Rearrangements of t-cell receptor gene yt35 in human dna from thymic leukaemia t-cell lines and functional t-cell clones

Rearrangements of t-cell receptor gene yt35 in human dna from thymic leukaemia t-cell lines and functional t-cell clones

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ABSTRACT An essential property of the immune response is its ability to distinguish between self and non-self and to generate enormous diversity in antibody and T-cell immune responses. Although the genetic and molecular mechanisms responsible for antibody diversity have now largely been elucidated, the structure of the T-cell receptor and the diversification of the receptor repertoire have only recently become amenable to study. One approach has involved immunochemical studies of the protein precipitated by monoclonal antibodies which react specifically with the immunizing T-cell clones1–3. Another approach has been to clone and sequence a human4 or a murine5 T-cell specific message that may specify part of the T-cell receptor. We present here results of Southern blot analysis of non-T, immature T, and mature T-cell genomic DNA, and provide evidence that rearrangements of the _YT35_ sequences do occur in the DNA of thymic leukaemia T cells. This suggests that _YT35_ codes for at least part of the T-cell receptor and that rearrangements occur at this early stage of thymic ontogeny. Furthermore, DNA rearrangements are present in lymphocytes with phenotypic and functional characteristics of helper, killer, or suppressor T cells. We conclude that the three subpopulations of T cells operate via receptor molecules encoded by the same gene family. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS FEATURES OF REPERTOIRE DIVERSITY AND GENE EXPRESSION IN HUMAN CYTOTOXIC T CELLS FOLLOWING ALLOGENEIC HEMATOPOIETIC CELL TRANSPLANTATION Article Open access 11 October 2021 INTEGRATING T CELL RECEPTOR SEQUENCES AND TRANSCRIPTIONAL PROFILES BY CLONOTYPE NEIGHBOR GRAPH ANALYSIS (CONGA) Article 23 August 2021 DUAL T-CELL CONSTANT Β CHAIN (TRBC)1 AND TRBC2 STAINING FOR THE IDENTIFICATION OF T-CELL NEOPLASMS BY FLOW CYTOMETRY Article Open access 29 February 2024 REFERENCES * Acuto, O. _et al._ _Cell_ 34, 717–726 (1983). Article  CAS  Google Scholar  * Kappler, J. _et al._ _Cell_ 34, 727–737 (1983). Article  CAS  Google Scholar  * McIntyre, B. W. & Allison, J. P. _Cell_ 34, 739–746 (1983). Article  CAS  Google Scholar  * Yanagi, Y. _et al._ _Nature_ 308, 145–149 (1984). Article  ADS  CAS  Google Scholar  * Hedricks, S. M., Nielsen, S. M., Kavaler, J., Cohen, D. T. & Davis, M. _Nature_ 308, 153–158 (1984). Article  ADS  Google Scholar  * Southern, E. M. _J. molec. Biol._ 38, 503–517 (1975). Article  Google Scholar  * Suciu-Foca, N., Rohowsky, C., Kung, P. & King, D. W. _J. exp. Med._ 156, 283–288 (1982). Article  CAS  Google Scholar  * Suciu-Foca, N., Rohowsky, C., Kung, P. & King, D. W. _Hum. Immun._ 9, 37–47 (1984). Article  CAS  Google Scholar  * Korsmeyer, S. J. _et al._ _J. clin. Invest._ 71, 301–313 (1983). Article  CAS  Google Scholar  * Honjo, T. A. _Rev. Immun._, 1, 499–528 (1983). Article  CAS  Google Scholar  * Tonegawa, S. _Nature_ 302, 575–581 (1983). Article  ADS  CAS  Google Scholar  * Owen, F. L. _Immunology Today_ 3, 95–96 (1982). Article  CAS  Google Scholar  * Sredni, B. & Schwartz, R. H. _Nature_ 287, 855–857 (1980). Article  ADS  CAS  Google Scholar  * Abromson-Leeman, S. R. & Cantor, M. _J. exp. Med._ 158, 428–437 (1983). Article  CAS  Google Scholar  * Yoshikai, Y. _et al._ _Nature_ 310, 506–508 (1984). Article  ADS  CAS  Google Scholar  * Suciu-Foca, N., Rohowsky, C., Kung, P. & King, D. W. _J. exp. Med._ 156, 283–288 (1982). Article  CAS  Google Scholar  * Krensky, A. M., Reiss, C. S., Mier, J. W., Strominger, J. L. & Burakoff, S. J. _Proc. natn. Acad. Sci. U.S.A._ 79, 2365–2369 (1982). Article  ADS  CAS  Google Scholar  * Fauci, A. S. & Pratt, K. R. _Proc. natn. Acad. Sci. U.S.A._ 73, 3676–3679 (1976). Article  ADS  CAS  Google Scholar  * Yamamoto, Y. _et al._ _Proc. natn. Acad. Sci. U.S.A._ 78, 6893–6897 (1981). Article  ADS  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * Nicole Suciu-Foca: Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10031, USA AUTHORS AND AFFILIATIONS * The Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 500 Sherbourne Street, Toronto, Ontario, Canada, M4X 1K9 Barry Toyonaga, Yusuke Yanagi, Nicole Suciu-Foca, Mark Minden & Tak W. Mak Authors * Barry Toyonaga View author publications You can also search for this author inPubMed Google Scholar * Yusuke Yanagi View author publications You can also search for this author inPubMed Google Scholar * Nicole Suciu-Foca View author publications You can also search for this author inPubMed Google Scholar * Mark Minden View author publications You can also search for this author inPubMed Google Scholar * Tak W. Mak View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Toyonaga, B., Yanagi, Y., Suciu-Foca, N. _et al._ Rearrangements of T-cell receptor gene _YT35_ in human DNA from thymic leukaemia T-cell lines and functional T-cell clones. _Nature_ 311, 385–387 (1984). https://doi.org/10.1038/311385a0 Download citation * Received: 25 April 1984 * Accepted: 23 July 1984 * Issue Date: 27 September 1984 * DOI: https://doi.org/10.1038/311385a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT An essential property of the immune response is its ability to distinguish between self and non-self and to generate enormous diversity in antibody and T-cell immune responses.


