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ABSTRACT Transcriptome sequencing is a term that encompasses experiments including mRNA transcript-expression analysis (full-length mRNA, expressed sequence tags (ESTs) and ditags), novel
gene discovery, gene space identification in novel genomes, assembly of full-length genes, single-nucleotide polymorphism (SNP), insertion-deletion and splice-variant discovery, as well as
analyses of allele-specific expression and chromosomal rearrangement. The combination of long, accurate reads and high throughput makes 454 Sequencing™ analysis on the Genome Sequencer FLX
ideally suited to detailed transcriptome investigation. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution RELEVANT
ARTICLES Open Access articles citing this article. * HIGH-THROUGHPUT SEQUENCING-BASED GENE PROFILING ON MULTI-STAGED FRUIT DEVELOPMENT OF DATE PALM (PHOENIX DACTYLIFERA, L.) * Yuxin Yin * ,
Xiaowei Zhang * … Jun Yu _Plant Molecular Biology_ Open Access 21 February 2012 ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online
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are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Sugarbaker, D.J. et al.
Transcriptome sequencing of malignant pleural mesothelioma tumors. _Proc. Natl. Acad. Sci. USA_ 105, 3521–3526 (2008). Article CAS Google Scholar * Toth, A.L. et al. Wasp gene expression
supports an evolutionary link between maternal behavior and eusociality. _Science_ 318, 441–444 (2007). Article CAS Google Scholar * Ng, P. et al. Multiplex sequencing of paired-end
ditags (MS-PET): a strategy for the ultra-high-throughput analysis of transcriptomes and genomes. _Nucleic Acids Res._ 34, e84 (2006). Article Google Scholar * Ng, P. et al. Paired-end
diTagging for transcriptome and genome analysis. _Curr. Protoc. Mol. Biol._ 21.12.1–21.12.42 (2007). * Beldade, P. et al. A wing expressed sequence tag resource for _Bicyclus anynana_
butterflies, an evo-devo model. _BMC Genomics_ 7, 130 (2006). Article Google Scholar Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * 454 Life Sciences, 20 Commercial
Street, Branford, 06405, Connecticut, USA Thomas Jarvie * Roche Diagnostics, Roche Applied Science, 9115 Hague Road, Indianapolis, 46250, Indiana, USA Timothy Harkins Authors * Thomas Jarvie
View author publications You can also search for this author inPubMed Google Scholar * Timothy Harkins View author publications You can also search for this author inPubMed Google Scholar
CORRESPONDING AUTHOR Correspondence to Thomas Jarvie. ADDITIONAL INFORMATION DISCLAIMER This article was submitted to _Nature Methods_ by a commercial organization and has not been peer
reviewed. _Nature Methods_ takes no responsibility for the accuracy or otherwise of the information provided. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS
ARTICLE Jarvie, T., Harkins, T. Transcriptome sequencing with the Genome Sequencer FLX system. _Nat Methods_ 5, vi–viii (2008). https://doi.org/10.1038/nmeth.f.220 Download citation * Issue
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