Expression profiling of soft tissue sarcomas

Expression profiling of soft tissue sarcomas

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Access through your institution Buy or subscribe Soft tissue sarcomas involve the connective tissues of the limbs, girdle, trunk, head and neck. Despite the numerous subtypes of sarcomas (synovial sarcoma, fibrosarcoma and malignant fibrous histiocytoma), tumours in a particular subtype behave in a reasonably consistent fashion, allowing predictions of recurrence and metastasis. Histological and cytogenetic techniques can group most sarcomas; however, the diagnosis can be problematic and must be performed by experts. We are using DNA microarrays to analyse the expression pattern of genes in soft tissue to identify expression signatures specific to the various sarcoma subtypes. Our DNA expression arrays are based on cDNAs from the UniGene set of ESTs. PCR products from an initial set of 3740 cDNAs were gridded onto poly-L-lysine-coated glass microscope slides at a density of 378 spots/cm2. Hybridisations were performed using Cy3- and Cy5-labelled first strand cDNA from cell lines or sarcomas. After washing, the arrays were scanned using a General Scanning Scanarray3000 scanner. The results of this work will be discussed. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Molecular Carcinogenesis, Institute of Cancer Research, Sutton, SM2 5NG, UK Karine Maillard, Karen Barker, Keith Aubin, Melanie Edema, Cyril Fisher & Richard Wooster * Department of Histopathology, Royal Marsden NHS Trust, London, SW3 6JJ, UK Karine Maillard, Karen Barker, Keith Aubin, Melanie Edema, Cyril Fisher & Richard Wooster Authors * Karine Maillard View author publications You can also search for this author inPubMed Google Scholar * Karen Barker View author publications You can also search for this author inPubMed Google Scholar * Keith Aubin View author publications You can also search for this author inPubMed Google Scholar * Melanie Edema View author publications You can also search for this author inPubMed Google Scholar * Cyril Fisher View author publications You can also search for this author inPubMed Google Scholar * Richard Wooster View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Maillard, K., Barker, K., Aubin, K. _et al._ Expression profiling of soft tissue sarcomas. _Nat Genet_ 23 (Suppl 3), 61 (1999). https://doi.org/10.1038/14361 Download citation * Issue Date: November 1999 * DOI: https://doi.org/10.1038/14361 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

Access through your institution Buy or subscribe Soft tissue sarcomas involve the connective tissues of the limbs, girdle, trunk, head and neck. Despite the numerous subtypes of sarcomas


(synovial sarcoma, fibrosarcoma and malignant fibrous histiocytoma), tumours in a particular subtype behave in a reasonably consistent fashion, allowing predictions of recurrence and


metastasis. Histological and cytogenetic techniques can group most sarcomas; however, the diagnosis can be problematic and must be performed by experts. We are using DNA microarrays to


analyse the expression pattern of genes in soft tissue to identify expression signatures specific to the various sarcoma subtypes. Our DNA expression arrays are based on cDNAs from the


UniGene set of ESTs. PCR products from an initial set of 3740 cDNAs were gridded onto poly-L-lysine-coated glass microscope slides at a density of 378 spots/cm2. Hybridisations were


performed using Cy3- and Cy5-labelled first strand cDNA from cell lines or sarcomas. After washing, the arrays were scanned using a General Scanning Scanarray3000 scanner. The results of


this work will be discussed. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print


issues and online access $209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to


local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support AUTHOR INFORMATION


AUTHORS AND AFFILIATIONS * Molecular Carcinogenesis, Institute of Cancer Research, Sutton, SM2 5NG, UK Karine Maillard, Karen Barker, Keith Aubin, Melanie Edema, Cyril Fisher & Richard


Wooster * Department of Histopathology, Royal Marsden NHS Trust, London, SW3 6JJ, UK Karine Maillard, Karen Barker, Keith Aubin, Melanie Edema, Cyril Fisher & Richard Wooster Authors *


Karine Maillard View author publications You can also search for this author inPubMed Google Scholar * Karen Barker View author publications You can also search for this author inPubMed 


Google Scholar * Keith Aubin View author publications You can also search for this author inPubMed Google Scholar * Melanie Edema View author publications You can also search for this author


inPubMed Google Scholar * Cyril Fisher View author publications You can also search for this author inPubMed Google Scholar * Richard Wooster View author publications You can also search


for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Maillard, K., Barker, K., Aubin, K. _et al._ Expression profiling


of soft tissue sarcomas. _Nat Genet_ 23 (Suppl 3), 61 (1999). https://doi.org/10.1038/14361 Download citation * Issue Date: November 1999 * DOI: https://doi.org/10.1038/14361 SHARE THIS


ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard


Provided by the Springer Nature SharedIt content-sharing initiative