Although the genetic and molecular mechanisms responsible for antibody diversity have now largely been elucidated, the structure of the T-cell receptor and the diversification of the


receptor repertoire have only recently become amenable to study. One approach has involved immunochemical studies of the protein precipitated by monoclonal antibodies which react


specifically with the immunizing T-cell clones1–3. Another approach has been to clone and sequence a human4 or a murine5 T-cell specific message that may specify part of the T-cell receptor.


We present here results of Southern blot analysis of non-T, immature T, and mature T-cell genomic DNA, and provide evidence that rearrangements of the _YT35_ sequences do occur in the DNA


of thymic leukaemia T cells. This suggests that _YT35_ codes for at least part of the T-cell receptor and that rearrangements occur at this early stage of thymic ontogeny. Furthermore, DNA


rearrangements are present in lymphocytes with phenotypic and functional characteristics of helper, killer, or suppressor T cells. We conclude that the three subpopulations of T cells


operate via receptor molecules encoded by the same gene family. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS


OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on


SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about


institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS FEATURES OF REPERTOIRE DIVERSITY AND GENE EXPRESSION IN HUMAN CYTOTOXIC T CELLS


FOLLOWING ALLOGENEIC HEMATOPOIETIC CELL TRANSPLANTATION Article Open access 11 October 2021 INTEGRATING T CELL RECEPTOR SEQUENCES AND TRANSCRIPTIONAL PROFILES BY CLONOTYPE NEIGHBOR GRAPH


ANALYSIS (CONGA) Article 23 August 2021 DUAL T-CELL CONSTANT Β CHAIN (TRBC)1 AND TRBC2 STAINING FOR THE IDENTIFICATION OF T-CELL NEOPLASMS BY FLOW CYTOMETRY Article Open access 29 February


2024 REFERENCES * Acuto, O. _et al._ _Cell_ 34, 717–726 (1983). Article  CAS  Google Scholar  * Kappler, J. _et al._ _Cell_ 34, 727–737 (1983). Article  CAS  Google Scholar  * McIntyre, B.


