Molecular relationship between the a and b chromosomes of crepis capillaris

Molecular relationship between the a and b chromosomes of crepis capillaris

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ABSTRACT The molecular structure of the B chromosome of _Crepis capillaris_ has been analysed by DNA comparisons of plants with 2B and 0B chromosomes. No additional band, and no differences in the band intensity were detected in 2B plants after digestion with 18 restriction enzymes. One hundred and five clones were obtained from 2B plants using the phenol-emulsion reassociation technique in formamide (F-PERT). Only one (clone pCc21) gave rise to additional hybridization bands in the DNA from 2B plants, indicating that this sequence occurs both in A and B chromosomes. Clone pCc21 contains a 54-bp microsatellite sequence rich in CA dinucleotides, similar to those found in other eukaryotic genomes. We conclude that the B chromosome of _C. capillaris_ carries no specific highly repetitive sequence, but consists of sequences also present in the A chromosomes. The possibility remains that the heterochromatic pericentromeric region of the B has a different organization with respect to standard heterochromatin. SIMILAR CONTENT BEING VIEWED BY OTHERS CYTO-MOLECULAR CHARACTERIZATION OF RDNA AND CHROMATIN COMPOSITION IN THE NOR-ASSOCIATED SATELLITE IN CHESTNUT (_CASTANEA_ SPP.) Article Open access 15 January 2024 A CHROMOSOME-SCALE ASSEMBLY OF THE QUINOA GENOME PROVIDES INSIGHTS INTO THE STRUCTURE AND DYNAMICS OF ITS SUBGENOMES Article Open access 13 December 2023 MOLECULAR ORGANIZATION OF RECOMBINANT HUMAN-ARABIDOPSIS CHROMOSOMES IN HYBRID CELL LINES Article Open access 30 March 2021 ARTICLE PDF REFERENCES * Amos, A, and Dover, G. 1981. The distribution of repetitive DNAs between regular and supernumerary chromosomes in species of _Glossina_ (Tsetse): a two-step process in the origin of supernumeraries. _Chromosoma_, 81, 673–690. Article  CAS  Google Scholar  * Casna, N J, Novack, D F, Hsu, M T, and Ford, J P. 1986. Genomic analysis. II. Isolation of high molecular weight heteroduplex DNA following differential methylase protection and formamide-PERT hybridization. _Nucleic Acids Res_, 14, 7285–7303. Article  CAS  Google Scholar  * Chilton, M D, and McCarthy, B J. 1973. DNA from maize with and without B chromosomes: a comparative study. _Genetics_, 74, 605–614. CAS  PubMed  PubMed Central  Google Scholar  * Condit, R, and Hubbell, S P. 1991. Abundance and DNA sequence of two-base repeat regions in tropical tree genomes. _Genome_, 34, 66–71. Article  CAS  Google Scholar  * Dellaporta, S L, Wood, J, and Hicks, J B. 1983. A plant DNA minipreparation: version II. _Plant Mol Biol Rep_, 1, 19–21. Article  CAS  Google Scholar  * Dover, G A. 1976. Observations on the repeated sequence DNA of “A” and “B” chromosomes of genotypes in the Triticinae with contrasting patterns of meiotic chromosome pairing. _Chromosomes Today_, 5, 131–145. CAS  Google Scholar  * Eickbush, D G, Eickbush, T H, and Werren, J H. 1992. Molecular characterization of repetitive DNA sequences from a B chromosome. _Chromosoma_, 101, 575–583. Article  CAS  Google Scholar  * Flavell, R B. 1982. Chromosomal DNA sequences and their organization. In: Parthier, B. and Boulter, D. (eds) _Nucleic Acids and Proteins in Plants_, pp. 46–74. Springer, Berlin. Chapter  Google Scholar  * Hamada, H, Petrino, M G, and Kakunaga, T. 1982. A novel repeated element with Z-DNA forming potential is widely found in evolutionarily diverse eukaryotic genomes. _Proc Natl Acad Sci USA_, 79, 6465–6469. Article  CAS  Google Scholar  * Huijser, P, Henning, W, and Dukhof, R. 1987. Poly (dC-dA/dG-dT ) repeats in the _Drosophila_ genome. _Chromosoma_, 95, 209–215. Article  CAS  Google Scholar  * Jamilena, M, Ruiz Rejón, C, and Ruiz Rejón, M. 1993. Repetitive DNA sequence families in _Crepis capillaris_. _Chromosoma_, 