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ABSTRACT Altered expression of the retinoblastoma (RB) tumour-suppressor gene product (pRB) has been detected in sporadic bone and soft-tissue sarcomas. Earlier studies, analysing small
cohorts of sarcoma patients, have suggested that these alterations are more commonly associated with high-grade tumours, metastatic lesions and poorer survival. This study was designed to
re-examine the prevalence and clinical significance of altered pRB expression in a large and selected group of soft-tissue sarcomas from 174 adult patients. Representative tissue sections
from these sarcomas were analysed by immunohistochemistry using a well-characterised anti-pRB monoclonal antibody. Tumours were considered to have a positive pRB phenotype only when pure
nuclear staining was demonstrated, and cases were segregated into one of three groups. Group 1 (n = 36) were patients whose tumours have minimal or undetectable pRB nuclear staining (<
20% of tumour cells) and were considered pRB negative. Patients with tumours staining in a heterogeneous pattern (20-79% of tumour cells) were classified as group 2 (n = 99). The staining of
group 3 (n = 39) was strongly positive with a homogeneous pRB nuclear immunoreactivity (80-100% of tumour cells). pRB alterations were frequently observed in both low- and high-grade
lesions. Altered pRB expression did not correlate with known predictors of survival and was not itself an independent predictor of outcome in the long-term follow-up. These findings support
earlier observations that alterations of pRB expression are common events in soft-tissue sarcomas; nevertheless, long-term follow-up results indicate that altered patterns of pRB expression
do not influence clinical outcome of patients affected with soft-tissue sarcomas. It is postulated that RB alterations are primary events in human sarcomas and may be involved in
tumorigenesis or early phases of tumour progression in these neoplasias. Access through your institution Buy or subscribe This is a preview of subscription content, access via your
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* Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS CLINICAL SEQUENCING OF SOFT TISSUE AND BONE SARCOMAS DELINEATES
DIVERSE GENOMIC LANDSCAPES AND POTENTIAL THERAPEUTIC TARGETS Article Open access 15 June 2022 CLINICAL GENOMIC PROFILING IN THE MANAGEMENT OF PATIENTS WITH SOFT TISSUE AND BONE SARCOMA
Article Open access 15 June 2022 PREDICTIVE AND PROGNOSTIC TRANSCRIPTOMIC BIOMARKERS IN SOFT TISSUE SARCOMAS Article Open access 05 March 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *
Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA MS Karpeh Authors * MS Karpeh View author publications You can also search for this author inPubMed Google
Scholar * MF Brennan View author publications You can also search for this author inPubMed Google Scholar * WG Cance View author publications You can also search for this author inPubMed
Google Scholar * JM Woodruff View author publications You can also search for this author inPubMed Google Scholar * D Pollack View author publications You can also search for this author
inPubMed Google Scholar * ES Casper View author publications You can also search for this author inPubMed Google Scholar * ME Dudas View author publications You can also search for this
author inPubMed Google Scholar * E Latres View author publications You can also search for this author inPubMed Google Scholar * M Drobnjak View author publications You can also search for
this author inPubMed Google Scholar * C Cordon-Cardo View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions
ABOUT THIS ARTICLE CITE THIS ARTICLE Karpeh, M., Brennan, M., Cance, W. _et al._ Altered patterns of retinoblastoma gene product expression in adult soft-tissue sarcomas. _Br J Cancer_ 72,
986–991 (1995). https://doi.org/10.1038/bjc.1995.447 Download citation * Issue Date: 01 October 1995 * DOI: https://doi.org/10.1038/bjc.1995.447 SHARE THIS ARTICLE Anyone you share the
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