Interaction of dna and liposomes as a model for membrane-mediated dna damage

Interaction of dna and liposomes as a model for membrane-mediated dna damage

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ABSTRACT WE have described the antioxidant activity of calf thymus DNA on the oxidation of liposomal suspensions1; we now report the damaging consequences to the biological activity of bacterial transforming DNA in such a system. Because DNA is associated with eukaryotic2 and bacterial cell membranes3, the DNA–membrane interaction described here may be relevant to several pathological processes, including carcinogenesis4–7, ageing8–11 and oxygen-dependent, radiation-induced cell lethality10,11. In the presence of oxygen, the unsaturated fatty chains of membrane phospholipid undergo autoxidation12. These autocatalytic free radical reactions, due to their chain nature, both amplify and propagate an initiating event occurring within the membrane, during which time many highly reactive and potentially damaging chemical species are formed13. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS BREAKING THE DNA BY SOFT X-RAYS IN THE WATER WINDOW REVEALS THE SCAVENGING AND TEMPORAL BEHAVIOUR OF ·OH RADICALS Article Open access 18 November 2024 DIRECTLY IMAGING EMERGENCE OF PHASE SEPARATION IN PEROXIDIZED LIPID MEMBRANES Article Open access 17 January 2023 PHOTOBIOCHEMICAL MECHANISMS OF BIOMOLECULES RELEVANT TO GERMICIDAL ULTRAVIOLET IRRADIATION AT 222 AND 254 NM Article Open access 29 October 2022 REFERENCES * Pietronigro, D. D., Seligman, M. L., Jones, W. B. G. & Demopoulos, H. B. _Lipids_ 11, 808–813 (1976). Article  CAS  PubMed  Google Scholar  * Franke, W. W. & Scheer, U. in _The Cell Nucleus_ (ed. Busch, H.) 1, 219–347 (1974). Book  Google Scholar  * Sueoka, M. & Hammer, J. B. _Proc. natn. Acad. Sci. U.S.A._ 71, 4787–4791 (1974). Article  ADS  CAS  Google Scholar  * Kubinski, H., Strangstalien, M. A., Baird, W. M. & Boutwell, R. K. _Cancer Res._ 33, 3103–3107 (1973). CAS  PubMed  Google Scholar  * Kubinski, H., Anderson, P. R. & Kellicut, L. M. _Chemico-Biol. Interactions_ 5, 279–283 (1972). Article  CAS  Google Scholar  * Kubinski, H. & Kasper, C. B. _Science_ 171, 201–203 (1971). Article  ADS  CAS  PubMed  Google Scholar  * Tafefel'shtein, E. Ye., Utkin, O. B. & Vladimirov, Yu. A. _Biofizika_ 16, 949–951 (1971). Google Scholar  * Munkres, K. D. _Mech. Aging Devel._ 5, 171–191 (1976). Article  CAS  Google Scholar  * Cutler, R. G. in _Aging, Carcinogenesis and Radiation Biology_ (ed. Smith, K. C.) 443–492 (1976). Book  Google Scholar  * Alper, T. _Proc. second Symp. Microdosimetry_, Stresa (ed. Ebert, H. G.) Eur 4452-d-f-c, 5 (Commission of European Communities, Brussels, 1970). Google Scholar  * Alper, T. _Nature_ 217, 862–863 (1968) Article  ADS  CAS  PubMed  Google Scholar  * Demopoulos, H. B. _Fedn Proc._ 32, 1903–1908 (1973); _Ibid._ 1859–1861 (1973). CAS  Google Scholar  * Wolman, M. _Irs. J. Med. Sci._ 11, Suppl. (1975). * Schaich, K. M. & Karel, M. _Lipids_ 11, 392–400 (1976). Article  CAS  PubMed  Google Scholar  * Okada, S. _Radiation Biochemistry_ 1, 23–26, 51 (Academic, New York, 1970). Google Scholar  * Reiss, U., Tappel, A. L. & Chio, K. S. _Biochem. Biophys. Res. Commun._ 48, 921–926 (1972). Article  CAS  PubMed  Google Scholar  * Reiss, U. & Tappel, A. L. _Lipids_ 8, 199–202 (1973). Article  CAS  PubMed  Google Scholar  * Ebert, M. & Hornsey, S. _Nature_ 181, 613–616 (1958). Article  ADS  CAS  PubMed  Google Scholar  * Shenoy, M. A., Asquith, J. C., Adams, G. E., Micheal, B. D. & Watts, M. E. _Radiat. Res._ 62, 498–512 (1975). Article  ADS  CAS  PubMed  Google Scholar  * Cramp, W. A., Watkins, D. K. & Collins, J. _Nature new Biol._ 235, 76–77 (1972). Article  CAS  PubMed  Google Scholar  * Gebicki, J. M. & Allen, A. O. _J. phys. Chem._ 73, 2443–2445 (1969). Article  CAS  Google Scholar  * Lawrence, J. H., Loomis, W. F., Tobias, C. A. & Turpin, F. H. _J. Physiol._ 105, 197–204 (1946). Article  CAS  PubMed  PubMed Central  Google Scholar  * Kotin, P. & Falk, H. L. _Radiat. Res._ Suppl. 3, 193–211 (1963). * Mukai, F. H. & Goldstein, B. D. _Science_ 191, 868–869 (1976). Article  ADS  CAS  PubMed  Google Scholar  * Schamberger, R. J., Andreone, T. L. & Willis, C. E. _J. natn. Ca. Inst._ 53, 1771–1773 (1974). Google Scholar  * Tappel, A. L. in _Pathobiology of Cell Membranes_ (eds Trump, B. F. & Arstila, A. V.) 1, 145–172 (1975). Book  Google Scholar  * Mavis, R. D. & Vagelos, P. R. _J. biol. Chem._ 247, 652–659 (1972). CAS  PubMed  Google Scholar  * Chang, T. & Vagelos, P. R. _Proc. natn. Acad. Sci._ 73, 24–28 (1976). Article  ADS  CAS  Google Scholar  * Dubnau, D. & Davidoff-Abelson, R. _J. molec. Biol._ 56, 209–221 (1971). Article  CAS  PubMed  Google Scholar  * Satava, J., Zadrazil, S. & Sormova, Z. _Collection Czechoslov. chem. Commun._ 38, 2167–2173 (1973). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * DENNIS D. PIETRONIGRO Present address: Department of Pharmacology, Yale University Medical School, New Haven, Ct, 06510 AUTHORS AND AFFILIATIONS * Department of Pathology, New York University Medical Center, New York, New York, 10016 DENNIS D. PIETRONIGRO, W. BARRIE G. JONES, KATHY KALTY & HARRY B. DEMOPOULOS Authors * DENNIS D. PIETRONIGRO View author publications You can also search for this author inPubMed Google Scholar * W. BARRIE G. JONES View author publications You can also search for this author inPubMed Google Scholar * KATHY KALTY View author publications You can also search for this author inPubMed Google Scholar * HARRY B. DEMOPOULOS View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE PIETRONIGRO, D., JONES, W., KALTY, K. _et al._ Interaction of DNA and liposomes as a model for membrane-mediated DNA damage. _Nature_ 267, 78–79 (1977). https://doi.org/10.1038/267078a0 Download citation * Received: 13 September 1976 * Accepted: 01 March 1977 * Issue Date: 05 May 1977 * DOI: https://doi.org/10.1038/267078a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT WE have described the antioxidant activity of calf thymus DNA on the oxidation of liposomal suspensions1; we now report the damaging consequences to the biological activity of


bacterial transforming DNA in such a system. Because DNA is associated with eukaryotic2 and bacterial cell membranes3, the DNA–membrane interaction described here may be relevant to several


pathological processes, including carcinogenesis4–7, ageing8–11 and oxygen-dependent, radiation-induced cell lethality10,11. In the presence of oxygen, the unsaturated fatty chains of


membrane phospholipid undergo autoxidation12. These autocatalytic free radical reactions, due to their chain nature, both amplify and propagate an initiating event occurring within the


membrane, during which time many highly reactive and potentially damaging chemical species are formed13. Access through your institution Buy or subscribe This is a preview of subscription


content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue


Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL


ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS BREAKING THE DNA BY SOFT X-RAYS IN THE


WATER WINDOW REVEALS THE SCAVENGING AND TEMPORAL BEHAVIOUR OF ·OH RADICALS Article Open access 18 November 2024 DIRECTLY IMAGING EMERGENCE OF PHASE SEPARATION IN PEROXIDIZED LIPID MEMBRANES


Article Open access 17 January 2023 PHOTOBIOCHEMICAL MECHANISMS OF BIOMOLECULES RELEVANT TO GERMICIDAL ULTRAVIOLET IRRADIATION AT 222 AND 254 NM Article Open access 29 October 2022


REFERENCES * Pietronigro, D. D., Seligman, M. L., Jones, W. B. G. & Demopoulos, H. B. _Lipids_ 11, 808–813 (1976). Article  CAS  PubMed  Google Scholar  * Franke, W. W. & Scheer, U.


in _The Cell Nucleus_ (ed. Busch, H.) 1, 219–347 (1974). Book  Google Scholar  * Sueoka, M. & Hammer, J. B. _Proc. natn. Acad. Sci. U.S.A._ 71, 4787–4791 (1974). Article  ADS  CAS 


Google Scholar  * Kubinski, H., Strangstalien, M. A., Baird, W. M. & Boutwell, R. K. _Cancer Res._ 33, 3103–3107 (1973). CAS  PubMed  Google Scholar  * Kubinski, H., Anderson, P. R.