W. & Allison, J. P. _Cell_ 34, 739–746 (1983). Article  CAS  Google Scholar  * Yanagi, Y. _et al._ _Nature_ 308, 145–149 (1984). Article  ADS  CAS  Google Scholar  * Hedricks, S. M.,


Nielsen, S. M., Kavaler, J., Cohen, D. T. & Davis, M. _Nature_ 308, 153–158 (1984). Article  ADS  Google Scholar  * Southern, E. M. _J. molec. Biol._ 38, 503–517 (1975). Article  Google


Scholar  * Suciu-Foca, N., Rohowsky, C., Kung, P. & King, D. W. _J. exp. Med._ 156, 283–288 (1982). Article  CAS  Google Scholar  * Suciu-Foca, N., Rohowsky, C., Kung, P. & King, D.


W. _Hum. Immun._ 9, 37–47 (1984). Article  CAS  Google Scholar  * Korsmeyer, S. J. _et al._ _J. clin. Invest._ 71, 301–313 (1983). Article  CAS  Google Scholar  * Honjo, T. A. _Rev. Immun._,


1, 499–528 (1983). Article  CAS  Google Scholar  * Tonegawa, S. _Nature_ 302, 575–581 (1983). Article  ADS  CAS  Google Scholar  * Owen, F. L. _Immunology Today_ 3, 95–96 (1982). Article 


CAS  Google Scholar  * Sredni, B. & Schwartz, R. H. _Nature_ 287, 855–857 (1980). Article  ADS  CAS  Google Scholar  * Abromson-Leeman, S. R. & Cantor, M. _J. exp. Med._ 158, 428–437


(1983). Article  CAS  Google Scholar  * Yoshikai, Y. _et al._ _Nature_ 310, 506–508 (1984). Article  ADS  CAS  Google Scholar  * Suciu-Foca, N., Rohowsky, C., Kung, P. & King, D. W. _J.


exp. Med._ 156, 283–288 (1982). Article  CAS  Google Scholar  * Krensky, A. M., Reiss, C. S., Mier, J. W., Strominger, J. L. & Burakoff, S. J. _Proc. natn. Acad. Sci. U.S.A._ 79,


2365–2369 (1982). Article  ADS  CAS  Google Scholar  * Fauci, A. S. & Pratt, K. R. _Proc. natn. Acad. Sci. U.S.A._ 73, 3676–3679 (1976). Article  ADS  CAS  Google Scholar  * Yamamoto, Y.


_et al._ _Proc. natn. Acad. Sci. U.S.A._ 78, 6893–6897 (1981). Article  ADS  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * Nicole Suciu-Foca: Department of


Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10031, USA AUTHORS AND AFFILIATIONS * The Ontario Cancer Institute and Department of Medical


Biophysics, University of Toronto, 500 Sherbourne Street, Toronto, Ontario, Canada, M4X 1K9 Barry Toyonaga, Yusuke Yanagi, Nicole Suciu-Foca, Mark Minden & Tak W. Mak Authors * Barry


Toyonaga View author publications You can also search for this author inPubMed Google Scholar * Yusuke Yanagi View author publications You can also search for this author inPubMed Google


Scholar * Nicole Suciu-Foca View author publications You can also search for this author inPubMed Google Scholar * Mark Minden View author publications You can also search for this author


inPubMed Google Scholar * Tak W. Mak View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE


CITE THIS ARTICLE Toyonaga, B., Yanagi, Y., Suciu-Foca, N. _et al._ Rearrangements of T-cell receptor gene _YT35_ in human DNA from thymic leukaemia T-cell lines and functional T-cell


clones. _Nature_ 311, 385–387 (1984). https://doi.org/10.1038/311385a0 Download citation * Received: 25 April 1984 * Accepted: 23 July 1984 * Issue Date: 27 September 1984 * DOI:


https://doi.org/10.1038/311385a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently


available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative