ABSTRACT The effect of complement on transgene expression was evaluated _in vivo_ and _in vitro_ using mice lacking complement components. Complement component 3 (C3) deficient mice (C3−/−)

and appropriate wild-type controls were intravenously injected with a replication incompetent, luciferase-expressing normal Ad5 (Ad5Luc1), or fibritin-fiber Ad5 (Ad5FFLuc1). Repeated,

noninvasive bioluminescence imaging was conducted over 35 days. Our data show for the first time that C3 facilitates both short- and long-term hepatic expression of luciferase following

systemic delivery. C3−/− mice showed significantly less (P<0.05) luciferase expression in their liver than treatment-matched wild-type mice when 2.3 × 109 (Ad5Luc1) and 4.0 × 109 (Ad5Luc1

factor B (FB) deficient mouse sera for 5 min significantly (P<0.05) increased transduction of mouse liver cells, as compared to preincubation with C3−/− sera or PBS. These results suggest

the classical or alternate complement pathway enhances Ad5-mediated liver transduction. Access through your institution Buy or subscribe This is a preview of subscription content, access

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Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS IMPACT OF LIVER FIBROSIS ON AAV-MEDIATED GENE TRANSFER TO

MOUSE HEPATOCYTES Article Open access 10 March 2025 HARNESSING WHOLE HUMAN LIVER EX SITU NORMOTHERMIC PERFUSION FOR PRECLINICAL AAV VECTOR EVALUATION Article Open access 14 March 2024 A

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ACKNOWLEDGEMENTS Research support was derived from NIH Grant Numbers CA80104 and 5P50CA089019, as well as DAMD17-02-01-0266. We thank Glorisa Reason for assistance with liver assays. AUTHOR

INFORMATION AUTHORS AND AFFILIATIONS * Department of Medicine, University of Alabama at Birmingham, Birmingham, 35294, AL, USA KR Zinn & AJ Szalai * Department of Radiology, University

of Alabama at Birmingham, Birmingham, 35294, AL, USA KR Zinn & TR Chaudhuri * The Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, 35294, AL, USA KR Zinn, A

Stargel & TR Chaudhuri * Department of Experimental Diagnostic Imaging, MD Anderson Cancer Center, University of Texas, Houston, 77030, TX, USA V Krasnykh Authors * KR Zinn View author

publications You can also search for this author inPubMed Google Scholar * AJ Szalai View author publications You can also search for this author inPubMed Google Scholar * A Stargel View

author publications You can also search for this author inPubMed Google Scholar * V Krasnykh View author publications You can also search for this author inPubMed Google Scholar * TR

Chaudhuri View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Zinn, K.,

Szalai, A., Stargel, A. _et al._ Bioluminescence imaging reveals a significant role for complement in liver transduction following intravenous delivery of adenovirus. _Gene Ther_ 11,

1482–1486 (2004). https://doi.org/10.1038/sj.gt.3302331 Download citation * Received: 10 July 2003 * Accepted: 18 May 2004 * Published: 05 August 2004 * Issue Date: 01 October 2004 * DOI:

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currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * luciferase * adenovirus * innate immunity * gene

therapy * inflammation * complement