–100 (1976). Article  Google Scholar  * Katagiri, T., Terao, T., and Osawa, T., _J. Biochem. (Tokyo)_, 79, 849–852 (1976). Article  CAS  Google Scholar  * Gillespie, G., _J. Cell Biol._, 50, 544–549 (1971). Article  CAS  Google Scholar  * Gallin, J. I., and Rosenthal, A. S., _J. Cell Biol._, 62, 594–609 (1974). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan SATOSHI TOYOSHIMA, MAKOTO IWATA & TOSHIAKI OSAWA Authors * SATOSHI TOYOSHIMA View author publications You can also search for this author inPubMed Google Scholar * MAKOTO IWATA View author publications You can also search for this author inPubMed Google Scholar * TOSHIAKI OSAWA View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE TOYOSHIMA, S., IWATA, M. & OSAWA, T. Kinetics of lymphocyte stimulation by concanavalin A. _Nature_ 264, 447–449 (1976). https://doi.org/10.1038/264447a0 Download citation * Received: 24 May 1976 * Accepted: 04 October 1976 * Issue Date: 02 December 1976 * DOI: https://doi.org/10.1038/264447a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT AN important problem pertinent to the mechanism of lymphocyte stimulation with a nonspecific mitogen is how long lymphocytes must be exposed to the mitogen to attain maximal


stimulation. In the case of the stimulation of human and murine lymphocytes by concanavalin A (con A), the cells are irreversibly committed to stimulation if the lectin is (present on the


cell membranes for some 18–20 h after the start of the culture1–3. Here we report studies on the kinetics of the stimulation of mouse splenic lymphocytes by con A. Our data indicate that two


pulses of con A (0–3 h and 15–18 h after the start of the culture) can stimulate the cells to the same degree as the continuous exposure of the cells to con A for 18 h, suggesting that two


signals are necessary for the con A stimulation of lymphocytes. We also show that the first signal is possibly given by the influx of Ca2+. Access through your institution Buy or subscribe


This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access


$199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are


calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS


STRUCTURE, BIOSYNTHESIS AND REGULATION OF THE T1 ANTIGEN, A PHASE-VARIABLE SURFACE POLYSACCHARIDE CONSERVED IN MANY _SALMONELLA_ SEROVARS Article Open access 02 August 2024 COUNTING


GENERATIONS IN BIRTH AND DEATH PROCESSES WITH COMPETING ERLANG AND EXPONENTIAL WAITING TIMES Article Open access 04 July 2022 AN UPDATED VIEW INTO THE CELL CYCLE KINETICS OF HUMAN T


LYMPHOCYTES AND THE IMPACT OF IRRADIATION Article Open access 10 May 2022 REFERENCES * Lindahl-Kiessling, K., _Expl Cell Res._, 70, 17–26 (1972). Article  CAS  Google Scholar  * Novogrodsky,


A., and Katchalski, E., _Biochim. biophys. Acta_, 228, 579–583 (1971). Article  CAS  Google Scholar  * Gunter, G. R., Wang, J. L., and Edelman, G. M., _J. Cell Biol._, 62, 366–377 (1974).


Article  Google Scholar  * Weber, Th. H., Skoog, V. T., Mattsson, A., and Lindahl-Kiessling, K., _Expl Cell Res._, 85, 351–361 (1974). Article  CAS  Google Scholar  * Kawaguchi, T.,


Matsumoto, I., and Osawa, T., _J. biol Chem._, 249, 2786–2792 (1974). CAS  PubMed  Google Scholar  * Toyoshima, S., Akiyama, Y., Nakano, K., Tonomura, A., and Osawa, T., _Biochemistry_, 10,


4457–4463 (1971). Article  CAS  Google Scholar  * Parker, C. W., _Biochem. biophys. Res. Commun._, 61, 1180–1186 (1974). Article  CAS  Google Scholar  * Freedman, M. H., Raff, M. C., and


Gomperts, B., _Nature_, 255, 378–382 (1975). Article  ADS  CAS  Google Scholar  * Maino, V. C., Green, N. M., and Crumpton, M. J., _Nature_, 251, 324–327 (1974). Article  ADS  CAS  Google


Scholar  * Luckasen, J. R., White, J. G., and Kersey, J. H., _Proc. natn. Acad. Sci. U.S.A._, 71, 5088–5090 (1974). Article  ADS  CAS  Google Scholar  * Hovi, T., Allison, A. C., and


Williams, S. C., _Expl Cell Res._, 96, 92–100 (1976). Article  Google Scholar  * Katagiri, T., Terao, T., and Osawa, T., _J. Biochem. (Tokyo)_, 79, 849–852 (1976). Article  CAS  Google


Scholar  * Gillespie, G., _J. Cell Biol._, 50, 544–549 (1971). Article  CAS  Google Scholar  * Gallin, J. I., and Rosenthal, A. S., _J. Cell Biol._, 62, 594–609 (1974). Article  CAS  Google


Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo,


Bunkyo-ku, Tokyo, 113, Japan SATOSHI TOYOSHIMA, MAKOTO IWATA & TOSHIAKI OSAWA Authors * SATOSHI TOYOSHIMA View author publications You can also search for this author inPubMed Google


Scholar * MAKOTO IWATA View author publications You can also search for this author inPubMed Google Scholar * TOSHIAKI OSAWA View author publications You can also search for this author


inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE TOYOSHIMA, S., IWATA, M. & OSAWA, T. Kinetics of lymphocyte stimulation by


concanavalin A. _Nature_ 264, 447–449 (1976). https://doi.org/10.1038/264447a0 Download citation * Received: 24 May 1976 * Accepted: 04 October 1976 * Issue Date: 02 December 1976 * DOI:


https://doi.org/10.1038/264447a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently


available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative