ABSTRACT The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating

specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a given human leukocyte antigen (HLA) type. Mouse

fibroblasts were retrovirally transduced with a single HLA–peptide complex along with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit strong

stimulation and expansion of HLA-restricted CTLs. Owing to the high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily engineered for any HLA molecule and any

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Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS NANOPARTICLE-BASED MODULATION OF CD4+ T CELL EFFECTOR AND HELPER FUNCTIONS ENHANCES ADOPTIVE IMMUNOTHERAPY Article Open access

14 October 2022 EXPANSION OF MIXED IMMUNE CELLS USING CD3/CD161 CO-STIMULATION FOR THE TREATMENT OF CANCER Article Open access 26 April 2023 A SINGLE-CHAIN ANTIBODY GENERATION SYSTEM

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Download references ACKNOWLEDGEMENTS We thank C. Tan (Gene Transfer and Somatic Cell Engineering Facility, MSKCC) for excellent technical assistance, T. Delohery (Flow Cytometry Core

Facility, MSKCC) for excellent assistance with cell sorting, and Dr. I. Rivière for reviewing the manuscript. This work was supported by grant CA-59350, the Dewitt Wallace Fund, and the

McDonnell Scholars Award for Molecular Medicine (M.S.). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Human Genetics Immunology Program, Memorial Sloan-Kettering Cancer Center,

New York, 10021, NY Jean-Baptiste Latouche & Michel Sadelain Authors * Jean-Baptiste Latouche View author publications You can also search for this author inPubMed Google Scholar *

Michel Sadelain View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Michel Sadelain. RIGHTS AND PERMISSIONS Reprints

and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Latouche, JB., Sadelain, M. Induction of human cytotoxic T lymphocytes by artificial antigen-presenting cells. _Nat Biotechnol_ 18,

405–409 (2000). https://doi.org/10.1038/74455 Download citation * Received: 10 January 2000 * Accepted: 02 February 2000 * Issue Date: April 2000 * DOI: https://doi.org/10.1038/74455 SHARE

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