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ABSTRACT The molecular structure of the B chromosome of _Crepis capillaris_ has been analysed by DNA comparisons of plants with 2B and 0B chromosomes. No additional band, and no differences
in the band intensity were detected in 2B plants after digestion with 18 restriction enzymes. One hundred and five clones were obtained from 2B plants using the phenol-emulsion reassociation
technique in formamide (F-PERT). Only one (clone pCc21) gave rise to additional hybridization bands in the DNA from 2B plants, indicating that this sequence occurs both in A and B
chromosomes. Clone pCc21 contains a 54-bp microsatellite sequence rich in CA dinucleotides, similar to those found in other eukaryotic genomes. We conclude that the B chromosome of _C.
capillaris_ carries no specific highly repetitive sequence, but consists of sequences also present in the A chromosomes. The possibility remains that the heterochromatic pericentromeric
region of the B has a different organization with respect to standard heterochromatin. SIMILAR CONTENT BEING VIEWED BY OTHERS CYTO-MOLECULAR CHARACTERIZATION OF RDNA AND CHROMATIN
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Genet_, 70, 234–239. Article CAS Google Scholar Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departemento de Genética, Facultad de Ciencias, Universidad de Granada,
Granada, 18071, Spain M Jamilena, M Garrido-Ramos, C Ruiz Rejón & M Ruiz Rejón Authors * M Jamilena View author publications You can also search for this author inPubMed Google Scholar *
M Garrido-Ramos View author publications You can also search for this author inPubMed Google Scholar * C Ruiz Rejón View author publications You can also search for this author inPubMed
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THIS ARTICLE Jamilena, M., Garrido-Ramos, M., Ruiz Rejón, C. _et al._ Molecular relationship between the A and B chromosomes of _Crepis capillaris_. _Heredity_ 73, 527–531 (1994).
https://doi.org/10.1038/hdy.1994.151 Download citation * Received: 18 March 1994 * Issue Date: 01 November 1994 * DOI: https://doi.org/10.1038/hdy.1994.151 SHARE THIS ARTICLE Anyone you
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Springer Nature SharedIt content-sharing initiative KEYWORDS * B chromosomes * _Crepis capillaris_ * F-PERT * microsatellites