ABSTRACT Phyllotaxy describes the geometric pattern of leaves and flowers, and has intrigued botanists and mathematicians for centuries1,2. How these patterns are initiated is poorly

understood, and this is partly due to the paucity of mutants3. Signalling by the plant hormone auxin appears to determine the site of leaf initiation; however, this observation does not

explain how distinct patterns of phyllotaxy are initiated4. _abphyl1_ (_abph1_) mutants of maize initiate leaves in a decussate pattern (that is, paired at 180°), in contrast to the

alternating or distichous phyllotaxy observed in wild-type maize and other grasses5. Here we show that _ABPH1_ is homologous to two-component response regulators and is induced by the plant

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SIMILAR CONTENT BEING VIEWED BY OTHERS COORDINATION OF SHOOT APICAL MERISTEM SHAPE AND IDENTITY BY APETALA2 DURING FLORAL TRANSITION IN ARABIDOPSIS Article Open access 13 August 2024

SPECIFICATION OF LEAF DORSIVENTRALITY VIA A PREPATTERNED BINARY READOUT OF A UNIFORM AUXIN INPUT Article 22 March 2022 A NETWORK OF CLAVATA RECEPTORS BUFFERS AUXIN-DEPENDENT MERISTEM

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meristem proliferation in maize. _Genes Dev._ 15, 2755–2766 (2001) Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS We thank V. Chandler for _Spm_ transposon lines, and

members of the Jackson laboratory, C. Kidner, E. Vollbrecht and P. Sherwood, for comments on the manuscript. We also thank Z. Yuan and M. Krishnaswami for assistance with genetic screens,

DNA isolations and Southern blotting, and T. Mulligan for help with plant propagation. Funding from the National Science Foundation (Plant and Animal Developmental Mechanisms) is also

acknowledged. AUTHOR INFORMATION Author notes * Anna Giulini Present address: Department of Plant Production, University of Milan, Via Celoria 2, 20133, Milan, Italy AUTHORS AND AFFILIATIONS

* Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York, 11724, USA Anna Giulini, Jing Wang & David Jackson Authors * Anna Giulini View author publications You

can also search for this author inPubMed Google Scholar * Jing Wang View author publications You can also search for this author inPubMed Google Scholar * David Jackson View author

publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to David Jackson. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare

that they have no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY DATA This file contains Supplementary Figure S1 (Molecular characterization of _abph1_ alleles),

which shows the molecular analysis of a number of _abph1_ alleles. These alleles include ones from transposon screens as well as spontaneous alleles. The demonstration of a molecular lesion

in the candidate locus for each indicates that this locus encodes _ABPH1_. Supplementary Figure S2 (in situ hybridization analysis of _ABPH1_ expression), which shows the expression of ABPH1

by _in situ_ hybridization in a transverse section of a seedling apex. Expression is in an arc of cells in the position of the incipient leaf primordium. Expression throughout the ear

inflorescence apical meristem is also shown. Supplementary references are also provided. (DOC 687 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE

Giulini, A., Wang, J. & Jackson, D. Control of phyllotaxy by the cytokinin-inducible response regulator homologue _ABPHYL1_. _Nature_ 430, 1031–1034 (2004).

https://doi.org/10.1038/nature02778 Download citation * Received: 25 April 2004 * Accepted: 22 June 2004 * Issue Date: 26 August 2004 * DOI: https://doi.org/10.1038/nature02778 SHARE THIS

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