102, 272–278. Article  CAS  Google Scholar  * Jamilena, M, Ruiz Rejón, C, and Ruiz Rejón, M. 1994. A molecular analysis of the origin of the _Crepis capillaris_ B chromosome. _J Cell Sei_, 107, 703–708. CAS  Google Scholar  * John, B. 1988. The biology of heterochromatin. In: Verma, R. S. (ed.) _Heterochromatin Molecular and Structural Aspects_, pp. 1–147. Cambridge University Press, Cambridge. Google Scholar  * John, B, Appels, R, and Contreras, N. 1986. Population cytogenetics of _Atractomorpha similis_. II. Molecular characterization of the distal C band polymorphisms. _Chromosoma_, 94, 45–58. Article  CAS  Google Scholar  * John, U P, Leach, C R, and Timmis, J N. 1991. A sequence specific to B chromosomes of _Brachycome dichromosomatica_. _Genome_, 34, 739–744. Article  CAS  Google Scholar  * Jones, R N, and Rees, H. 1982. _B Chromosomes_. Academic Press, London. Google Scholar  * Lima De Faria, A, Essen-Möller, J, Müntzing, A, Grandström, H, Olsson, E, and Isaksson, M. 1983. Comparison of DNA cleavage in rye with and without B chromosomes. _Hereditas_, 99, 203–208. Article  CAS  Google Scholar  * Lönn, M, Tegelström, H, and Prentice, H C. 1992. The synthetic (TG)_n_ polydinucleotide: a probe for gene flow and paternity studies in wild plant populations? _Nucleic Acids Res_, 20, 1153. Article  Google Scholar  * Maluszynska, J. 1990. B chromosomes of _Crepis capillaris_ (L.) Waller. _In vivo and in vitro_. Universytet Slaski, Katowice. Google Scholar  * Maluszynska, J, and Schweizer, D. 1989. Ribosomal RNA genes in B chromosomes of _Crepis capillaris_ detected by non-radioactive _in situ_ hybridization. _Heredity_, 62, 59–65. Article  Google Scholar  * Peacock, W J, Dennis, E S, Rhoades, M M, and Pryor, A J. 1981. Highly repeated DNA sequence limited to knob heterochromatin in maize. _Proc Natl Acad Sci USA_, 78, 4490–4494. Article  CAS  Google Scholar  * Rimpau, J, and Flavell, R B. 1976. The repeated sequence DNA of B chromosomes of rye. _Chromosomes Today_, 5, 147–157. CAS  Google Scholar  * Rogers, J. 1983. CACA sequences — the ends and the means? _Nature_, 305, 101–102. Article  CAS  Google Scholar  * Sandery, M J, Forster, J W, Blunden, R, and Jones, R N. 1990. Identification of a family of repeated sequences on the rye B chromosome. _Genome_, 33, 908–913. Article  CAS  Google Scholar  * Sanger, F, Nicklen, S, and Coulson, A R. 1977. DNA sequencing with chain terminating inhibitors. _Proc Natl Acad Sci USA_, 74, 5463–5467. Article  CAS  Google Scholar  * Tsujimoto, H, and Niwa, K. 1992. DNA structure of the B chromosome of rye revealed in _in situ_ hybridization using repetitive sequences. _Jap J Genet_, 67, 233–241. Article  Google Scholar  * Viotti, A, Privitera, E, Sala, E, and Pogna, N. 1985. Distribution and clustering of two highly repeated sequences in the A and B chromosomes of maize. _Theor Appl Genet_, 70, 234–239. Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departemento de Genética, Facultad de Ciencias, Universidad de Granada, Granada, 18071, Spain M Jamilena, M Garrido-Ramos, C Ruiz Rejón & M Ruiz Rejón Authors * M Jamilena View author publications You can also search for this author inPubMed Google Scholar * M Garrido-Ramos View author publications You can also search for this author inPubMed Google Scholar * C Ruiz Rejón View author publications You can also search for this author inPubMed Google Scholar * M Ruiz Rejón View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Jamilena, M., Garrido-Ramos, M., Ruiz Rejón, C. _et al._ Molecular relationship between the A and B chromosomes of _Crepis capillaris_. _Heredity_ 73, 527–531 (1994). https://doi.org/10.1038/hdy.1994.151 Download citation * Received: 18 March 1994 * Issue Date: 01 November 1994 * DOI: https://doi.org/10.1038/hdy.1994.151 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * B chromosomes * _Crepis capillaris_ * F-PERT * microsatellites

