ABSTRACT Apoptosis is induced by various stresses generated from the extracellular and intracellular environments. The fidelity of the cell cycle is monitored by surveillance mechanisms that

arrest its further progression if any crucial process has not been completed or damages are sustained, and then the cells with problems undergo apoptosis. Although the molecular mechanisms

involved in the regulation of the cell cycle and that of apoptosis have been elucidated, the links between them are not clear, especially that between cell cycle and death receptor-mediated

apoptosis. By using the HeLa.S-Fucci (fluorescent ubiquitination-based cell cycle indicator) cells, we investigated the relationship between the cell cycle progression and apoptotic

culture of the cells. These results were confirmed by using synchronized culture of the cells. Furthermore, mitotic cells showed the resistance to Fas-mediated apoptosis. In conclusion,

these findings suggest that apoptotic execution is dependent on cell cycle phase and Fas-mediated apoptosis preferentially occurs at G1 phase. SIMILAR CONTENT BEING VIEWED BY OTHERS THE

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Apoptosis is a mechanism of cell death that is fundamental in many biological phenomena, including morphogenesis and maintenance of tissue homeostasis. Apoptosis is characterized by

chromatin condensation, nuclear fragmentation, and formation of membrane-enclosed vesicles called apoptotic bodies, which are phagocytosed by other cells. Various stresses generated from the

extracellular and intracellular environments induce apoptosis. Stimuli that trigger apoptosis in mammalian cells can be summarized into two major categories of apoptotic pathways: the

extrinsic and intrinsic pathways.1, 2, 3 The extrinsic pathway occurs in response to external signals. This is also referred to as the death receptor pathway, as it is mediated by ligation

of cell surface death receptors to their cognate ligands. Ligation of death receptors induces subsequent downstream signaling through the initiator caspase-8 and/or caspase-10. Intrinsic

apoptotic signaling occurs in response to stimuli such as DNA damage, growth factor withdrawal, and exposure to certain chemotherapeutic agents, all of which result in the release of

cytochrome _c_ and other pro-death factors from the intermembrane space of the mitochondria and subsequent downstream signaling through the initiator caspase-9. Fas/CD95 is a member of the

tumor necrosis factor receptor superfamily, and induces apoptosis through the extrinsic pathway. The activation of Fas/CD95 by its specific ligand, FasL/CD95L, induces apoptosis in

susceptible target cells.4 After activation of Fas/CD95, the adapter protein, Fas-associated protein with death domain (FADD), binds to the death domain of Fas/CD95 and attracts procaspase-8

via its death effector domain to the receptor complex, forming the death-inducing signaling complex (DISC).5, 6, 7 Upon DISC formation, procaspase-8 is autolytically cleaved and activated

and, in turn, cleaves downstream caspases such as effector caspase-3 and -7, leading to cleavage of cellular proteins and DNA and to subsequence apoptotic cell death.8 On the other hand,

etoposide (VP-16) is one of the most widely used anticancer drugs, belonging to the family of DNA topoisomerase II inhibitors. Etoposide causes DNA double-strand breaks through the formation

of a cleavage complex containing DNA–drug–enzyme, and induces apoptosis through the intrinsic pathway.9 Cell cycle checkpoints restrain further cell cycle progression if a process has not

been successfully completed or DNA damage has been sustained.10 Checkpoints operate to prevent further DNA replication within S phase when the replication complexes are stalled, to prevent

entry into mitosis when DNA replication is not completed, and to prevent chromosome segregation when mitotic spindle assembly has not been completed. DNA damage-induced checkpoints also

inhibit entry into S phase, progression through S phase, and entry into mitosis. Recently, HeLa.S-Fucci (fluorescent ubiquitination-based cell cycle indicator) cells were established by

Miyawaki group,11 which express monomeric Kusabira-Orange 2 (mKO2) and monomeric Azami-Green 1 (mAG1) fused to the ubiquitination domains of Cdt1 and geminin, respectively, to monitor the

cell cycle progression _in situ_. As Cdt1 and geminin are the direct substrates of SCFSkp2 and APC/CCdh1 complexes, respectively, the level of Cdt1 is highest at G1 phase whereas geminin is

prominent during S, G2, and M phases. Therefore, the cell nuclei of HeLa.S-Fucci cells during the cell cycle are labeled with orange of mKO2 fused to the ubiquitination domain of Cdt1 in G1

phase and green of mAG1 fused to the ubiquitination domain of geminin in S, G2, and M phases. The availability of the Fucci system for the analysis of tumor biology and cell biology in

