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Access through your institution Buy or subscribe The new reports show that actin-based motility only occurs close to the cell periphery, and that the formation of actin tails seems to take
place only during or after fusion of viral particles with the plasma membrane. To reach the cell periphery, however, vaccinia particles travel along microtubules in a saltatory fashion. This
is the first example of a virus using both the microtubule and actin cytoskeleton to spread from cell to cell. Rietdorf and colleagues have identified conventional kinesin as the motor that
is responsible for microtubule-based transport, and show that this motor is recruited to viral particles through the tetratricopeptide (TPR) repeats in the kinesin light chain. The A36R
protein, which is encoded by vaccinia and is essential for vaccinia-induced actin polymerization, is also required to recruit kinesin to virus particles and for their movement to the cell
periphery. Interestingly, Rietdorf _et al_. found that virus particles are never associated with both kinesin and actin tails, indicating that the transition from movement along microtubules
to fusion with the plasma membrane and actin-tail formation is tightly regulated. Whether A36R has any role in this, and the nature of the exact molecular link between A36R and
kinesin-dependent movement, will be the subject of future studies. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution
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Contact customer support ORIGINAL RESEARCH PAPER * Ward, B. M. & Moss, B. Visualization of intracellular movement of vaccinia virus virions containing a green fluorescent protein–B5R
membrane protein chimera. _J. Virol._ 75, 4802–4813 (2001) Article CAS Google Scholar * Hollinshead, M. et al. Vaccinia virus ulitilizes microtubules for movement to the cell surface. _J.
Cell Biol._ 154, 389–402 (2001) Article CAS Google Scholar * Rietdorf, J. et al. Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus. _Nature Cell
Biol._ 3, 992–1001 (2001) Article CAS Google Scholar Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Editor, Nature Cell Biology, Barbara Marte Authors * Barbara Marte
View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Marte, B. Vaccinia
virus on the move. _Nat Rev Mol Cell Biol_ 2, 792 (2001). https://doi.org/10.1038/35099061 Download citation * Issue Date: 01 November 2001 * DOI: https://doi.org/10.1038/35099061 SHARE THIS
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