ABSTRACT THE _Notch_ locus of _Drosophila melanogaster_ encodes a 2,703-amino-acid transmembrane protein required for a variety of developmental processes, including neurogenesis, oogenesis

and ommatidial assembly1,2. The Notch protein contains a large extracellular domain of 36 epidermal growth factor-like repeats as well as three Notch/Lin-12 repeats and an intracellular

domain with 6 CdclO/ ankyrin repeats, motifs that are highly conserved in several vertebrate Notch homologues3–9. Truncation of the extracellular domain of the _Drosophila_ Notch protein

produces an activated receptor, as judged by its ability to cause phenotypes similar to gain-of-function alleles or duplications of the _Notch_ locus10. Equivalent truncations of vertebrate

same cells transiently blocks their proper cell-fate commitment, causing them either to adopt incorrect cell fates or to differentiate incompletely. Moreover, an activated Notch protein

lacking the transmembrane domain is translocated to the nucleus, raising the possibility that Notch may participate directly in nuclear events. Access through your institution Buy or

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August 2022 REFERENCES * Artavanis-Tsakonas, S. & Simpson, P. _Trends Genet._ 7, 403–408 (1991). Article  CAS  PubMed  Google Scholar  * Greenwald, I. & Rubin, G. M. _Cell_ 68,

271–281 (1992). Article  CAS  PubMed  Google Scholar  * Wharton, K. A., Johansen, K. M., Xu, T. & Artavanis-Tsakonas, S. _Cell_ 43, 567–581 (1985). Article  CAS  PubMed  Google Scholar 

* Kidd, S., Kelley, M. R. & Young, M. W. _Molec. cell. Biol._ 6, 3094–3108 (1986). Article  CAS  PubMed  PubMed Central  Google Scholar  * Coffman, C., Harris, W. & Kintner, C.

_Science_ 249, 1438–1441 (1990). Article  ADS  CAS  PubMed  Google Scholar  * Weinmaster, G., Roberts, V. J. & Lemke, G. _Development_ 113, 199–205 (1991). CAS  PubMed  Google Scholar  *

Franco del Amo, F. _et al._ _Development_ 115, 737–744 (1992). CAS  Google Scholar  * Ellisen, L. W. _et al._ _Cell_ 66, 649–661 (1991). Article  CAS  PubMed  Google Scholar  * Stifani, S.,

Blaumueller, C. M., Redhead, N. J., Hill, R. E. & Artavanis-Tsakonas, S. _Nature Genet._ 2, 119–127 (1992). Article  CAS  PubMed  Google Scholar  * Rebay, I., Fehon, R. G. &

Artavanis-Tsakonas, S. _Cell_ 74, 319–329 (1993). Article  CAS  PubMed  Google Scholar  * Robbins, J., Blondel, B. J., Gallahan, D. & Callahan, R. _J. Virol._ 66, 2594–2599 (1992). CAS 

PubMed  PubMed Central  Google Scholar  * Jhappan, C. _et al._ _Genes Dev._ 6, 345–355 (1992). Article  CAS  PubMed  Google Scholar  * Coffman, C. R., Skoglund, P., Harris, W. A. &

Kintner, C. R. _Cell_ 73, 659–671 (1993). Article  CAS  PubMed  Google Scholar  * Tomlinson, A., Bowtell, D. D. L., Hafen, E. & Rubin, G. M. _Cell_ 51, 143–150 (1987). Article  CAS 

PubMed  Google Scholar  * Bowtell, D. D. L., Kimmel, B. E., Simon, M. A. & Rubin, G. M. _Proc. natn. Acad. Sci. U.S.A._ 86, 6245–6249 (1989). Article  ADS  CAS  Google Scholar  * Fehon,

R. G., Johansen, K., Rebay, I. & Artavanis-Tsakonas, S. _J. Cell Biol._ 113, 657–669 (1991). Article  CAS  PubMed  Google Scholar  * Heitzler, P. & Simpson, P. _Cell_ 64, 1083–1092

(1991). Article  CAS  PubMed  Google Scholar  * Mlodzik, M., Hiromi, Y., Weber, U., Goodman, C. S. & Rubin, G. M. _Cell_ 60, 211–224 (1990). Article  CAS  PubMed  Google Scholar  *

Heberlein, U., Mlodzik, M. & Rubin, G. M. _Development_ 112, 703–712 (1991). CAS  PubMed  Google Scholar  * Rubin, G. M. _Trends Genet._ 7, 372–377 (1991). Article  CAS  PubMed  Google

Scholar  * Tomlinson, A. & Ready, D. F. _Devl Biol._ 123, 264–275 (1987). Article  CAS  Google Scholar  * Reinke, R. & Zipursky, S. L. _Cell_ 55, 321–330 (1988). Article  CAS  PubMed

  Google Scholar  * Carthew, R. W. & Rubin, G. M. _Cell_ 63, 561–577 (1990). Article  CAS  PubMed  Google Scholar  * Smoller, D. _et al._ _Genes Dev._ 4, 1688–1700 (1990). Article  CAS 

PubMed  Google Scholar  * Delidakis, C., Preiss, A., Hartley, D. A. & Artavanis-Tsakonas, S. _Genetics_ 129, 803–823 (1991). CAS  PubMed  PubMed Central  Google Scholar  * Bier, E.,

Ackerman, L., Barbel, S., Jan, L. & Jan, Y. N. _Science_ 240, 913–916 (1988). Article  ADS  CAS  PubMed  Google Scholar  * Robinow, S. & White, K. _J. Neurobiol._ 22, 443–461 (1991).

Article  CAS  PubMed  Google Scholar  * Fortini, M. E., Simon, M. A. & Rubin, G. M. _Nature_ 355, 559–561 (1992). Article  ADS  CAS  PubMed  Google Scholar  * Fortini, M. E. &

Rubin, G. M. _Genes Dev._ 4, 444–463 (1990). Article  CAS  PubMed  Google Scholar  * Gaul, U., Mardon, G. & Rubin, G. M. _Cell_ 68, 1007–1019 (1992). Article  CAS  PubMed  Google Scholar

  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departments of Cell Biology and Biology, and Howard Hughes Medical Institute, Yale University, New Haven, Connecticut,

06536, USA Mark E. Fortini, Ilaria Rebay, Laurent A. Caron & Spyros Artavanis-Tsakonas Authors * Mark E. Fortini View author publications You can also search for this author inPubMed 

Google Scholar * Ilaria Rebay View author publications You can also search for this author inPubMed Google Scholar * Laurent A. Caron View author publications You can also search for this

author inPubMed Google Scholar * Spyros Artavanis-Tsakonas View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Fortini, M., Rebay, I., Caron, L. _et al._ An activated Notch receptor blocks cell-fate commitment in the developing _Drosophila_ eye.

_Nature_ 365, 555–557 (1993). https://doi.org/10.1038/365555a0 Download citation * Received: 19 May 1993 * Accepted: 24 August 1993 * Issue Date: 07 October 1993 * DOI:

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