ABSTRACT We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein

synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also

overcomes the problem of long-term functional storage of surface-bound proteins. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $259.00 per year only $21.58 per issue Learn more Buy this

TWO-DIMENSIONAL COMPARTMENT GEOMETRY Article 20 July 2020 AN OPEN-SOURCE, 3D PRINTED INKJET DNA SYNTHESIZER Article Open access 14 February 2024 BLUEPRINTING EXTENDABLE NANOMATERIALS WITH

STANDARDIZED PROTEIN BLOCKS Article Open access 13 March 2024 REFERENCES * Bertone, P. & Snyder, M. _FEBS J._ 272, 5400–5411 (2005). Article  CAS  Google Scholar  * Wingren, C. &

Borrebaeck, C.A. _Expert Rev. Proteomics_ 1, 355–364 (2004). Article  CAS  Google Scholar  * Horn, S. et al. _Proteomics_ 6, 605–613 (2006). Article  CAS  Google Scholar  * Wulfkuhle, J.D.,

Edmiston, K.H., Liotta, L.A. & Petricoin, E.F. III. _Nat. Clin. Pract. Oncol._ 3, 256–268 (2006). Article  CAS  Google Scholar  * Stoll, D., Templin, M.F., Bachmann, J. & Joos, T.O.

_Curr. Opin. Drug Discov. Dev._ 8, 239–252 (2005). CAS  Google Scholar  * He, M. & Taussig, M.J. _Nucleic Acids Res._ 29, e73 (2001). Article  CAS  Google Scholar  * Angenendt, P.,

Kreutzberger, J., Glokler, J. & Hoheisel, J.D. _Mol. Cell. Proteomics_ 5, 1658–1666 (2006). Article  CAS  Google Scholar  * Nord, O., Uhlen, M. & Nygren, P.A. _J. Biotechnol._ 106,

1–13 (2003). Article  CAS  Google Scholar  * Ramachandran, N. et al. _Science_ 305, 86–90 (2004). Article  CAS  Google Scholar  * Tao, S.C. & Zhu, H. _Nat. Biotechnol._ 24, 1253–1254

(2006). Article  CAS  Google Scholar  * He, M., Stoevesandt, O. & Taussig, M.J. _Curr. Opin. Biotechnol._ (in the press). * Khan, F., He, M. & Taussig, M.J. _Anal. Chem._ 78,

3072–3079 (2006). Article  CAS  Google Scholar  * MacBeath, G. & Schreiber, S.L. _Science_ 289, 1760–1763 (2000). CAS  PubMed  Google Scholar  * Langlais, C. et al. _BMC Biotechnol._ 7,

64 (2007). Article  Google Scholar  * Murakami, H., Ohta, A., Ashigai, H. & Suga, H. _Nat. Methods_ 3, 357–359 (2006). Article  CAS  Google Scholar  * Devirgiliis, C., Gaetani, S.,

Apreda, M. & Bellovino, D. _Biochem. Biophys. Res. Commun._ 332, 504–511 (2005). Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS We thank H. Liu for expert technical

assistance, B. Korn (DKFZ, Heidelberg) and J. Taipele (University of Helsinki) for providing clones. Research at the Babraham Institute is supported by the Biotechnology and Biological

Sciences Research Council. Work on this project has been supported through the European Commission 6th Framework Programme Integrated Project MolTools. AUTHOR INFORMATION Author notes * Oda

Stoevesandt, Elizabeth A Palmer, Farid Khan & Michael J Taussig Present address: Present addresses: Protein Technology Group, Babraham Bioscience Technologies, Babraham Research Campus,

Cambridge CB22 3AT, UK (O.S. and M.J.T.), MRC Virology Unit, Glasgow G11 5JR, Scotland, UK (E.A.P), and University of Manchester, Manchester Interdisciplinary Biocentre, Manchester, M1 7DN,

UK (F.K.)., * Mingyue He and Oda Stoevesandt: These authors contributed equally to this work. AUTHORS AND AFFILIATIONS * Technology Research Group, The Babraham Institute, Cambridge, CB22

3AT, UK Mingyue He, Oda Stoevesandt, Elizabeth A Palmer, Farid Khan & Michael J Taussig * Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, SE-75185,

Sweden Olle Ericsson Authors * Mingyue He View author publications You can also search for this author inPubMed Google Scholar * Oda Stoevesandt View author publications You can also search

for this author inPubMed Google Scholar * Elizabeth A Palmer View author publications You can also search for this author inPubMed Google Scholar * Farid Khan View author publications You

can also search for this author inPubMed Google Scholar * Olle Ericsson View author publications You can also search for this author inPubMed Google Scholar * Michael J Taussig View author

publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS M.H. and M.J.T. devised the principle of DAPA; M.H. developed and exemplified the method; O.S.

optimized the method, devised the DAPA apparatus and designed and performed the experiments shown in Figures 1 and 2; E.A.P. performed initial DAPA experiments, contributing examples to the

data shown in Supplementary Figures 3 and 4; F.K. characterized the double-6His tag for protein immobilization and protein detection methods; O.E. spotted the DNA template slides for

experiments shown in Figure 1; M.H., O.S. and M.J.T. prepared the manuscript. CORRESPONDING AUTHOR Correspondence to Michael J Taussig. SUPPLEMENTARY INFORMATION SUPPLEMENTARY TEXT AND

FIGURES Supplementary Figures 1–4, Supplementary Methods (PDF 379 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE He, M., Stoevesandt, O., Palmer, E.

_et al._ Printing protein arrays from DNA arrays. _Nat Methods_ 5, 175–177 (2008). https://doi.org/10.1038/nmeth.1178 Download citation * Received: 22 November 2007 * Accepted: 20 December

2007 * Published: 20 January 2008 * Issue Date: February 2008 * DOI: https://doi.org/10.1038/nmeth.1178 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative