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Access through your institution Buy or subscribe To the Editor: Recently, Frey and colleagues predicted more than 1.14 million putative exon sequences from the mouse genome, among which are
155,839 sequences identified using exon microarrays and the GenRate algorithm1. In order to directly compare these putative exons with protein sequences, we selected 74,377 peptides (termed
'mass-identified peptides' and corresponding to 18,744 proteins) from our mouse liver proteomic data set (S.J. Li _et al_., unpublished data) using highly stringent criteria
(Supplementary Methods online). To simplify the comparison, we selected all three positive-strand frames of 1,123,691 exons provided by Frey _et al_.1; then, from these positive-strand
frames, we theoretically translated 6,949,321 putative peptides consisting of at least six amino acids (termed 'exon-translated peptides'). This is a preview of subscription
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ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Frey, B.J. et al. _Nat. Genet._ 37, 991–996 (2005). Article CAS
Google Scholar * Bertone, P. et al. _Science_ 306, 2242–2246 (2004). Article CAS Google Scholar * Gygi, S.P. et al. _Mol. Cell. Biol._ 19, 1720–1730 (1999). Article CAS Google Scholar
* Futcher, B. et al. _Mol. Cell. Biol._ 19, 7357–7368 (1999). Article CAS Google Scholar Download references ACKNOWLEDGEMENTS We are grateful to D. Li for critical revision of the
manuscript. This work was supported by grants from the Chinese National Natural Science Foundation to J.-R.W. and R.Z. (30230110, 0637S12442 and 30425021). AUTHOR INFORMATION AUTHORS AND
AFFILIATIONS * State Key Laboratory of Molecular Biology, Chinese Academy of Sciences, 320 YueYang Road, Shanghai, 200031, China Quan-Hu Sheng, Rong Zeng & Jia-Rui Wu * Research Center
for Proteome Analysis, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 YueYang Road, Shanghai, 200031, China Quan-Hu Sheng, Lian-Shui Wang, Jie Dai, Xiao-Sheng
Jiang, Rong Xia Li, Dan-Jun Ma & Rong Zeng * Bioinformation Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai, 200031, China
Quan-Hu Sheng & Yi-Xue Li * Hefei National Laboratory for Physical Sciences at a Microscale and School of Life Science, University of Science and Technology of China, Hefei, Anhui, China
Jia-Rui Wu * Jia-Rui Wu Authors * Quan-Hu Sheng View author publications You can also search for this author inPubMed Google Scholar * Lian-Shui Wang View author publications You can also
search for this author inPubMed Google Scholar * Jie Dai View author publications You can also search for this author inPubMed Google Scholar * Xiao-Sheng Jiang View author publications You
can also search for this author inPubMed Google Scholar * Rong Xia Li View author publications You can also search for this author inPubMed Google Scholar * Dan-Jun Ma View author
publications You can also search for this author inPubMed Google Scholar * Yi-Xue Li View author publications You can also search for this author inPubMed Google Scholar * Rong Zeng View
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SUPPLEMENTARY INFORMATION SUPPLEMENTARY TABLE 1 Matches of mass-identified peptides and exons that passed primary filters. (PDF 1351 kb) SUPPLEMENTARY TABLE 2 Matches of mass-identified
peptides and exons that passed stringent filters. (PDF 963 kb) SUPPLEMENTARY TABLE 3 RefSeq genes corresponding to the group of 28 exons with the highest abundance and matched peptides. (PDF
20 kb) SUPPLEMENTARY METHODS (PDF 70 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sheng, QH., Wang, LS., Dai, J. _et al._ Comparison of a
proteomic approach with a microarray-based approach to detect exons in the mouse genome. _Nat Genet_ 38, 1223–1224 (2006). https://doi.org/10.1038/ng1106-1223 Download citation * Issue Date:
01 November 2006 * DOI: https://doi.org/10.1038/ng1106-1223 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a
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