ABSTRACT Ras proteins control the signalling pathways that are responsible for normal growth and malignant transformation1. Raf protein kinases are direct Ras effector proteins that initiate

the mitogen-activated protein kinase (MAPK) cascade2, which mediates diverse biological functions such as cell growth, survival and differentiation3. Here we show that prohibitin, a

ubiquitously expressed and evolutionarily conserved protein4 is indispensable for the activation of the Raf–MEK–ERK pathway by Ras. The membrane targeting and activation of C-Raf by Ras

needs prohibitin in vivo. In addition, direct interaction with prohibitin is required for C-Raf activation. C-Raf kinase fails to interact with the active Ras induced by epidermal growth

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SIMILAR CONTENT BEING VIEWED BY OTHERS RAS SUPPRESSION POTENTIATES REAR ACTOMYOSIN CONTRACTILITY-DRIVEN CELL POLARIZATION AND MIGRATION Article 01 July 2024 AFADIN COUPLES RAS GTPASES TO THE

POLARITY RHEOSTAT SCRIBBLE Article Open access 05 August 2022 ACTIVE R-RAS2/TC21 PREVENTS CELL CYCLE ARREST AND MORPHOLOGICAL ALTERATIONS IN MOUSE EMBRYONIC FIBROBLASTS LACKING RAS PROTEINS

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1185–1197 (1994). Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS We thank M. Oswald, D. Khalil, C. Dimmler, B. Fauler and U. Reichard for excellent technical assistance,

T. Fowler for critical reading of the manuscript and the EURIT team for their help with siRNA validation. M. Selbach is thanked for kindly providing the Ras constructs and S. Lohmann for the

VASP-P157 antibodies. This work was supported by grants from the Bundesministerium für Bildung und Forschung (BMBF) to T.R. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of

Molecular Biology, Max Planck Institute for Infection Biology, Schumannstr. 21/22, Berlin, D-10117, Germany Krishnaraj Rajalingam, Christian Wunder, Yuri Churin, Claudia Sievers & Thomas

Rudel * Microscopy Core Facility, Schumannstr. 21/22, Berlin, D-10117, Germany Volker Brinkmann * Institut für Medizinische Strahlenkunde und Zellforschung, University of Würzburg,

Versbacher Strasse 5, Würzburg, 97078, Germany Mirko Hekman & Ulf R. Rapp Authors * Krishnaraj Rajalingam View author publications You can also search for this author inPubMed Google

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Thomas Rudel. ETHICS DECLARATIONS COMPETING INTERESTS A patent application has been filed. SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Supplementary figures S1, S2, S3 and S4;

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_et al._ Prohibitin is required for Ras-induced Raf–MEK–ERK activation and epithelial cell migration. _Nat Cell Biol_ 7, 837–843 (2005). https://doi.org/10.1038/ncb1283 Download citation *

Received: 11 May 2005 * Accepted: 28 June 2005 * Published: 24 July 2005 * Issue Date: August 2005 * DOI: https://doi.org/10.1038/ncb1283 SHARE THIS ARTICLE Anyone you share the following

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