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Access through your institution Buy or subscribe Recent advances have been made towards successful generation of human hematopoietic stem and progenitor cells (HSPCs) derived from embryonic,
induced pluripotent or hematopoietic stem cells. However, whether these stem cell populations, which are generated after _ex vivo_ manipulation, are suitable for clinical application
depends on the faithful preservation of their transcriptional, epigenetic and functional properties with respect to their primary cell counterparts. To overcome the significant delay in
neutrophil recovery following cord blood (CB) transplantation (CBT), we developed methods for the _ex vivo_ expansion of CD34+ CB-derived HSPC by culture with the Notch-ligand Delta1. When
infused into patients undergoing a myeloablative CBT, these cells have been shown to provide more rapid short-term myeloid reconstitution when co-infused with a non-manipulated CB unit(s).
However, infusion of _ex vivo_ generated HSPCs for long-term hematopoietic reconstitution remains a goal in the context of stem cell transplantation and gene therapy, thus the development of
methods to assess the safety of these cultured products are essential. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution
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Stem Cell_ 2011; 8: 106–118. Article CAS Google Scholar Download references ACKNOWLEDGEMENTS This work was supported by National Heart, Lung and Blood Institute grant U01HL100395 and
National Institutes of Health Ruth L. Kirschstein National Research Service Award T32CA009351 (AD) and K12CA076930 (AD), as well as Hyundai Hope on Wheels 2013 Hope Grant (AD). CD is a Damon
Runyon Clinical Investigator. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Clinical Research Division, Pediatric Oncology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA A
Dahlberg, C Delaney & I D Bernstein * Department of Pediatrics, University of Washington, Seattle, Washington, USA A Dahlberg, C Delaney & I D Bernstein * Vaccine and Infectious
Disease Division, PS Statistics, Fred Hutchinson Cancer Research Center, Seattle, WA, USA S Woo & R Gottardo * Broad Institute of MIT and Harvard, Cambridge, MA, USA P Boyle, A Gnirke, C
Bock, B E Bernstein & A Meissner * Howard Hughes Medical Institute, Chevy Chase, MD, USA B E Bernstein * Department of Pathology, Massachusetts General Hospital and Harvard Medical
School, Boston, MA, USA B E Bernstein Authors * A Dahlberg View author publications You can also search for this author inPubMed Google Scholar * S Woo View author publications You can also
search for this author inPubMed Google Scholar * C Delaney View author publications You can also search for this author inPubMed Google Scholar * P Boyle View author publications You can
also search for this author inPubMed Google Scholar * A Gnirke View author publications You can also search for this author inPubMed Google Scholar * C Bock View author publications You can
also search for this author inPubMed Google Scholar * B E Bernstein View author publications You can also search for this author inPubMed Google Scholar * A Meissner View author publications
You can also search for this author inPubMed Google Scholar * R Gottardo View author publications You can also search for this author inPubMed Google Scholar * I D Bernstein View author
publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to I D Bernstein. ETHICS DECLARATIONS COMPETING INTERESTS The Fred Hutchinson
Cancer Research Center holds a patent on ‘methods for immortalizing cells’ that covers the use of Notch ligand for expansion of hematopoietic stem cells. IDB is an inventor on this patent.
The remaining authors declare no conflict of interest. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Dahlberg, A., Woo, S., Delaney, C. _et al._
Notch-mediated expansion of cord blood progenitors: maintenance of transcriptional and epigenetic fidelity. _Leukemia_ 29, 1948–1951 (2015). https://doi.org/10.1038/leu.2015.61 Download
citation * Published: 06 March 2015 * Issue Date: September 2015 * DOI: https://doi.org/10.1038/leu.2015.61 SHARE THIS ARTICLE Anyone you share the following link with will be able to read
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