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Access through your institution Buy or subscribe The proteome has been defined as the entire complement of proteins expressed by a cell, organism, or tissue type, and accordingly, proteomics
is the study of this complement expressed at a given time or under certain environmental conditions. However, such a global perspective requires that thousands of proteins be routinely
identified and characterized from a single sample; only then can we infer how protein function relates to cellular pathways, modules, and networks, and how new (emergent) properties arise
for the larger “system”. These measurements must be made at the protein level because, among other reasons, protein abundance cannot be predicted from mRNA abundance1, and post-translational
modifications cannot be directly predicted from deduced amino acid sequence. Simply predicting a polypeptide sequence can even be problematic because of alternative splicing of mRNAs or
frameshifts2. The number of spots observed in 2D-PAGE displays is often comparable to the number of proteins predicted for a given organism or tissue sample, hinting that a substantially
complete view of proteomes is within reach. However, the technical difficulties inherent in 2D-PAGE, such as limited solubility of hydrophobic and membrane proteins, limited dynamic range,
and difficulties in focusing highly basic and acidic proteins, make an accurate assessment of proteome coverage elusive. Moreover, methods for identifying the protein spots have been
insensitive, slow, expensive, and often ineffective. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this
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leader and chief scientist at the Pacific Northwest National Laboratory, Richland, 99352, WA Richard D. Smith Authors * Richard D. Smith View author publications You can also search for this
author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Smith, R. Probing proteomes—seeing the whole picture?. _Nat Biotechnol_
18, 1041–1042 (2000). https://doi.org/10.1038/80226 Download citation * Issue Date: October 2000 * DOI: https://doi.org/10.1038/80226 SHARE THIS ARTICLE Anyone you share the following link
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