ABSTRACT The molecular structure of the B chromosome of _Crepis capillaris_ has been analysed by DNA comparisons of plants with 2B and 0B chromosomes. No additional band, and no differences


in the band intensity were detected in 2B plants after digestion with 18 restriction enzymes. One hundred and five clones were obtained from 2B plants using the phenol-emulsion reassociation


technique in formamide (F-PERT). Only one (clone pCc21) gave rise to additional hybridization bands in the DNA from 2B plants, indicating that this sequence occurs both in A and B


chromosomes. Clone pCc21 contains a 54-bp microsatellite sequence rich in CA dinucleotides, similar to those found in other eukaryotic genomes. We conclude that the B chromosome of _C.


capillaris_ carries no specific highly repetitive sequence, but consists of sequences also present in the A chromosomes. The possibility remains that the heterochromatic pericentromeric


region of the B has a different organization with respect to standard heterochromatin. SIMILAR CONTENT BEING VIEWED BY OTHERS CYTO-MOLECULAR CHARACTERIZATION OF RDNA AND CHROMATIN


COMPOSITION IN THE NOR-ASSOCIATED SATELLITE IN CHESTNUT (_CASTANEA_ SPP.) Article Open access 15 January 2024 A CHROMOSOME-SCALE ASSEMBLY OF THE QUINOA GENOME PROVIDES INSIGHTS INTO THE


STRUCTURE AND DYNAMICS OF ITS SUBGENOMES Article Open access 13 December 2023 MOLECULAR ORGANIZATION OF RECOMBINANT HUMAN-ARABIDOPSIS CHROMOSOMES IN HYBRID CELL LINES Article Open access 30


March 2021 ARTICLE PDF REFERENCES * Amos, A, and Dover, G. 1981. The distribution of repetitive DNAs between regular and supernumerary chromosomes in species of _Glossina_ (Tsetse): a


two-step process in the origin of supernumeraries. _Chromosoma_, 81, 673–690. Article  CAS  Google Scholar  * Casna, N J, Novack, D F, Hsu, M T, and Ford, J P. 1986. Genomic analysis. II.


Isolation of high molecular weight heteroduplex DNA following differential methylase protection and formamide-PERT hybridization. _Nucleic Acids Res_, 14, 7285–7303. Article  CAS  Google


Scholar  * Chilton, M D, and McCarthy, B J. 1973. DNA from maize with and without B chromosomes: a comparative study. _Genetics_, 74, 605–614. CAS  PubMed  PubMed Central  Google Scholar  *


Condit, R, and Hubbell, S P. 1991. Abundance and DNA sequence of two-base repeat regions in tropical tree genomes. _Genome_, 34, 66–71. Article  CAS  Google Scholar  * Dellaporta, S L, Wood,


J, and Hicks, J B. 1983. A plant DNA minipreparation: version II. _Plant Mol Biol Rep_, 1, 19–21. Article  CAS  Google Scholar  * Dover, G A. 1976. Observations on the repeated sequence DNA


of “A” and “B” chromosomes of genotypes in the Triticinae with contrasting patterns of meiotic chromosome pairing. _Chromosomes Today_, 5, 131–145. CAS  Google Scholar  * Eickbush, D G,


Eickbush, T H, and Werren, J H. 1992. Molecular characterization of repetitive DNA sequences from a B chromosome. _Chromosoma_, 101, 575–583. Article  CAS  Google Scholar  * Flavell, R B.


1982. Chromosomal DNA sequences and their organization. In: Parthier, B. and Boulter, D. (eds) _Nucleic Acids and Proteins in Plants_, pp. 46–74. Springer, Berlin. Chapter  Google Scholar  *


Hamada, H, Petrino, M G, and Kakunaga, T. 1982. A novel repeated element with Z-DNA forming potential is widely found in evolutionarily diverse eukaryotic genomes. _Proc Natl Acad Sci USA_,


79, 6465–6469. Article  CAS  Google Scholar  * Huijser, P, Henning, W, and Dukhof, R. 1987. Poly (dC-dA/dG-dT ) repeats in the _Drosophila_ genome. _Chromosoma_, 95, 209–215. Article  CAS 


Google Scholar  * Jamilena, M, Ruiz Rejón, C, and Ruiz Rejón, M. 1993. Repetitive DNA sequence families in _Crepis capillaris_. _Chromosoma_, 102, 272–278. Article  CAS  Google Scholar  *


Jamilena, M, Ruiz Rejón, C, and Ruiz Rejón, M. 1994. A molecular analysis of the origin of the _Crepis capillaris_ B chromosome. _J Cell Sei_, 107, 703–708. CAS  Google Scholar  * John, B.


