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ABSTRACT Hepatocyte suspensions were transplanted to the livers of syngeneic Wistar Kyoto rats by means of intraportal injection. Labelling of the donor cells with 51Cr or tritiated
thymidine showed that 20% of the cells survived the transplantation procedure and were permanently retained by the recipient liver. Hepatocytes transplanted from normal livers produced no
tumours, whereas donor cells from preneoplastic livers of rats treated with the carcinogens diethylnitrosamine and 2-acetylaminofluorene produced neoplastic nodules and hepatocellular
carcinomas in the recipients. The number of tumours per host liver was proportional to the number of hepatocytes transplanted. Treatment of the host rats with phenobarbitone accelerated
tumour development, causing liver cancer in the majority of the animals within three months. As opposed to the polyploid surrounding liver, both phenobarbitone-promoted and unpromoted host
tumours contained predominantly (70-90%) diploid cells, regardless of the wide range of transplant ploidies (10-80% diploid cells) achieved by means of centrifugal elutriation. The results
indicate that all host tumours arise from diploid donor hepatocytes and that the acquisition of a constitutive, predominantly non-polyploidising growth pattern may be a characteristic
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Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS HYPERPOLYPLOIDIZATION OF HEPATOCYTE INITIATES PRENEOPLASTIC LESION FORMATION IN THE LIVER Article Open access
28 January 2021 PROLIFERATIVE POLYPLOID CELLS GIVE RISE TO TUMORS VIA PLOIDY REDUCTION Article Open access 28 January 2021 HEPATOCYTE ORGANOIDS AND CELL TRANSPLANTATION: WHAT THE FUTURE
HOLDS Article Open access 18 October 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Tissue Culture, Institute for Cancer Research, Norwegian Radium Hospital, Oslo G Saeter
Authors * G Saeter View author publications You can also search for this author inPubMed Google Scholar * PE Schwarze View author publications You can also search for this author inPubMed
Google Scholar * JM Nesland View author publications You can also search for this author inPubMed Google Scholar * PO Seglen View author publications You can also search for this author
inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Saeter, G., Schwarze, P., Nesland, J. _et al._ Diploid nature of hepatocellular
tumours developing from transplanted preneoplastic liver cells. _Br J Cancer_ 59, 198–205 (1989). https://doi.org/10.1038/bjc.1989.41 Download citation * Issue Date: 01 February 1989 * DOI:
https://doi.org/10.1038/bjc.1989.41 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently
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