relation to cell cycle regulation has been reported.12, 13, 14, 15, 16 It has been unclear whether the apoptotic events are dependent on the cell cycle phase, because the available methods

are not sufficient to monitor the apoptotic execution _in situ_. Common protocols such as immunoblotting and flow cytometry have been applied to examine the cell cycle progression and

apoptotic events, which indirectly indicated the cell cycle phase of apoptotic cells by using marker proteins such as cyclins and Rb, and DNA contents.17, 18, 19, 20, 21, 22, 23, 24 However,

cyclin E, Rb, and cyclin-dependent kinase (CDK) inhibitors are substrates for caspases,25, 26, 27, 28 and DNA contents gradually decrease during apoptotic execution. Furthermore, if the

cells were examined after synchronization using some drugs that are toxic to cells, it is difficult to distinguish whether apoptosis is induced by apoptotic stimuli. Here we used

HeLa.S-Fucci cells to directly monitor the cell cycle progression and determine the cell cycle phase of apoptotic cells induced by the extrinsic and intrinsic pathways under the time-lapse

fluorescent microscopy. Our results clearly indicated that apoptotic execution is dependent on cell cycle phase and Fas-mediated apoptosis preferentially occurs at G1 phase. RESULTS THE

RATIO OF CELL CYCLE PHASE OF NORMAL CELLS AFTER APOPTOSIS INDUCTION The relationship between apoptosis and the cell cycle remains unclear. Therefore, we set out to analyze the cell cycle

specificity of Fas-mediated and etoposide-induced apoptotic execution using HeLa.S-Fucci cells. First, we checked the sensitivities of HeLa.S-Fucci cells to the treatment of an agonistic

anti-Fas antibody and etoposide. The viability of the cells treated with the anti-Fas antibody decreased in a time-dependent manner accompanying the activation of caspases and the cleavage

of caspase substrate (Figures 1a and b). The response to etoposide had a time lag of about 12 h for the apoptosis execution and caspase activation (Figures 1c and d). To study the effects of

the agonistic anti-Fas antibody and etoposide on the cell cycle progression, we observed HeLa.S-Fucci cells with immunofluorescent microscope at 24 h after treatment (Figure 2a). Total

number of living cells, which attached on the culture dish and showed spread shape, decreased as compared with that of non-treated cells. Although the ratio of the cells having red nuclei to

the cells having green nuclei in non-treated and the anti-Fas antibody-treated cells seemed to be unchanged, the relative number of normal cells having green nuclei significantly increased

after treatment with etoposide, suggesting that etoposide-induced apoptosis occurred in a cell cycle-dependent manner. To confirm this, cells showing normal morphology were counted and

classified into each cell cycle phase according to the criteria as shown in Figure 2b. Cells treated with etoposide were significantly accumulated at S/G2/M phases with nuclei labeled with

green color, consistent with the pharmacological action of etoposide that inhibits topoisomerase II, thereby arrests the cells at S and G2 phases (Figure 2c). Additionally, we noticed a

slight decrease of cells at G1 phase accompanying an increase of cells at S/G2/M phases in the anti-Fas antibody-treated cells, suggesting that the Fas-mediated apoptosis preferentially

occurs at G1 phase. TIME-LAPSE OBSERVATION OF FAS-MEDIATED OR ETOPOSIDE-INDUCED APOPTOSIS To monitor the apoptotic execution during cell cycle progression, we observed HeLa.S-Fucci cells for

24 h after treatment without or with the agonistic anti-Fas antibody or etoposide with time-lapse fluorescent microscopy (see Supplementary Movies S1–S3), and the typical features of the

cells at 6 h after treatment with the anti-Fas antibody (Figure 3a) and at 24 h after treatment with etoposide (Figure 3b) were shown. Apoptotic cells with red color were marked with red

arrows, apoptotic cells with green color were with green arrows, apoptotic cells with both red and green colors were with yellow arrows, and apoptotic cells without any color were with white

arrows. Although most of the cells treated with etoposide had green color, suggesting that apoptotic cells were at S, G2, and M phases (Figure 3b), the majority of Fas-mediated apoptotic

cells had red color indicating that the cells were dead at G1 phase (Figure 3a). To confirm this, we counted apoptotic cells and classified into each cell cycle phase according to the

criteria as shown in Figure 4a. About 70% of apoptotic cells were present at G1 phase at 6 h after treatment with the anti-Fas antibody, and gradually decreased the apoptotic cells at G1

phase accompanying the increase of apoptotic cells at M/G1 phase (Figure 4b), suggesting that Fas-mediated apoptosis preferentially occurred at G1 phase. On the other hand, more than 60% of

etoposide-induced apoptotic cells had green color at 24 h after treatment with etoposide (Figure 4c), indicating that the etoposide-induced apoptotic execution occurred at S/G2/M phases.

PREFERENTIAL FAS-MEDIATED APOPTOSIS AT G1 PHASE IN SYNCHRONIZED CELLS To further examine the cell cycle-dependent apoptotic execution, synchronized cells were monitored under the time-lapse

fluorescent microscope (Figure 5 and Supplementary Movies S4–S6) and the numbers of normal, apoptotic, and mitotic cells were counted on the photographs from each movie. Synchronized

HeLa.S-Fucci cells at the border of G1 and S phases transited through M phase from 7 to 11 h after the release to enter S phase regardless of the presence or absence of the agonistic

anti-Fas antibody (Figure 5a), and the total number of cells increased (Figure 5b), suggesting that the treatment of the anti-Fas antibody did not interfere with the cell cycle progression

from S to M phases. However, no mitotic cells were observed in etoposide-treated synchronized cells (Figure 5a), total number of the cells gradually decreased (Figure 5b), and a large

fraction of the cells had nuclei labeling with green color (Supplementary Movie S6), suggesting that etoposide-treated cells arrested at S and/or G2 phases. The viability of synchronized

cells treated with the anti-Fas antibody decreased in a time-dependent manner, but the prominent decrease was observed between 12 and 15 h after the release to enter the cell cycle (Figure

5c), indicating that the sensitivities of the cells to the treatment with the anti-Fas antibody were transiently enhanced after M phase. To confirm these results, the number of apoptotic

cells per unit area was counted at each time point (Figure 5d), and the increase of apoptotic cells at each period was shown by the histogram (Figure 5e). Apoptotic cells appeared at the

highest level between 12 and 15 h, suggesting that the Fas-mediated apoptosis was preferentially executed at G1 phase. In contrast, the onset of apoptotic execution of etoposide-treated

cells was around 15 h after treatment, and thereafter the decrease of viability and the increase of apoptotic cells were observed in a time-dependent manner (Figures 5f–h). These results

suggested that apoptotic execution is dependent on cell cycle phase and Fas-mediated apoptosis preferentially occurs at G1 phase. RESISTANCE OF MITOTIC CELLS TO FAS-MEDIATED APOPTOSIS As

shown in Figures 5a and e, Fas-mediated apoptosis was transiently enhanced immediately after M phase. Furthermore, we noticed that there were no occurrence of apoptosis during M phase under

time-lapse fluorescent microscope observation of both asynchronous and synchronous cells after induction of Fas-mediated apoptosis (Supplementary Movies S2 and S5). These results suggest

that mitotic cells are resistant to Fas-mediated apoptosis. To confirm this, we counted mitotic cells on the movies and the images constructing the movies, and classified into normal and

dead cells (Table 1). Cells treated with the agonistic anti-Fas antibody normally transited through M phase, which have more than 95% viability throughout the observation, even though the

total cell viability was gradually decreased. These results strongly suggested that Fas-mediated apoptosis is not executed during M phase. FADD, a component of DISC, was suggested to be

phosphorylated at Ser-194 in arrested cells at G2/M phases.29 Therefore, we analyzed whether FADD is phosphorylated at Ser-194 during M phase (Figure 6). After synchronization of

HeLa.S-Fucci cells at the border of G1 and S phases with a double-thymidine block following its release into the cell cycle, cells were collected at each time point and analyzed by

immunoblotting. The doublet bands of FADD were detected at 8 and 10 h after release from the thymidine block. Furthermore, the band corresponding to phosphorylated FADD was detected with the

antibody specifically recognized phosphorylated FADD at Ser-194 at the same periods. As synchronized HeLa.S-Fucci cells at the border of G1 and S phases transited through M phase from 7 to

11 h after the release to enter S phase as described in Figure 5, these results suggested that FADD is phosphorylated at Ser-194 during M phase. DISCUSSION The relationship between the

apoptotic execution and the cell cycle progression had been difficult to be analyzed, because it was hard to determine the cell cycle phase of each apoptotic cell. Here we determined the

cell cycle phase of each apoptotic cell by using HeLa.S-Fucci cells under time-lapse fluorescent microscope observation, and showed that Fas-mediated apoptosis occurred preferentially at G1

phase whereas etoposide-induced apoptosis did at S and G2 phases. It was suggested that the cells treated with etoposide arrested at S and/or G2 phases, as most normal and apoptotic cells

had nuclei labeled with green color and no mitotic cells were observed (Figures 2, 3, 4, and Supplementary Movies S3 and S6). As etoposide forms a complex with topoisomerase II and DNA to

enhance double-strand and single-strand breaks and reversible inhibition of DNA religation, the cell cycle was arrested in S and/or G2 phases by the mechanisms of the cell cycle checkpoints,

leading to apoptotic cell death through the intrinsic pathway before the entry into M phase. Fas-mediated apoptosis was executed through the extrinsic pathway of apoptosis. Although several

groups tried to determine the cell cycle dependency of Fas-mediated apoptosis, the results were inconsistent: more sensitive at G1 phase,17, 21 no cell cycle dependency,18, 19 susceptible

at S phase,20, 30 and dependency at G1 and S phases.22, 23 Here we clearly indicated that Fas-mediated apoptosis occurred preferentially at G1 phase. Furthermore, we noticed that no

apoptosis occurred at M phase and the ratio of apoptotic cells which died immediately after cytokinesis increased in a time-dependent manner (Figure 4b and Supplementary Movies S2 and S5).

These results suggest that mitotic cells show a resistance to Fas-mediated apoptosis. How are mitotic cells resistant to Fas-mediated apoptosis? During mitosis, cells undergo dramatic

changes including chromosomal condensation, nuclear envelope breakdown, and mitotic spindle formation. Phosphorylation events of multiple proteins by CDKs and other mitotic kinases are

essential for the regulation of these processes.31, 32 The components of DISC were suggested to be phosphorylated during mitosis. FADD is the key adapter protein transmitting apoptotic

signals mediated by the death receptors.5, 7 FADD is also implicated in cell proliferation, cell cycle progression, tumor development, inflammation, innate immunity, and autophagy.33 Ser-194

of the FADD protein has been shown to undergo phosphorylation in cells arrested in G2/M phases, and three kinases able to phosphorylate FADD at the Ser-194 residue have been identified: the

37-kDa casein kinase I_α_, the 130-kDa Fas/FADD-interacting serine/threonine kinase (FIST-HIPK3), and Polo-like kinase 1.29, 34, 35, 36 Furthermore, the phosphorylation at Ser-203 of FADD

by mitotic kinase Aurora-A led to cooperative phosphorylation at Ser-194 of FADD.36 In contrast to pro-apoptotic cytoplasmic FADD, the phosphorylation at Ser-194 is suggested to be essential

for the nuclear localization of FADD,37 and nuclear FADD is implicated in survival mechanisms.38 As we detected the phosphorylation at Ser-194 of FADD during M phase (Figure 6), the

molecular mechanisms of the resistance to Fas-mediated apoptosis in mitotic cells may be partially explained by the phosphorylation at Ser-194 of FADD that sequestered FADD molecule in

nuclei thereby preventing DISC formation in the cytoplasm. Furthermore, phosphorylation at Ser-18 and Ser-21 of FADD during mitosis were identified by phosphoproteomics analysis.39 It will

be necessary to evaluate the possible contribution of these phosphorylation of FADD to the regulation of Fas-mediated apoptosis. On the other hand, it was recently reported that procaspase-8

was phosphorylated in mitotic cells by Cdk1/cyclin B1 on Ser-387, which prevented Fas-mediated apoptosis.40 However, as the phosphorylated procaspase-8 at Ser-387 was predominantly

localized at centrosomes from prometaphase to anaphase, further investigation will be needed to clear the relationship between the phosphorylation of procaspase-8 at Ser-387 and the

resistance of mitotic cells to Fas-mediated apoptosis. Additionally, several phosphorylation of the components of DISC during mitosis was reported by phosphoproteomics analysis, such as

Ser-209, Ser-212, Thr-214, and Thr-219 of Fas/CD95,41 Ser-276 and Ser-289 of procaspase-8.39 The physiological importance of the phosphorylation of these proteins in the DISC formation needs

to be clarified. MATERIALS AND METHODS CELL CULTURE, SYNCHRONIZATION, AND APOPTOSIS INDUCTION HeLa.S-Fucci cells11 were cultured in DMEM supplemented with 10% fetal bovine serum (FBS). For

synchronization at the border of G1 and S phases, cells were seeded at a density of 5 × 104 cells per 35-mm dish and cultured for 24 h. After exposure to 2.5 mM thymidine for 18 h, cells

were washed with phosphate-buffered saline (PBS) three times and incubated in fresh medium for 10 h, and then exposed to 2.5 mM thymidine again for 14 h. To release the cells from the

arrest, medium containing thymidine was removed, and the cells were washed with PBS three times and incubated in fresh medium. For induction of apoptosis, cells were treated with 0.5 _μ_g/ml

of the agonistic anti-Fas antibody (CH-11; Medical & Biological Laboratories, Nagoya, Japan) or 50 _μ_M etoposide (VP-16). IMMUNOBLOT ANALYSIS Cells were lysed in lysis buffer (20 mM

Tris-HCl (pH 7.5), 150 mM NaCl, 1% Nonidet P-40, 50 _μ_g/ml phenylmethanesulfonyl fluoride, 5 mM EDTA). Protein samples were separated by SDS–polyacrylamide gel electrophoresis and blotted

onto Immobilon polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). Each protein was detected using primary antibodies as indicated, horseradish peroxidase-conjugated

secondary antibodies, and ECL-plus detection reagent (GE Healthcare, Little Chalfont, UK). Anti-caspase-3 polyclonal antibody (9662), anti-caspase-8 polyclonal antibody (9764), anti-PARP

polyclonal antibody (9542), and anti-phospho-FADD (Ser-194) polyclonal antibody (2781) were obtained from Cell Signaling (Danvers, MA, USA); anti-caspase-9 monoclonal antibody (M054-3),

anti-FADD monoclonal antibody (M033-3), and cyclin B1 monoclonal antibody (K0128-3) were from Medical & Biological Laboratories; anti-caspase-7/MCH-3 monoclonal antibody (610812) was

from BD Biosciences (Rockville, MD, USA); anti-_α_-tubulin monoclonal antibody (T6074) was from Sigma (St. Louis, MO, USA); anti-cyclin E monoclonal antibody (sc-247) was from Santa Cruz

Biotechnology (Santa Cruz, CA, USA). TIME-LAPSE FLUORESCENCE MICROSCOPY For time-lapse fluorescence microscopy, HeLa.S-Fucci cells were plated on a 35-mm dish. The medium was replaced with

MEM supplemented with 10% FBS without phenol red, and dishes were placed in a humidified chamber at 37°C that was mounted on a fluorescence microscope (model BZ-8000; Keyence, Osaka, Japan)

with a constant supply of mixed air containing 5% CO2. For analyses with BZ-8000, cells were observed with a 20 X objective lens and image data were obtained automatically every 15 min from

seven different fields by using BZ-H1TL software (Keyence). In the original program by the manufacturer, cells are exposed to the intense mercury lamp light intermittently and the cells were

driven to apoptosis within the initial 6 h by the physical damage of the light. Therefore, we used the neutral density filters to reduce the light levels to 1.6% that allowed the cells to

proliferate during 48 h observation.42 ABBREVIATIONS * Fucci: fluorescent ubiquitination-based cell cycle indicator * FADD: Fas-associated protein with death domain * DISC: death-inducing

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ACKNOWLEDGEMENTS We thank Dr. Atsushi Miyawaki for HeLa.S-Fucci cells. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Areas of the Ministry of Education,

Culture, Sports, Science and Technology of Japan, and by Grant-in-Aid for Scientific Research (B) of Japan Society for the Promotion of Science. AUTHOR INFORMATION Author notes * T

Hashimoto and K Juso: These authors contributed equally to this work. AUTHORS AND AFFILIATIONS * Biosignal Research Center, Kobe University, Kobe, Japan T Hashimoto, K Juso, M Nakano, T

Nagano, S Kambayashi, A Nakashima, U Kikkawa & S Kamada * Department of Biology, Graduate School of Science, Kobe University, Kobe, Japan K Juso, M Nakano, T Nagano, S Kambayashi, U

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can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to S Kamada. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no conflict of interest.

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Fas-mediated apoptotic execution at G1 phase: the resistance of mitotic cells to the cell death. _Cell Death Dis_ 3, e313 (2012). https://doi.org/10.1038/cddis.2012.52 Download citation *

Received: 24 January 2012 * Revised: 29 March 2012 * Accepted: 13 April 2012 * Published: 24 May 2012 * Issue Date: May 2012 * DOI: https://doi.org/10.1038/cddis.2012.52 SHARE THIS ARTICLE

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by the Springer Nature SharedIt content-sharing initiative KEYWORDS * apoptosis * cell cycle * mitosis * Fas/CD95 * etoposide