Efficient DNA damage sensing and repair is crucial to preserve genomic integrity and failure to detect or repair DNA breaks can cause mutations, contributing to the formation of tumors. One

key protein required for mediating DNA repair is the tumor suppressor 53BP1. Recent studies now demonstrate the crucial role of K48-linked ubiquitination and protein degradation for 53BP1

recruitment at sites of DNA damage. The linking of ubiquitin chains to proteins is a multistep process involving three different types of enzymes. First, the ubiquitin-activating enzyme E1

catalyzes the transfer of ubiquitin to the ubiquitin-conjugating enzyme E2, which finally conjugates the ubiquitin moeities to target proteins with E3 ubiquitin ligases. Ubiquitin possesses

protein-protein interactions or conformational changes. While the role of K63-linked ubiquitination in the DNA damage response has been demonstrated 1, the role of K48-polyubiquitin chains

and degradation remained unclear until recently. In the budding yeast _Saccharomyces cerevisiae_, components of the 19S and 20S proteasome are recruited to DNA breaks induced by the HO

endonuclease 2 and mutant strains lacking components of the proteasome displayed enhanced radio-sensitivity. In mammalian cells, inhibition of the proteasome causes defects in the

recruitment of multiple players of the DNA damage response pathway including phosphorylated ATM, 53BP1, NBS1, BRCA1, FANCD2 and RAD51 3. Furthermore, depletion of the proteasomal subunits

PSMB3 and PSMD4 inhibits the formation of FANCD2 foci following ionizing radiation 3. In addition, K48-linked polyubiquitin chains accumulate at sites of DNA damage, suggesting a role for

protein degradation during the DNA damage response 4. However, the molecular mechanisms controlling protein ubiquitination and degradation following DNA damage are still unclear. The recent

discovery of the ubiquitination cascade triggered by the RING finger E3 ubiquitin ligase RNF8 uncovers a novel pathway responsible for the recruitment of DNA damage effectors. In the

presence of DNA breaks, the PI3-like protein kinase ATM phosphorylates the histone variant H2AX on serine 139, creating a docking station for the mediator protein MDC1, which in turn

recruits the ubiquitin ligase RNF8 5,6,7. RNF8 then stimulates the K63-linked ubiquitination of histones H2A and H2AX neighboring the DNA break. The RNF168 and HERC2 ubiquitin ligases also

facilitate the linking of ubiquitin to H2A at damaged sites 8,9,10. The ubiquitination cascade mediated by RNF8, RNF168 and HERC2 is responsible for the efficient recruitment of RAP80/BRCA1

and 53BP1 to DNA damage foci. The formation of RAP80 foci is mediated by its ubiquitin-interacting motif (UIM), which binds to K63-linked ubiquitin chains associated to H2A. However, the

recruitment of 53BP1 does not seem to be dependent on K63-linked polyubiquitin chains. The tumor suppressor p53-binding protein 1 (53BP1) is an important mediator of the DNA damage response.

53BP1 is required for the efficient phosphorylation of CHK2 and activation of p53 after damage. Furthermore, 53BP1 contributes to DNA repair through non-homologous end-joining 11. The

formation of 53BP1 foci at DNA damage sites necessitates the binding of the dimethylated histone H4 on lysine 20 (H4K20) via its tandem tudor domain 12. Throughout the chromatin,

dimethylation of H4K20 is relatively abundant in untreated cells. However, 53BP1 foci are not observed in undamaged cells, suggesting a mechanism preventing the accessibility of dimethylated

H4K20. Nucleosome stacking may contribute to bury methylated H4K20, but since nucleosomes are highly dynamic structures, even buried sites become accessible for protein binding. It was

shown that the lysine methyltransferase MMSET is recruited to DNA breaks to locally increase the amount of methylated H4K20 13. However, the MMSET-mediated methylation of H4K20 or its

localization to DNA breaks is not dependent on RNF8 activity 13. Hence, the local enrichment of H4K20(me2) triggered by MMSET does not explain the requirement of RNF8 for the recruitment of

53BP1 5,6,7. It was initially thought that RNF8 only catalyzes K63-linked ubiquitination, but multiple recent reports describe the ability of RNF8 to stimulate the formation of K48-linked

ubiquitin chains 4,14,15, confirming a role for RNF8-mediated degradation or chromatin extraction during the DNA damage response. We identified the lysine demethylases JMJD2A and JMJD2B as

novel targets of RNF8-mediated K48-linked ubiquitination (Figure 1) 15. Both JMJD2A and JMJD2B possess a hybrid tandem tudor domain conferring the ability to bind methylated H4K20. In fact,

