ABSTRACT Using a liposomal transfection with purified bovine leukemia virus (BLV) integrase, we observed an efficient DNA rearrangement of a chromosomal repeat sequence and targeted

integration of a part of the transfected plasmid. The BLV integrase recognition sequence (IRS) including the 3′ end of the BLV LTR U5, one of the sites cleaved by the integrase, was

essential for the DNA rearrangement, and a sequence homologous to the chromosomal DNA neighboring the repeat target site had to be placed downstream of the IRS on the transfected plasmid.

The pSV2neo DNA, including the pBR322 sequence preintegrated into L929 cells (primary transfectants), was rearranged by a secondary transfection of a pBR322-based hygromycin-resistance

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We thank Professor H Koyama of the Kihara Institute for Biological Research at Yokohama City University for critical reading and helpful discussions. We also thank Mr C Moreby for

preparation of the manuscript. This work was supported in part by a grant from the Foundation of Human Health Sciences. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Safety

Research on Biologics and Blood Products, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan A S Tanaka & K Komuro Authors * A S Tanaka View author publications You can

also search for this author inPubMed Google Scholar * K Komuro View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Tanaka, A., Komuro, K. Targeted rearrangement of a chromosomal repeat sequence by transfection of a homologous DNA sequence using purified

integrase. _Gene Ther_ 12, 783–794 (2005). https://doi.org/10.1038/sj.gt.3302458 Download citation * Received: 22 July 2004 * Accepted: 24 November 2004 * Published: 17 February 2005 * Issue

Date: 01 May 2005 * DOI: https://doi.org/10.1038/sj.gt.3302458 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a

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recognition sequence * repeat sequence * DNA rearrangement * site-specific integration