1988. The biology of heterochromatin. In: Verma, R. S. (ed.) _Heterochromatin Molecular and Structural Aspects_, pp. 1–147. Cambridge University Press, Cambridge. Google Scholar  * John, B,


Appels, R, and Contreras, N. 1986. Population cytogenetics of _Atractomorpha similis_. II. Molecular characterization of the distal C band polymorphisms. _Chromosoma_, 94, 45–58. Article 


CAS  Google Scholar  * John, U P, Leach, C R, and Timmis, J N. 1991. A sequence specific to B chromosomes of _Brachycome dichromosomatica_. _Genome_, 34, 739–744. Article  CAS  Google


Scholar  * Jones, R N, and Rees, H. 1982. _B Chromosomes_. Academic Press, London. Google Scholar  * Lima De Faria, A, Essen-Möller, J, Müntzing, A, Grandström, H, Olsson, E, and Isaksson,


M. 1983. Comparison of DNA cleavage in rye with and without B chromosomes. _Hereditas_, 99, 203–208. Article  CAS  Google Scholar  * Lönn, M, Tegelström, H, and Prentice, H C. 1992. The


synthetic (TG)_n_ polydinucleotide: a probe for gene flow and paternity studies in wild plant populations? _Nucleic Acids Res_, 20, 1153. Article  Google Scholar  * Maluszynska, J. 1990. B


chromosomes of _Crepis capillaris_ (L.) Waller. _In vivo and in vitro_. Universytet Slaski, Katowice. Google Scholar  * Maluszynska, J, and Schweizer, D. 1989. Ribosomal RNA genes in B


chromosomes of _Crepis capillaris_ detected by non-radioactive _in situ_ hybridization. _Heredity_, 62, 59–65. Article  Google Scholar  * Peacock, W J, Dennis, E S, Rhoades, M M, and Pryor,


A J. 1981. Highly repeated DNA sequence limited to knob heterochromatin in maize. _Proc Natl Acad Sci USA_, 78, 4490–4494. Article  CAS  Google Scholar  * Rimpau, J, and Flavell, R B. 1976.


The repeated sequence DNA of B chromosomes of rye. _Chromosomes Today_, 5, 147–157. CAS  Google Scholar  * Rogers, J. 1983. CACA sequences — the ends and the means? _Nature_, 305, 101–102.


Article  CAS  Google Scholar  * Sandery, M J, Forster, J W, Blunden, R, and Jones, R N. 1990. Identification of a family of repeated sequences on the rye B chromosome. _Genome_, 33, 908–913.


Article  CAS  Google Scholar  * Sanger, F, Nicklen, S, and Coulson, A R. 1977. DNA sequencing with chain terminating inhibitors. _Proc Natl Acad Sci USA_, 74, 5463–5467. Article  CAS 


Google Scholar  * Tsujimoto, H, and Niwa, K. 1992. DNA structure of the B chromosome of rye revealed in _in situ_ hybridization using repetitive sequences. _Jap J Genet_, 67, 233–241.


Article  Google Scholar  * Viotti, A, Privitera, E, Sala, E, and Pogna, N. 1985. Distribution and clustering of two highly repeated sequences in the A and B chromosomes of maize. _Theor Appl


Genet_, 70, 234–239. Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departemento de Genética, Facultad de Ciencias, Universidad de Granada,


Granada, 18071, Spain M Jamilena, M Garrido-Ramos, C Ruiz Rejón & M Ruiz Rejón Authors * M Jamilena View author publications You can also search for this author inPubMed Google Scholar *


M Garrido-Ramos View author publications You can also search for this author inPubMed Google Scholar * C Ruiz Rejón View author publications You can also search for this author inPubMed 


Google Scholar * M Ruiz Rejón View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE


THIS ARTICLE Jamilena, M., Garrido-Ramos, M., Ruiz Rejón, C. _et al._ Molecular relationship between the A and B chromosomes of _Crepis capillaris_. _Heredity_ 73, 527–531 (1994).


https://doi.org/10.1038/hdy.1994.151 Download citation * Received: 18 March 1994 * Issue Date: 01 November 1994 * DOI: https://doi.org/10.1038/hdy.1994.151 SHARE THIS ARTICLE Anyone you


share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the


Springer Nature SharedIt content-sharing initiative KEYWORDS * B chromosomes * _Crepis capillaris_ * F-PERT * microsatellites