ABSTRACT Plants must coordinate the regulation of biochemistry and anatomy to optimize photosynthesis and water-use efficiency. The formation of stomata, epidermal pores that facilitate gas

exchange, is highly coordinated with other aspects of photosynthetic development. The signalling pathways controlling stomata development are not fully understood1,2, although

mitogen-activated protein kinase (MAPK) signalling is known to have key roles. Here we demonstrate in _Arabidopsis_ that brassinosteroid regulates stomatal development by activating the MAPK

kinase kinase (MAPKKK) YDA (also known as YODA). Genetic analyses indicate that receptor kinase-mediated brassinosteroid signalling inhibits stomatal development through the glycogen

with either brassinosteroid or GSK3-kinase inhibitor. Our results indicate that brassinosteroid inhibits stomatal development by alleviating GSK3-mediated inhibition of this MAPK module,

providing two key links; that of a plant MAPKKK to its upstream regulators and of brassinosteroid to a specific developmental output. Access through your institution Buy or subscribe This is

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TRANSCRIPTION FACTOR REGULATES STOMATAL OPENING BY MODULATING OXYLIPIN SYNTHESIS IN GUARD CELLS Article Open access 11 January 2022 HYDROGEN PEROXIDE IS REQUIRED FOR LIGHT-INDUCED STOMATAL

OPENING ACROSS DIFFERENT PLANT SPECIES Article Open access 14 June 2024 DICHOTOMY OF THE BSL PHOSPHATASE SIGNALING SPATIALLY REGULATES MAPK COMPONENTS IN STOMATAL FATE DETERMINATION Article

Open access 04 May 2022 CHANGE HISTORY * _ 08 FEBRUARY 2012 The original supplementary information file posted online was incorrect and has been replaced. _ REFERENCES * Bergmann, D. C.

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proteomics. _Plant J._ 45, 616–629 (2006) Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS We thank K. Torii for providing seeds of the _er erl1 erl2_ triple mutant and

_scrm-D_ mutant, J.-M. Zhou for providing the _HOPAI1_ transgenic line and J. Li for providing the _bin2-3 bil1 bil2_ triple mutant. Research was primarily supported by a grant from the

National Institutes of Health (R01GM066258), and partially supported by the US Department of Energy (DE-FG02-08ER15973) and the Herman Frasch Foundation. D.C.B. is an investigator of the

Howard Hughes Medical Institute. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Plant Biology, Carnegie Institution for Science, Stanford, 94305-4150, California, USA Tae-Wuk

Kim & Zhi-Yong Wang * Department of Life Science, Hanyang University, Seoul 133-791, South Korea, Tae-Wuk Kim * Department of Biology, Stanford University, Stanford, 94305-5020,

California, USA Marta Michniewicz & Dominique C. Bergmann Authors * Tae-Wuk Kim View author publications You can also search for this author inPubMed Google Scholar * Marta Michniewicz

View author publications You can also search for this author inPubMed Google Scholar * Dominique C. Bergmann View author publications You can also search for this author inPubMed Google

Scholar * Zhi-Yong Wang View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS T.-W.K. performed all experiments. T.-W.K. and Z.-Y.W. designed the

experiments, analysed data and wrote the manuscript. M.M. cloned complementary DNAs of BSL2 and BSL3, and BSL2pro-BSL2. D.C.B. contributed materials and wrote the manuscript. CORRESPONDING

AUTHOR Correspondence to Zhi-Yong Wang. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION This

file contains Supplementary Figures 1-13 with legends. _The original file posted online was incorrect and was replaced on 8 February 2012._ (PDF 4371 kb) POWERPOINT SLIDES POWERPOINT SLIDE

FOR FIG. 1 POWERPOINT SLIDE FOR FIG. 2 POWERPOINT SLIDE FOR FIG. 3 RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kim, TW., Michniewicz, M., Bergmann,

D. _et al._ Brassinosteroid regulates stomatal development by GSK3-mediated inhibition of a MAPK pathway. _Nature_ 482, 419–422 (2012). https://doi.org/10.1038/nature10794 Download citation

* Received: 18 March 2011 * Accepted: 13 December 2011 * Published: 05 February 2012 * Issue Date: 16 February 2012 * DOI: https://doi.org/10.1038/nature10794 SHARE THIS ARTICLE Anyone you

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