ABSTRACT CARBOCYANINE dyes are fluorescent probes of membrane potential in red cells1,2, and their application has been extended to Ehrlich ascites cells3,4 and phytoplankton5. I have used

them to study the effect of potassium concentration gradients on the membrane potential of resealed ghosts containing Ca buffers. Intracellular Ca specifically renders these ghosts highly

permeable to K (ref. 6). During this work, I discovered that 3,3′-dipropyl- and 3,3′-diethyl-thiadicarbocyanine iodide cause a progressive inhibition of K efflux at low external K

concentrations, and that external K protects against the inhibitory effects of the dyes. The results suggest that the carbocyanine dyes are specific inhibitors of the Ca-dependent K

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Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS CYTOPLASMIC ANION/CATION IMBALANCES APPLIED ACROSS THE MEMBRANE CAPACITANCE MAY FORM A SIGNIFICANT COMPONENT OF THE RESTING

MEMBRANE POTENTIAL OF RED BLOOD CELLS Article Open access 02 September 2022 STRUCTURE AND MECHANISM OF THE K+/H+ EXCHANGER KEFC Article Open access 04 June 2024 THE STRUCTURAL BASIS OF THE

PH-HOMEOSTASIS MEDIATED BY THE CL−/HCO3− EXCHANGER, AE2 Article Open access 31 March 2023 REFERENCES * Sims, P. J., Waggoner, A. S., Wang, C.-H., and Hoffman, J. F., _Biochemistry_, 13,

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University of London King's College, Strand, London, WC2R 2LS, UK T. J. B. SIMONS Authors * T. J. B. SIMONS View author publications You can also search for this author inPubMed Google

Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE SIMONS, T. Carbocyanine dyes inhibit Ca-dependent K efflux from human red cell ghosts. _Nature_

264, 467–469 (1976). https://doi.org/10.1038/264467a0 Download citation * Received: 05 August 1976 * Accepted: 04 October 1976 * Issue Date: 02 December 1976 * DOI:

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