ABSTRACT Replying to: J. T. Bennett _et al._ _Nature_ 450, doi:10.1038/nature06314 (2007) We presented several lines of evidence indicating that dorso–ventral asymmetry is apparent in

zebrafish embryos by cleavage stages1, one of which showed that injection of three different morpholino oligonucleotides targeting three different _squint_ (_sqt_) sequences cause severe

disruption in dorsal structures. We concluded that the dorsal axis is evident by the 4-cell stage, and suggested that maternal Sqt and associated factors may function in zebrafish

dorsal-axis formation1. Bennett _et al._ challenge our results obtained with morpholino oligonucleotides because they do not find a comparable defect in maternal and zygotic _sqt_ mutants2.

Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer

support SIMILAR CONTENT BEING VIEWED BY OTHERS LOSS OF KRÜPPEL-LIKE FACTOR 9 DEREGULATES BOTH PHYSIOLOGICAL GENE EXPRESSION AND DEVELOPMENT Article Open access 28 July 2023 EMBRYO-SCALE

REVERSE GENETICS AT SINGLE-CELL RESOLUTION Article Open access 15 November 2023 _SETD2_ KNOCKOUT ZEBRAFISH IS VIABLE AND FERTILE: DIFFERENTIAL AND DEVELOPMENTAL STRESS-RELATED REQUIREMENTS

FOR SETD2 AND HISTONE H3K36 TRIMETHYLATION IN DIFFERENT VERTEBRATE ANIMALS Article Open access 20 October 2020 REFERENCES * Gore, A. V. et al. The zebrafish dorsal axis is apparent at the

four-cell stage. _Nature_ 438, 1030–1035 (2005) Article  CAS  ADS  Google Scholar  * Bennett, J. T. et al. Maternal nodal and zebrafish embryogenesis. _Nature_ 450, 10.1038/nature06314.

(2007) * Feldman, B. & Stemple, D. L. Morpholino phenocopies of sqt, oep, and ntl mutations. _Genesis_ 30, 175–177 (2001) Article  CAS  Google Scholar  * Feldman, B. et al. Zebrafish

organizer development and germ-layer formation require nodal-related signals. _Nature_ 395, 181–185 (1998) Article  CAS  ADS  Google Scholar  * Aoki, T. O. et al. Regulation of nodal

signalling and mesendoderm formation by TARAM-A, a TGFβ-related type I receptor. _Dev. Biol._ 241, 273–288 (2002) Article  CAS  Google Scholar  * Simon, R., Wu, L. & Richter, J. D.

Cytoplasmic polyadenylation of activin receptor mRNA and the control of pattern formation in _Xenopus_ development. _Dev. Biol._ 179, 239–250 (1996) Article  CAS  Google Scholar  * Pandur,

P. D., Sullivan, S. A. & Moody, S. A. Multiple maternal influences on dorsal-ventral fate of _Xenopus_ animal blastomeres. _Dev. Dyn._ 225, 581–587 (2002) Article  CAS  Google Scholar  *

Vasudevan, S., Seli, E. & Steitz, J. A. Metazoan oocyte and early embryo development program: a progression through translation regulatory cascades. _Genes Dev._ 20, 138–146 (2006)

Article  CAS  Google Scholar  * Rebagliati, M. R., Toyama, R., Fricke, C., Haffter, P. & Dawid, I. B. Zebrafish nodal-related genes are implicated in axial patterning and establishing

left-right asymmetry. _Dev. Biol._ 199, 261–272 (1998) Article  CAS  Google Scholar  * Dougan, S. T., Warga, R. M., Kane, D. A., Schier, A. F. & Talbot, W. S. The role of the zebrafish

nodal-related genes squint and cyclops in patterning of mesendoderm. _Development_ 130, 1837–1851 (2003) Article  CAS  Google Scholar  * Erter, C. E., Solnica-Krezel, L. & Wright, C. V.

Zebrafish nodal-related 2 encodes an early mesendodermal inducer signaling from the extraembryonic yolk syncytial layer. _Dev. Biol._ 204, 361–372 (1998) Article  CAS  Google Scholar  *

Gore, A. V. _Localized Molecules and the Establishment of Polarity in Zebrafish_. PhD thesis, National Univ. Singapore. (2006) Google Scholar  * Pei, W., Williams, P. H., Clark, M. D.,

Stemple, D. L. & Feldman, B. Environmental and genetic modifiers of squint penetrance during zebrafish embryogenesis. _Dev. Biol._ 309, 245–258 (2007) Article  Google Scholar  * Hagos,

E. G., Fan, X. & Dougan, S. T. The role of maternal Activin-like signals in zebrafish embryos. _Dev. Biol._ 308 368–378 doi: 10.1016/j.ydbio.2007.07.010 (2007) Article  CAS  Google

Scholar  * Kloc, M. et al. Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of _Xenopus_ oocytes. _Development_ 132,

3445–3457 (2005) Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * Aniket V. Gore & Albert Cheong Present address: †Present addresses: Unit on

Vertebrate Organogenesis, Laboratory of Molecular Genetics, The National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA

(A.V.G.); Cell Culture Process Development, Lonza Biologics, 228 Bath Road, Slough, Berkshire, SL1 4DX, UK (A.C.), AUTHORS AND AFFILIATIONS * Vertebrate Development Group, Temasek Life

Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore Aniket V. Gore, Albert Cheong, Patrick C. Gilligan & Karuna Sampath * † School of Biological

Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551 Singapore , Karuna Sampath * Department of Biological Sciences, National University of Singapore, 117543 Singapore Karuna

Sampath Authors * Aniket V. Gore View author publications You can also search for this author inPubMed Google Scholar * Albert Cheong View author publications You can also search for this

author inPubMed Google Scholar * Patrick C. Gilligan View author publications You can also search for this author inPubMed Google Scholar * Karuna Sampath View author publications You can

also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Gore, A., Cheong, A., Gilligan, P. _et al._ _Gore_ et

al. _reply_. _Nature_ 450, E2–E4 (2007). https://doi.org/10.1038/nature06315 Download citation * Issue Date: 08 November 2007 * DOI: https://doi.org/10.1038/nature06315 SHARE THIS ARTICLE

Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided

by the Springer Nature SharedIt content-sharing initiative