Play all audios:
ABSTRACT In this study, the full lipL21 gene fragment encoding outer membrane protein LipL21 was cloned from _L. interrogans_ serovar Lai and inserted into eukaryotic expression vector
pcDNA3.1(+). The guinea pigs were immunized with pcDNA3.1(+)-lipL21, pcDNA3.1(+) or PBS. Six weeks after the second immunization, the splenocytes were isolated to detect their proliferative
ability by lymphocyte transformation experiments. In addition, microscopic agglutination test was used for quantitative detection of specific antibodies. The rest guinea pigs were challenged
intraperitoneally with _L. interogans_ sorevar Lai. Then, protective effect was evaluated on the basis of survival and histopathological lesions in the kidneys, lungs, and liver. The lipL21
gene was successfully expressed in COS-7 cells through recombinant pcDNA3.1(+)-lipL21. The titer of specific antibodies substantially increased, and the stimulation index of splenocytes
increased significantly. Hence, the pcDNA3.1(+)-lipL21 could protect the immunized guinea pigs from homotypic _Leptospira_ infection. Furthermore, no obvious pathologic changes were observed
in the pcDNA3.1(+)-lipL21 immunized guinea pigs. The results showed that the protective effect with pathogenic strains of _Leptospira_ was shared by LipL21 mediated through a plasmid
vector. Consequently, these results indicated that the lipL21 DNA vaccine was a promising candidate for the prevention of leptospirosis. Access through your institution Buy or subscribe This
is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 digital issues and online access to
articles $119.00 per year only $9.92 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which
are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS
IMMUNOGENICITY OF INTRAPERITONEAL AND INTRANASAL LIPOSOME ADJUVANTED VLP VACCINES AGAINST SARS-COV-2 INFECTION Article Open access 09 November 2024 A CANDIDATE SUBUNIT VACCINE INDUCES
PROTECTIVE IMMUNITY AGAINST _MYCOBACTERIUM AVIUM_ SUBSPECIES _PARATUBERCULOSIS_ IN MICE Article Open access 20 May 2023 A NEW GENERATION NEEDLE- AND ADJUVANT-FREE TRIVALENT PLAGUE VACCINE
UTILIZING ADENOVIRUS-5 NANOPARTICLE PLATFORM Article Open access 29 January 2021 AUTHOR INFORMATION Author notes * Hanjiang He and Wenyu Wang: These authors contributed equally to this work
AUTHORS AND AFFILIATIONS * Department of Parasitology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, 510080, China Hanjiang He, Zhongdao Wu & Zhiyue Lv * Key Laboratory
for Tropical Disease Control, Chinese Ministry of Education, Guangzhou, 510080, China Hanjiang He, Zhongdao Wu & Zhiyue Lv * Department of Pathogenic Biology, Xiangnan University,
Chenzhou, 423000, China Hanjiang He & Jun Li * Institute of Pathogenic Biology, Nanhua University, Hengyang, 421001, China Wenyu Wang & Lizhi Tan Authors * Hanjiang He View author
publications You can also search for this author inPubMed Google Scholar * Wenyu Wang View author publications You can also search for this author inPubMed Google Scholar * Zhongdao Wu View
author publications You can also search for this author inPubMed Google Scholar * Zhiyue Lv View author publications You can also search for this author inPubMed Google Scholar * Jun Li View
author publications You can also search for this author inPubMed Google Scholar * Lizhi Tan View author publications You can also search for this author inPubMed Google Scholar
CORRESPONDING AUTHOR Correspondence to Lizhi Tan. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE He, H., Wang, W., Wu, Z. _et al._ Protection of Guinea
Pigs against _Leptospira interrogans_ Serovar Lai by LipL21 DNA Vaccine. _Cell Mol Immunol_ 5, 385–391 (2008). https://doi.org/10.1038/cmi.2008.48 Download citation * Received: 19 February
2008 * Accepted: 28 September 2008 * Issue Date: 01 October 2008 * DOI: https://doi.org/10.1038/cmi.2008.48 SHARE THIS ARTICLE Anyone you share the following link with will be able to read
this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative
KEYWORDS * _Leptospira_ * lipL21 * DNA vaccine * immunoprotection