Diploid nature of hepatocellular tumours developing from transplanted preneoplastic liver cells

Diploid nature of hepatocellular tumours developing from transplanted preneoplastic liver cells

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ABSTRACT Hepatocyte suspensions were transplanted to the livers of syngeneic Wistar Kyoto rats by means of intraportal injection. Labelling of the donor cells with 51Cr or tritiated thymidine showed that 20% of the cells survived the transplantation procedure and were permanently retained by the recipient liver. Hepatocytes transplanted from normal livers produced no tumours, whereas donor cells from preneoplastic livers of rats treated with the carcinogens diethylnitrosamine and 2-acetylaminofluorene produced neoplastic nodules and hepatocellular carcinomas in the recipients. The number of tumours per host liver was proportional to the number of hepatocytes transplanted. Treatment of the host rats with phenobarbitone accelerated tumour development, causing liver cancer in the majority of the animals within three months. As opposed to the polyploid surrounding liver, both phenobarbitone-promoted and unpromoted host tumours contained predominantly (70-90%) diploid cells, regardless of the wide range of transplant ploidies (10-80% diploid cells) achieved by means of centrifugal elutriation. The results indicate that all host tumours arise from diploid donor hepatocytes and that the acquisition of a constitutive, predominantly non-polyploidising growth pattern may be a characteristic property of hepatocellular tumours. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 24 print issues and online access $259.00 per year only $10.79 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS HYPERPOLYPLOIDIZATION OF HEPATOCYTE INITIATES PRENEOPLASTIC LESION FORMATION IN THE LIVER Article Open access 28 January 2021 PROLIFERATIVE POLYPLOID CELLS GIVE RISE TO TUMORS VIA PLOIDY REDUCTION Article Open access 28 January 2021 HEPATOCYTE ORGANOIDS AND CELL TRANSPLANTATION: WHAT THE FUTURE HOLDS Article Open access 18 October 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Tissue Culture, Institute for Cancer Research, Norwegian Radium Hospital, Oslo G Saeter Authors * G Saeter View author publications You can also search for this author inPubMed Google Scholar * PE Schwarze View author publications You can also search for this author inPubMed Google Scholar * JM Nesland View author publications You can also search for this author inPubMed Google Scholar * PO Seglen View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Saeter, G., Schwarze, P., Nesland, J. _et al._ Diploid nature of hepatocellular tumours developing from transplanted preneoplastic liver cells. _Br J Cancer_ 59, 198–205 (1989). https://doi.org/10.1038/bjc.1989.41 Download citation * Issue Date: 01 February 1989 * DOI: https://doi.org/10.1038/bjc.1989.41 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT Hepatocyte suspensions were transplanted to the livers of syngeneic Wistar Kyoto rats by means of intraportal injection. Labelling of the donor cells with 51Cr or tritiated


thymidine showed that 20% of the cells survived the transplantation procedure and were permanently retained by the recipient liver. Hepatocytes transplanted from normal livers produced no


tumours, whereas donor cells from preneoplastic livers of rats treated with the carcinogens diethylnitrosamine and 2-acetylaminofluorene produced neoplastic nodules and hepatocellular


carcinomas in the recipients. The number of tumours per host liver was proportional to the number of hepatocytes transplanted. Treatment of the host rats with phenobarbitone accelerated


tumour development, causing liver cancer in the majority of the animals within three months. As opposed to the polyploid surrounding liver, both phenobarbitone-promoted and unpromoted host


tumours contained predominantly (70-90%) diploid cells, regardless of the wide range of transplant ploidies (10-80% diploid cells) achieved by means of centrifugal elutriation. The results


indicate that all host tumours arise from diploid donor hepatocytes and that the acquisition of a constitutive, predominantly non-polyploidising growth pattern may be a characteristic


property of hepatocellular tumours. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your


institution Subscribe to this journal Receive 24 print issues and online access $259.00 per year only $10.79 per issue Learn more Buy this article * Purchase on SpringerLink * Instant


access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions *


Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS HYPERPOLYPLOIDIZATION OF HEPATOCYTE INITIATES PRENEOPLASTIC LESION FORMATION IN THE LIVER Article Open access


28 January 2021 PROLIFERATIVE POLYPLOID CELLS GIVE RISE TO TUMORS VIA PLOIDY REDUCTION Article Open access 28 January 2021 HEPATOCYTE ORGANOIDS AND CELL TRANSPLANTATION: WHAT THE FUTURE


HOLDS Article Open access 18 October 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Tissue Culture, Institute for Cancer Research, Norwegian Radium Hospital, Oslo G Saeter


Authors * G Saeter View author publications You can also search for this author inPubMed Google Scholar * PE Schwarze View author publications You can also search for this author inPubMed 


Google Scholar * JM Nesland View author publications You can also search for this author inPubMed Google Scholar * PO Seglen View author publications You can also search for this author


inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Saeter, G., Schwarze, P., Nesland, J. _et al._ Diploid nature of hepatocellular


tumours developing from transplanted preneoplastic liver cells. _Br J Cancer_ 59, 198–205 (1989). https://doi.org/10.1038/bjc.1989.41 Download citation * Issue Date: 01 February 1989 * DOI:


https://doi.org/10.1038/bjc.1989.41 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently


available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative