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We reported in this paper and elsewhere1 that mutants of the green alga Chlamydomonas reinhardtii that lacked detectable levels of functional photosystem I (PS I) are capable of
photoreduction of atmospheric CO2, autotrophic growth, and sustained simultaneous photoevolution of H2 and O2. Although the absence of PS I in mutants B4 and F8 has been confirmed by
physical, biochemical and genetic techniques, subsequent analyses in our own laboratories, as well as in those of colleagues to whom we have sent the mutants, indicate that there is
variability in the PS I content of the cultures depending on growth conditions. Although some strains retain undetectable levels of P700, others contain variable (0–20%) amounts of wild-type
P700. (See alsorefs 2,3).
The online version of the original article can be found at 10.1038/376438a0
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