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ABSTRACT RNA-CONTAINING type-C viruses with the ability to induce morphological transformation of fibroblasts in cell culture have been isolated from both avian and mammalian species. In
studies on such viruses, clonal isolates of the Rous sarcoma virus have been identified which are competent both for replication and for transformation1. In contrast, in mammalian systems
virtually all strains of sarcoma viruses thus far isolated are defective by themselves for replication2. In order to obtain infectious sarcoma virus, helper type-C viruses must also be
present to provide functions necessary for replication. To analyse the RNA subunits contained in heterologous type-C virus pseudotypes of defective sarcoma viruses, we have studied the RNA
components obtained from a virus complex containing the woolly sarcoma virus and a mouse type-C helper virus. We have observed that the 45 to 70S high molecular weight RNA formed in this
virus complex represents two distinct populations of high molecular weight RNA. One population is composed of subunits of mouse viral RNA, and the other population is composed of subunits of
woolly sarcoma virus RNA. These two sets of apparent dimers3–5 have been readily separated from each other in sucrose gradients, thus providing a rapid, simple approach to obtaining large
quantities of sarcoma virus RNA free of helper virus RNA. Using this approach, the nucleic acid sequences of the woolly sarcoma virus have been analysed. Access through your institution Buy
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OTHERS STRUCTURE OF THE MATURE ROUS SARCOMA VIRUS LATTICE REVEALS A ROLE FOR IP6 IN THE FORMATION OF THE CAPSID HEXAMER Article Open access 28 May 2021 VISUALIZING MOLECULAR INTERACTIONS
THAT DETERMINE ASSEMBLY OF A BULLET-SHAPED VESICULAR STOMATITIS VIRUS PARTICLE Article Open access 15 August 2022 THE STRUCTURE OF A VIRUS-ENCODED NUCLEOSOME Article 29 April 2021 REFERENCES
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Tumor Virus Genetics, National Cancer Institute, Bethesda, Maryland, 20014 EDWARD M. SCOLNICK, DAVID WILLIAMS & WADE P. PARKS Authors * EDWARD M. SCOLNICK View author publications You
can also search for this author inPubMed Google Scholar * DAVID WILLIAMS View author publications You can also search for this author inPubMed Google Scholar * WADE P. PARKS View author
publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE SCOLNICK, E., WILLIAMS, D. &
PARKS, W. Purification and characterization of viral RNA of a sarcoma virus isolated from a woolly monkey. _Nature_ 264, 809–811 (1976). https://doi.org/10.1038/264809a0 Download citation *
Received: 02 September 1976 * Accepted: 26 October 1976 * Issue Date: 23 December 1976 * DOI: https://doi.org/10.1038/264809a0 SHARE THIS ARTICLE Anyone you share the following link with
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