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You have full access to this article via your institution. Download PDF Wang, S. _et al_. _Science_ 353, 598–602 (2016). Chromosome conformation capture methods such as Hi-C have been
crucial for studying the structural organization of chromosomal DNA. However, these ensemble measurements may average out structural details, as they occur on the single-chromosome scale. To
bypass this potential limitation, Wang _et al_. developed an imaging-based approach for mapping the spatial organization of chromosomes. Specifically, they mapped structures known from Hi-C
data as topologically associated domains (TADs) using a combination of a modified version of the Oligopaint labeling strategy and a multiplexed _in situ_ hybridization approach previously
developed in the laboratory of Xiaowei Zhuang at Harvard University. Using this approach, they labeled known TADs on several human chromosomes, as well as active and inactive X chromosomes.
Their results correlated well with many results obtained with Hi-C, including the observation of active and inactive regions within individual TADs, and allowed for complementary
discoveries. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Imaging chromosome organization. _Nat Methods_ 13, 713 (2016).
https://doi.org/10.1038/nmeth.3974 Download citation * Published: 30 August 2016 * Issue Date: September 2016 * DOI: https://doi.org/10.1038/nmeth.3974 SHARE THIS ARTICLE Anyone you share
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