& Kellicut, L. M. _Chemico-Biol. Interactions_ 5, 279–283 (1972). Article  CAS  Google Scholar  * Kubinski, H. & Kasper, C. B. _Science_ 171, 201–203 (1971). Article  ADS  CAS 


PubMed  Google Scholar  * Tafefel'shtein, E. Ye., Utkin, O. B. & Vladimirov, Yu. A. _Biofizika_ 16, 949–951 (1971). Google Scholar  * Munkres, K. D. _Mech. Aging Devel._ 5, 171–191


(1976). Article  CAS  Google Scholar  * Cutler, R. G. in _Aging, Carcinogenesis and Radiation Biology_ (ed. Smith, K. C.) 443–492 (1976). Book  Google Scholar  * Alper, T. _Proc. second


Symp. Microdosimetry_, Stresa (ed. Ebert, H. G.) Eur 4452-d-f-c, 5 (Commission of European Communities, Brussels, 1970). Google Scholar  * Alper, T. _Nature_ 217, 862–863 (1968) Article  ADS


  CAS  PubMed  Google Scholar  * Demopoulos, H. B. _Fedn Proc._ 32, 1903–1908 (1973); _Ibid._ 1859–1861 (1973). CAS  Google Scholar  * Wolman, M. _Irs. J. Med. Sci._ 11, Suppl. (1975). *


Schaich, K. M. & Karel, M. _Lipids_ 11, 392–400 (1976). Article  CAS  PubMed  Google Scholar  * Okada, S. _Radiation Biochemistry_ 1, 23–26, 51 (Academic, New York, 1970). Google Scholar


  * Reiss, U., Tappel, A. L. & Chio, K. S. _Biochem. Biophys. Res. Commun._ 48, 921–926 (1972). Article  CAS  PubMed  Google Scholar  * Reiss, U. & Tappel, A. L. _Lipids_ 8, 199–202


(1973). Article  CAS  PubMed  Google Scholar  * Ebert, M. & Hornsey, S. _Nature_ 181, 613–616 (1958). Article  ADS  CAS  PubMed  Google Scholar  * Shenoy, M. A., Asquith, J. C., Adams,


G. E., Micheal, B. D. & Watts, M. E. _Radiat. Res._ 62, 498–512 (1975). Article  ADS  CAS  PubMed  Google Scholar  * Cramp, W. A., Watkins, D. K. & Collins, J. _Nature new Biol._


235, 76–77 (1972). Article  CAS  PubMed  Google Scholar  * Gebicki, J. M. & Allen, A. O. _J. phys. Chem._ 73, 2443–2445 (1969). Article  CAS  Google Scholar  * Lawrence, J. H., Loomis,


W. F., Tobias, C. A. & Turpin, F. H. _J. Physiol._ 105, 197–204 (1946). Article  CAS  PubMed  PubMed Central  Google Scholar  * Kotin, P. & Falk, H. L. _Radiat. Res._ Suppl. 3,


193–211 (1963). * Mukai, F. H. & Goldstein, B. D. _Science_ 191, 868–869 (1976). Article  ADS  CAS  PubMed  Google Scholar  * Schamberger, R. J., Andreone, T. L. & Willis, C. E. _J.


natn. Ca. Inst._ 53, 1771–1773 (1974). Google Scholar  * Tappel, A. L. in _Pathobiology of Cell Membranes_ (eds Trump, B. F. & Arstila, A. V.) 1, 145–172 (1975). Book  Google Scholar  *


Mavis, R. D. & Vagelos, P. R. _J. biol. Chem._ 247, 652–659 (1972). CAS  PubMed  Google Scholar  * Chang, T. & Vagelos, P. R. _Proc. natn. Acad. Sci._ 73, 24–28 (1976). Article  ADS


  CAS  Google Scholar  * Dubnau, D. & Davidoff-Abelson, R. _J. molec. Biol._ 56, 209–221 (1971). Article  CAS  PubMed  Google Scholar  * Satava, J., Zadrazil, S. & Sormova, Z.


_Collection Czechoslov. chem. Commun._ 38, 2167–2173 (1973). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * DENNIS D. PIETRONIGRO Present address:


Department of Pharmacology, Yale University Medical School, New Haven, Ct, 06510 AUTHORS AND AFFILIATIONS * Department of Pathology, New York University Medical Center, New York, New York,


10016 DENNIS D. PIETRONIGRO, W. BARRIE G. JONES, KATHY KALTY & HARRY B. DEMOPOULOS Authors * DENNIS D. PIETRONIGRO View author publications You can also search for this author inPubMed 


Google Scholar * W. BARRIE G. JONES View author publications You can also search for this author inPubMed Google Scholar * KATHY KALTY View author publications You can also search for this


author inPubMed Google Scholar * HARRY B. DEMOPOULOS View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions


ABOUT THIS ARTICLE CITE THIS ARTICLE PIETRONIGRO, D., JONES, W., KALTY, K. _et al._ Interaction of DNA and liposomes as a model for membrane-mediated DNA damage. _Nature_ 267, 78–79 (1977).


https://doi.org/10.1038/267078a0 Download citation * Received: 13 September 1976 * Accepted: 01 March 1977 * Issue Date: 05 May 1977 * DOI: https://doi.org/10.1038/267078a0 SHARE THIS


ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard


Provided by the Springer Nature SharedIt content-sharing initiative