JMJD2A (KD = 2.0 μM) and JMJD2B (KD = 27.7 μM) bind dimethylated H4K20 with a higher relative affinity than 53BP1 (KD = 50.8 μM) and they can block the formation 53BP1 foci when ectopically

expressed 15. In response to DNA breaks, both RNF8 and RNF168 can directly ubiquitinate JMDJ2A, leading to its proteasomal degradation. Depletion of RNF8 in the cell caused marked defects in

the recruitment of 53BP1, but combined depletion of JMJD2A and JMJD2B in these cells restores the formation of 53BP1 foci 15. These results suggest that JMJD2A and JMJD2B are bound to

methylated H4K20 in untreated cells and that RNF8- and RNF168-dependent activity lead to their chromatin extraction and proteasomal degradation to allow the unmasking of H4K20 and binding of

53BP1. In addition to JMJD2A, novel substrates of RNF8-dependent degradation, such as KU80 and CHK2, have been recently identified, confirming the important role of proteasomal degradation

in the DNA damage response 14. K48-linked poly-ubiquitination also promotes the chromatin extraction of proteins by the ubiquitin-selective segregase VCP/p97. VCP/p97 is recruited to DNA

breaks in an RNF8-dependent fashion requiring the formation of K48-linked ubiquitin chains 4. The presence of VCP/p97 at DNA lesions is a prerequisite for 53BP1 foci formation and may

promote the chromatin extraction of ubiquitinated proteins 4. In fact, the Polycomb group protein L3MBTL1 is removed from DNA lesions by an RNF8- and VCP/p97-dependent mechanism 16. L3MBTL1

possess three MBT domains allowing its binding to methylated H4K20 and potentially competes with 53BP1 for this methylated histone mark at DNA damaged sites. The chromatin extraction of

ubiquitinated L3MBTL1 correlates with the local recruitment of 53BP1 16. The affinity of L3MBTL1 (KD = 211 μM 17) for dimethylated H4K20 is relatively low compared to 53BP1 (KD = 50.8 μM),

suggesting that JMJD2A (KD = 2.0 μM) and JMJD2B (KD = 27.7 μM) release from the chromatin is important for the formation of 53BP1 foci. The K48-linked ubiquitination of JMJD2A and JMJD2B by

RNF8 and RNF168 might also promote their VCP/p97-dependent chromatin extraction from DNA breaks as suggested by their rapid and massive dissociation from the chromatin 15. Together, these

recent reports elucidate the molecular mechanism underlying the recruitment of 53BP1 to DNA breaks and reconcile the previous models by unifying RNF8-mediated ubiquitination, proteasomal

degradation and the role of methylation of histone H4 on lysine 20. In conclusion, proteins possessing tandem tudor domains, such as JMJD2A, JMJD2B and the MBT-containing L3MBTL1, are

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Scholar  Download references ACKNOWLEDGEMENTS We thank Gillian Vogel and members of the Richard laboratory for helpful comments. FAM received postdoctoral fellowships from the Canadian

Institutes of Health Research (CIHR). This work was funded by CIHR grant MOP-67070 to SR. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Terry Fox Molecular Oncology Group and the Bloomfield

Center for Research on Aging, Sir Mortimer B Davis Jewish General Hospital, Segal Cancer Centre, Lady Davis Institute for Medical Research, 3755 Côte Ste-Catherine Road, Montréal, H3T 1E2,

Québec, Canada Frédérick A Mallette & Stéphane Richard * Departments of Medicine and Oncology, McGill University, Montréal, Québec, Canada Frédérick A Mallette & Stéphane Richard

Authors * Frédérick A Mallette View author publications You can also search for this author inPubMed Google Scholar * Stéphane Richard View author publications You can also search for this

author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Stéphane Richard. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Mallette, F.,

Richard, S. K48-linked ubiquitination and protein degradation regulate 53BP1 recruitment at DNA damage sites. _Cell Res_ 22, 1221–1223 (2012). https://doi.org/10.1038/cr.2012.58 Download

citation * Published: 10 April 2012 * Issue Date: August 2012 * DOI: https://doi.org/10.1038/cr.2012.58 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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