ABSTRACT Recombinant adenoviral vectors (AdV) have been used experimentally as vaccines to present antigenic transgenes _in vivo_. However, administration of first-generation vectors

(FG-AdV) is often limited by their induction of antiviral immunity. To address this limitation, helper-dependent vectors (HD-AdV) were developed that lack viral coding regions. While the

administration of HD-AdV results in long-term gene expression _in vivo_, their utility as immunogens has never been examined. Direct vaccination with 108 blue-forming units (BFU) of HD-AdV

injected into C57BL/6 mice lead to superior transgene-specific CTL and antibody responses when compared to the same amount of a FG-AdV. The antibody responses to viral antigens were high in

stimulated superior transgene-specific CTL responses when administered _in vivo_, an effect that was further enhanced by maturing the DC with LPS prior to administration. In contrast to

direct immunization with HD-AdV, vaccination with HD-AdV-transduced DC was associated with limited antibody responses against the AdV. We conclude that HD-AdV stimulates superior

transgene-specific immune responses when compared to a FG-AdV, and that immunization with a DC-based vaccine maintains this efficacy while limiting antiviral reactivity. Access through your

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BEING VIEWED BY OTHERS OPTIMISING T CELL (RE)BOOSTING STRATEGIES FOR ADENOVIRAL AND MODIFIED VACCINIA ANKARA VACCINE REGIMENS IN HUMANS Article Open access 12 October 2020 LENTIVIRAL VECTOR

INDUCES HIGH-QUALITY MEMORY T CELLS VIA DENDRITIC CELLS TRANSDUCTION Article Open access 10 June 2021 RECOMBINANT MVA-PRIME ELICITS NEUTRALIZING ANTIBODY RESPONSES BY INDUCING

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ACKNOWLEDGEMENTS Supported by grants from the Tobacco-Related Disease Research Program of California (SKB, 10KT-0086; MDR, #7RT-0040); the American Lung Association of California (SKB); the

UCLA Gene Therapy Program (SMK); and the UCLA Lung Cancer SPORE (NIH/NCI 1 P50 CA090388). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Pulmonary & Critical Care Medicine, UCLA School of

Medicine, Los Angeles, CA, USA A Harui, M D Roth, S M Kiertscher & S K Basak * Microbiology, Immunology and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA, USA K Mitani *

Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, CA, USA M D Roth, S M Kiertscher & K Mitani Authors * A Harui View author publications You can also search for

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Vaccination with helper-dependent adenovirus enhances the generation of transgene-specific CTL. _Gene Ther_ 11, 1617–1626 (2004). https://doi.org/10.1038/sj.gt.3302332 Download citation *

Received: 04 September 2003 * Accepted: 03 June 2004 * Published: 05 August 2004 * Issue Date: 01 November 2004 * DOI: https://doi.org/10.1038/sj.gt.3302332 SHARE THIS ARTICLE Anyone you

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Springer Nature SharedIt content-sharing initiative KEYWORDS * dendritic cells * vaccination * rodent * CTL