Automation of dna sequencing reactions and related techniques: a workstation for micromanipulation of liquids

Automation of dna sequencing reactions and related techniques: a workstation for micromanipulation of liquids

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ABSTRACT We describe the construction of a laboratory workstation for the micromanipulation of liquids. The performance of the workstation is demonstrated by its use in a DNA sequencing protocol; although its operational units form the backbone of a robotic system with wide applications in molecular biology and biochemistry. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS PRINCIPLES OF COMPUTER-CONTROLLED LINEAR MOTION APPLIED TO AN OPEN-SOURCE AFFORDABLE LIQUID HANDLER FOR AUTOMATED MICROPIPETTING Article Open access 12 August 2020 SOFT ROBOTIC PATTERNING OF LIQUIDS Article Open access 21 September 2023 AUTOMATING LIFE SCIENCE LABS AT THE SINGLE-CELL LEVEL THROUGH PRECISE ULTRASONIC LIQUID SAMPLE EJECTION: PULSE Article Open access 20 November 2024 REFERENCES * Sanger, F., Niklen, S., and Coulson, A.R. 1977. DNA sequencing with chain terminating inhibitors. _Proc. Natl. Acad. Sci. USA_ 74:5463–5467. Article  CAS  Google Scholar  * Saiki, R.K., Bugawan, T.L., Horn, G.T., Mullis, K.B., and Erlich, H.A. 1986. Analysis of enzymatically amplified b-globin and HLA-DQ_a_ DNA with allele-specific oligonucleotide probes. _Nature_ 324:163–166. Article  CAS  Google Scholar  * Dale, R.M.K., McClure, B.A., and Houchins, J.P. 1985. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: Application to sequencing the corn mitochondrial 18S rDNA. _Plasmid_ 13:31–40. Article  CAS  Google Scholar  * Martin, W.J., Warmington, J.R., Galinski, B.R., Gallagher, M., Davies, R.W., Beck, M.S., and Oliver, S.G. 1985. Automation of DNA sequencing: A system to perform the sanger dideoxysequencing reactions. _Bio/Technology_ 3:911–915. Article  CAS  Google Scholar  * Martin, W.J., Galinski, B.R., and Beck, M.S. 1987. Automatic manipulation of microlitre volumes of liquid reagents. _J. Phys. E: Sci. Instrum._ 20:22–26. Article  CAS  Google Scholar  * Tabor, S. and Richardson, C.C. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. _Proc. Natl. Acad. Sci. USA_ 84:4767–4771. Article  CAS  Google Scholar  * Smith, L.M., Sanders, U.Z., Kaiser, R.J., Hughes, P., Dodd, C., Connell, C.R., Heiner, C., Kent, S.B.H., and Hood, L.E. 1986. Fluorescence detection in automated DNA sequence analysis. _Nature_ 321:674–679. Article  CAS  Google Scholar  * Ansorge, W., Sproat, B.S., Stegemann, J., and Schwager, C. 1986. A nonradioactive automated method for DNA sequence determination. _J. Biochem. Biophys. Meth._ 13:315–323. Article  CAS  Google Scholar  * Stoflet, E.S., Koeberl, D.D., Sarkar, G., and Sommer, S.S. 1988. Genomic amplification with transcript sequencing. _Science_ 239:491–494. Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * Rainer Frank: Author to whom correspondence should be sent. AUTHORS AND AFFILIATIONS * European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg, F.R. Germany Rainer Frank, Armin Bosserhoff, Christian Boulin, Avi Epstein, Heinrich Gausepohl & Keith Ashman Authors * Rainer Frank View author publications You can also search for this author inPubMed Google Scholar * Armin Bosserhoff View author publications You can also search for this author inPubMed Google Scholar * Christian Boulin View author publications You can also search for this author inPubMed Google Scholar * Avi Epstein View author publications You can also search for this author inPubMed Google Scholar * Heinrich Gausepohl View author publications You can also search for this author inPubMed Google Scholar * Keith Ashman View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Frank, R., Bosserhoff, A., Boulin, C. _et al._ Automation of DNA Sequencing Reactions and Related Techniques: A Workstation for Micromanipulation of Liquids. _Nat Biotechnol_ 6, 1211–1213 (1988). https://doi.org/10.1038/nbt1088-1211 Download citation * Received: 18 April 1988 * Accepted: 21 June 1988 * Issue Date: 01 October 1988 * DOI: https://doi.org/10.1038/nbt1088-1211 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

