ABSTRACT AIM: The renin-angiotensin system plays a crucial role in the development and establishment of hypertension, and the pharmacological blockade of the system results in a reduction in

blood pressure. In the present study, we investigated whether the effects of a novel, double-stranded, recombinant adeno-associated virus vector (rAAV)-mediated antisense angiotensin II

receptor 1 (AT1R) gene efficiently prevents the development of hypertension induced by a high-salt diet in adult, male Sprague-Dawley (SD) rats. METHODS: A rAAV was prepared with a cassette

containing a cytomegaloviruspromoter and partial cDNA (660 base pairs) for the AT1R inserted in the antisense direction (rAAV-AT1-AS). A single tail vein injection of the rAAV-AT1-AS or

however, the rAAV-AT1-AS treatment attenuated the rise in blood pressure (142.7±4.5 mmHg _vs_ 117±3.8 mmHg, _P_<0.01), and the hypotensive effect was maintained until the experiments

ended at 12 weeks. In the rAAV-GFP-treated animals AT1 was overexpressed in various tissues, especially in the aorta and kidney at mRNA levels; in contrast, rAAV-AT1-AS treatment markedly

attenuated AT1 expression. Further more, rAAV-AT1-AS treatment prevented target organ damages from hypertension, including cardiac dysfunction and renal injury compared to the rAAV-GFP

group. CONCLUSION: These results suggest that rAAV-mediated anti-AT1 delivery attenuates the development of hypertension and protects against renal injury and cardiac remodeling. SIMILAR

CONTENT BEING VIEWED BY OTHERS ENHANCEMENT OF ANGIOTENSIN II TYPE 1 RECEPTOR-ASSOCIATED PROTEIN IN THE PARAVENTRICULAR NUCLEUS SUPPRESSES ANGIOTENSIN II-DEPENDENT HYPERTENSION Article 26

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Author notes * Xu-guang Li and Jiang-tao Yan: These authors contributed equally to this study. AUTHORS AND AFFILIATIONS * Institute of Hypertension and Department of Internal Medicine,

Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China Xu-guang Li, Jiang-tao Yan, Xi-zheng Xu, Jia-ning Wang, Li-ming Cheng, Tao Wang, 

Ping Zuo & Dao-wen Wang Authors * Xu-guang Li View author publications You can also search for this author inPubMed Google Scholar * Jiang-tao Yan View author publications You can also

search for this author inPubMed Google Scholar * Xi-zheng Xu View author publications You can also search for this author inPubMed Google Scholar * Jia-ning Wang View author publications You

can also search for this author inPubMed Google Scholar * Li-ming Cheng View author publications You can also search for this author inPubMed Google Scholar * Tao Wang View author

publications You can also search for this author inPubMed Google Scholar * Ping Zuo View author publications You can also search for this author inPubMed Google Scholar * Dao-wen Wang View

author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Dao-wen Wang. ADDITIONAL INFORMATION This work was supported by grants

from the National 863 Plan project (No 2004AA-217060), the National Basic Research “973” Program (No 2006CB503801), and the National Education Ministration project (No 20040487079). RIGHTS

AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Li, Xg., Yan, Jt., Xu, Xz. _et al._ Recombinant adeno-associated virus-mediated delivery of antisense

angiotensin II receptor 1 gene attenuates hypertension development. _Acta Pharmacol Sin_ 28, 1737–1745 (2007). https://doi.org/10.1111/j.1745-7254.2007.00676.x Download citation * Received:

11 June 2007 * Accepted: 24 June 2007 * Issue Date: 01 November 2007 * DOI: https://doi.org/10.1111/j.1745-7254.2007.00676.x SHARE THIS ARTICLE Anyone you share the following link with will

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content-sharing initiative KEYWORDS * hypertension * gene therapy * angiotensin II receptor 1 * antisense * double-stranded recombinant adeno-associated virus vector