ABSTRACT RNA-CONTAINING type-C viruses with the ability to induce morphological transformation of fibroblasts in cell culture have been isolated from both avian and mammalian species. In

studies on such viruses, clonal isolates of the Rous sarcoma virus have been identified which are competent both for replication and for transformation1. In contrast, in mammalian systems

virtually all strains of sarcoma viruses thus far isolated are defective by themselves for replication2. In order to obtain infectious sarcoma virus, helper type-C viruses must also be

present to provide functions necessary for replication. To analyse the RNA subunits contained in heterologous type-C virus pseudotypes of defective sarcoma viruses, we have studied the RNA

woolly sarcoma virus RNA. These two sets of apparent dimers3–5 have been readily separated from each other in sucrose gradients, thus providing a rapid, simple approach to obtaining large

quantities of sarcoma virus RNA free of helper virus RNA. Using this approach, the nucleic acid sequences of the woolly sarcoma virus have been analysed. Access through your institution Buy

or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and

online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes

which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY

OTHERS STRUCTURE OF THE MATURE ROUS SARCOMA VIRUS LATTICE REVEALS A ROLE FOR IP6 IN THE FORMATION OF THE CAPSID HEXAMER Article Open access 28 May 2021 VISUALIZING MOLECULAR INTERACTIONS

THAT DETERMINE ASSEMBLY OF A BULLET-SHAPED VESICULAR STOMATITIS VIRUS PARTICLE Article Open access 15 August 2022 THE STRUCTURE OF A VIRUS-ENCODED NUCLEOSOME Article 29 April 2021 REFERENCES

* Vogt, R. K., _Virology_, 46, 939–946 (1971). Article  CAS  PubMed  Google Scholar  * Scolnick, E. M., Goldberg, R., and Parks, W., _Cold Spring Harb. Symp. quant. Biol._, 39, 885–895

(1975). Article  Google Scholar  * Kung, H. J., Bailey, J. M., Davidson, N., Nicholson, M. D., and McAllister, R. M., _J. Virol._, 16, 397–411 (1976). Google Scholar  * Bender, W., and

Davidson, N., _Cell._, 7, 595–607 (1976). Article  CAS  PubMed  Google Scholar  * Delius, H., Duesberg, P. H., and Mangel, W. F., _Cold Spring Harb. Symp. quant. Biol._, 39, 835–843 (1974).

Article  Google Scholar  * Theilin, G. H., Gould, D., Fowler, M., and Dungworth, D., _J. natn. Cancer Inst._, 47, 881–889 (1971). Google Scholar  * Wolfe, L. G., Smith, R. V., Deinhardt, F.,

_J. natn. Cancer Inst._, 48, 1905–1908 (1972). CAS  Google Scholar  * Scolnick, E. M., and Parks, W. P., _Int. J. Cancer_, 12, 138–147 (1973). Article  CAS  PubMed  Google Scholar  *

Aaronson, S. A., Stephenson, J. R., Hino, S., and Tronick, S. R., _J. Virol._, 16, 1117–1123 (1975). CAS  PubMed  PubMed Central  Google Scholar  * Hartley, J., and Rowe, W., _J. Virol._,

19, 19–36 (1976). CAS  PubMed  PubMed Central  Google Scholar  * Scolnick, E. M., Parks, W., Kawakami, T., Kohne, D., Okabe, A., Gilden, R., and Hatanaka, M., _J. Virol._, 13, 363–369

(1974). CAS  PubMed  PubMed Central  Google Scholar  * Scolnick, E. M., Howk, R. S., Anisowicz, A., Peebles, P. T., Scher, C. D., and Parks, W. P., _Proc. natn. Acad. Sci., U.S.A._, 72,

4650–4654 (1975). Article  ADS  CAS  Google Scholar  * Stehelin, D., Varmus, H., Bishop, J., and Vogt, P., _Nature_, 260, 170–173 (1976). Article  ADS  CAS  PubMed  Google Scholar  * Parks,

W. P., Howk, R. S., Anisowicz, A., and Scolnick, E. M., _J. Virol._, 18, 491–503 (1976). CAS  PubMed  PubMed Central  Google Scholar  * Maisel, J., Klement, V., Lai, M.M.C., Ostertag, W.,

and Duesberg, P., _Proc. natn. Acad. Sci. U.S.A._, 70, 3536–3540 (1973). Article  ADS  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Laboratory of

Tumor Virus Genetics, National Cancer Institute, Bethesda, Maryland, 20014 EDWARD M. SCOLNICK, DAVID WILLIAMS & WADE P. PARKS Authors * EDWARD M. SCOLNICK View author publications You

can also search for this author inPubMed Google Scholar * DAVID WILLIAMS View author publications You can also search for this author inPubMed Google Scholar * WADE P. PARKS View author

publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE SCOLNICK, E., WILLIAMS, D. &

PARKS, W. Purification and characterization of viral RNA of a sarcoma virus isolated from a woolly monkey. _Nature_ 264, 809–811 (1976). https://doi.org/10.1038/264809a0 Download citation *

Received: 02 September 1976 * Accepted: 26 October 1976 * Issue Date: 23 December 1976 * DOI: https://doi.org/10.1038/264809a0 SHARE THIS ARTICLE Anyone you share the following link with

will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt

content-sharing initiative