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CORRECTION TO: _British Journal of Cancer_ (2011) 105, 854–863. doi:10.1038/bjc.2011.270 Upon publication of this paper in Volume 105, the authors noticed an error in the ‘Materials and
Methods’ section, under the sub-heading ‘RNA isolation’. An incorrect primer sequence was introduced; we have, therefore, presented the full, corrected paragraph, below. RNA ISOLATION
High-quality total RNA was extracted from the abovementioned cell lines and tumour samples using TRIzol Reagent (Invitrogen, Breda, The Netherlands) according to the manufacturer's
instructions. Samples were pretreated with DNase I, checked for residual DNA contamination by PCR, after which cDNA synthesis was performed as described before (Looijenga _et al_, 2006; de
Jong _et al_, 2008a). For each sample, a no-reverse transcription control was used, and _HPRT_ was used as reference level of expression. Quantitative PCR was performed using the Real-Time
PCR HT7900 (Applied Biosystems, Foster City, CA, USA). Sequences for the OCT3/4 splice variant specific primers were as described before (Atlasi _et al_, 2008; de Jong _et al_, 2008a). These
are highly specific for the different isoforms and even discriminate between OCT4A and its pseudogenes. The following forward (—F) and reverse (—R) primers were used (annotation between
brackets=annotation from (Atlasi _et al_, 2008)): HPRT: HPRT244-exon2-F, 5′-AATTATGGACAGGACTGAACGTC-3′; HPRT243-exon3-R, 5′-CGTGGGGTCCTTTTCACCAGCAAG-3′. OCT4A: OCT4A-F (OCT4-AF)
5′-CTTCTCGCCCCCTCCAGGT-3′; OCT4A-R (OCT4-RB1) 5′-AAATAGAACCCCCAGGGTGAGC-3′. OCT4B: OCT4B-F (OCT4-FB) 5′-AGACTATTCCTTGGGGCCACAC-3′; OCT4B-R (OCT4-RB5) 5′-GGCTGAATACCTTCCCAAATAGA-3′. OCT4B1:
OCT4B-F (OCT4-FB), 5′-AGACTATTCCTTGGGGCCACAC-3′; OCT4B1-R (OCT-RB4) 5′-CTTAGAGGGGAGATGCGGTCA-3′. The localisation of the different primers is depicted in Figure 1. The efficiency and
specificity of these primers was extensively tested before (Atlasi _et al_, 2008). The specificity for human RNA is proven by the absence of any _OCT4_A/B/B1 expression in most of the
xenografts, specifically in PC82, which has a large stromal component. Quantitative values were obtained from the Ct. _OCT3/4_ mRNAs (A, B and B1) were quantified with relative to _HPRT_
(_OCT3/4_ mRNA=2(mean Ct HPRT−mean Ct OCT3/4 (A, B or B1))) as described before (Livak and Schmittgen, 2001). The _OCT4_B1 PCR products were sequenced using OCT4B1-F and a primer in exon 5
(OCT4B1-R2: (OCT4-RB3) 5′-CCCCCTGTCCCCCATTCCTA-3′) to verify the nature of this splice variant. MicroRNA expression was measured as described previously (Gillis _et al_, 2007). CHANGE
HISTORY * _ 29 MARCH 2012 This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication _ Authors * M A Rijlaarsdam View
author publications You can also search for this author inPubMed Google Scholar * H A D M van Herk View author publications You can also search for this author inPubMed Google Scholar * A J
M Gillis View author publications You can also search for this author inPubMed Google Scholar * H Stoop View author publications You can also search for this author inPubMed Google Scholar *
G Jenster View author publications You can also search for this author inPubMed Google Scholar * J Martens View author publications You can also search for this author inPubMed Google
Scholar * G J L H van Leenders View author publications You can also search for this author inPubMed Google Scholar * W Dinjens View author publications You can also search for this author
inPubMed Google Scholar * A M Hoogland View author publications You can also search for this author inPubMed Google Scholar * M Timmermans View author publications You can also search for
this author inPubMed Google Scholar * L H J Looijenga View author publications You can also search for this author inPubMed Google Scholar ADDITIONAL INFORMATION The online version of the
original article can be found at 10.1038/bjc.2011.270 RIGHTS AND PERMISSIONS From twelve months after its original publication, this work is licensed under the Creative Commons
Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ Reprints and permissions ABOUT THIS
ARTICLE CITE THIS ARTICLE Rijlaarsdam, M., van Herk, H., Gillis, A. _et al._ Erratum: Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines:
confirmation of OCT3/4 specificity for germ cell tumours. _Br J Cancer_ 106, 791 (2012). https://doi.org/10.1038/bjc.2012.37 Download citation * Published: 14 February 2012 * Issue Date: 14
February 2012 * DOI: https://doi.org/10.1038/bjc.2012.37 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable
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