ABSTRACT A novel microfiltration batch culture process, suitable for scale-up, has been designed, instrumented, and studied with a representative recombinant-DNA receptor strain of

_Escherichia coli_. In this process nutrient medium is fed continuously into an aerated and stirred fermentor, and metabolic products are withdrawn continuously at the same rate by crossflow

microfiltration in an external recycle loop, so that product inhibition of cell growth is relieved. The maximum density of cells is increased fivefold more than in the control process, and

is limited by acetic and other organic acids accumulating from the aerobic fermentation of excess glucose. Access through your institution Buy or subscribe This is a preview of subscription

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for GC analysis of culture by-products for volatile acids and alcohols. Bulletin Supplement 748G. Download references AUTHOR INFORMATION Author notes * Philipp Gerhardt: To whom

correspondence should be directed. AUTHORS AND AFFILIATIONS * Department of Chemical Engineering, Michigan State University, East Lansing, MI, 48824 Kevin W. Anderson & Eric Grulke *

Department of Microbiology and Public Health, Michigan State University, East Lansing, MI, 48824 Philipp Gerhardt Authors * Kevin W. Anderson View author publications You can also search for

this author inPubMed Google Scholar * Eric Grulke View author publications You can also search for this author inPubMed Google Scholar * Philipp Gerhardt View author publications You can

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Microfiltration Culture Process for Enhanced Production of rDNA Receptor Cells of _Escherichia Coli_. _Nat Biotechnol_ 2, 891–896 (1984). https://doi.org/10.1038/nbt1084-891 Download

citation * Received: 19 April 1984 * Accepted: 12 June 1984 * Issue Date: 01 October 1984 * DOI: https://doi.org/10.1038/nbt1084-891 SHARE THIS ARTICLE Anyone you share the following link

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