ABSTRACT We describe the construction of a laboratory workstation for the micromanipulation of liquids. The performance of the workstation is demonstrated by its use in a DNA sequencing


protocol; although its operational units form the backbone of a robotic system with wide applications in molecular biology and biochemistry. Access through your institution Buy or subscribe


This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access


$209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are


calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS


PRINCIPLES OF COMPUTER-CONTROLLED LINEAR MOTION APPLIED TO AN OPEN-SOURCE AFFORDABLE LIQUID HANDLER FOR AUTOMATED MICROPIPETTING Article Open access 12 August 2020 SOFT ROBOTIC PATTERNING OF


LIQUIDS Article Open access 21 September 2023 AUTOMATING LIFE SCIENCE LABS AT THE SINGLE-CELL LEVEL THROUGH PRECISE ULTRASONIC LIQUID SAMPLE EJECTION: PULSE Article Open access 20 November


2024 REFERENCES * Sanger, F., Niklen, S., and Coulson, A.R. 1977. DNA sequencing with chain terminating inhibitors. _Proc. Natl. Acad. Sci. USA_ 74:5463–5467. Article  CAS  Google Scholar  *


Saiki, R.K., Bugawan, T.L., Horn, G.T., Mullis, K.B., and Erlich, H.A. 1986. Analysis of enzymatically amplified b-globin and HLA-DQ_a_ DNA with allele-specific oligonucleotide probes.


_Nature_ 324:163–166. Article  CAS  Google Scholar  * Dale, R.M.K., McClure, B.A., and Houchins, J.P. 1985. A rapid single-stranded cloning strategy for producing a sequential series of


overlapping clones for use in DNA sequencing: Application to sequencing the corn mitochondrial 18S rDNA. _Plasmid_ 13:31–40. Article  CAS  Google Scholar  * Martin, W.J., Warmington, J.R.,


Galinski, B.R., Gallagher, M., Davies, R.W., Beck, M.S., and Oliver, S.G. 1985. Automation of DNA sequencing: A system to perform the sanger dideoxysequencing reactions. _Bio/Technology_


3:911–915. Article  CAS  Google Scholar  * Martin, W.J., Galinski, B.R., and Beck, M.S. 1987. Automatic manipulation of microlitre volumes of liquid reagents. _J. Phys. E: Sci. Instrum._


20:22–26. Article  CAS  Google Scholar  * Tabor, S. and Richardson, C.C. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. _Proc. Natl. Acad. Sci. USA_


84:4767–4771. Article  CAS  Google Scholar  * Smith, L.M., Sanders, U.Z., Kaiser, R.J., Hughes, P., Dodd, C., Connell, C.R., Heiner, C., Kent, S.B.H., and Hood, L.E. 1986. Fluorescence


detection in automated DNA sequence analysis. _Nature_ 321:674–679. Article  CAS  Google Scholar  * Ansorge, W., Sproat, B.S., Stegemann, J., and Schwager, C. 1986. A nonradioactive


automated method for DNA sequence determination. _J. Biochem. Biophys. Meth._ 13:315–323. Article  CAS  Google Scholar  * Stoflet, E.S., Koeberl, D.D., Sarkar, G., and Sommer, S.S. 1988.


Genomic amplification with transcript sequencing. _Science_ 239:491–494. Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * Rainer Frank: Author to whom


correspondence should be sent. AUTHORS AND AFFILIATIONS * European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg, F.R. Germany Rainer Frank, Armin Bosserhoff, Christian


Boulin, Avi Epstein, Heinrich Gausepohl & Keith Ashman Authors * Rainer Frank View author publications You can also search for this author inPubMed Google Scholar * Armin Bosserhoff View


author publications You can also search for this author inPubMed Google Scholar * Christian Boulin View author publications You can also search for this author inPubMed Google Scholar * Avi


Epstein View author publications You can also search for this author inPubMed Google Scholar * Heinrich Gausepohl View author publications You can also search for this author inPubMed 


Google Scholar * Keith Ashman View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE


THIS ARTICLE Frank, R., Bosserhoff, A., Boulin, C. _et al._ Automation of DNA Sequencing Reactions and Related Techniques: A Workstation for Micromanipulation of Liquids. _Nat Biotechnol_ 6,


1211–1213 (1988). https://doi.org/10.1038/nbt1088-1211 Download citation * Received: 18 April 1988 * Accepted: 21 June 1988 * Issue Date: 01 October 1988 * DOI:


https://doi.org/10.1038/nbt1088-1211 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not


currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative