ABSTRACT Potato late blight, caused by the destructive Irish famine pathogen _Phytophthora infestans_, is a major threat to global food security1,2. All late blight resistance genes

identified to date belong to the coiled-coil, nucleotide-binding, leucine-rich repeat class of intracellular immune receptors3. However, virulent races of the pathogen quickly evolved to

evade recognition by these cytoplasmic immune receptors4. Here we demonstrate that the receptor-like protein ELR (elicitin response) from the wild potato _Solanum microdontum_ mediates

extracellular recognition of the elicitin domain, a molecular pattern that is conserved in _Phytophthora_ species. ELR associates with the immune co-receptor BAK1/SERK3 and mediates

potentially more durable resistance to this devastating plant pathogen. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

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NLR-MEDIATED PLANT IMMUNITY Article 10 March 2021 THE ARABIDOPSIS PATTERN RECOGNITION RECEPTOR EFR ENHANCES FIRE BLIGHT RESISTANCE IN APPLE Article Open access 01 September 2021 A

_PHYTOPHTHORA_ RECEPTOR-LIKE KINASE REGULATES OOSPORE DEVELOPMENT AND CAN ACTIVATE PATTERN-TRIGGERED PLANT IMMUNITY Article Open access 31 July 2023 REFERENCES * Fisher, M. C. _et al._

Emerging fungal threats to animal, plant and ecosystem health. _Nature_ 484, 186–194 (2012). Article  CAS  Google Scholar  * Fry, W. E. _Phytophthora infestans_: the plant (and _R_ gene)

destroyer. _Mol. Plant Pathol._ 9, 385–402 (2008). Article  Google Scholar  * Vleeshouwers, V. G. A. A. _et al._ Understanding and exploiting late blight resistance in the age of effectors.

_Annu. Rev. Phytopathol._ 49, 507–531 (2011). Article  CAS  Google Scholar  * Raffaele, S. _et al._ Genome evolution following host jumps in the Irish potato famine pathogen lineage.

_Science_ 330, 1540–1543 (2010). Article  CAS  Google Scholar  * Haverkort, A., Struik, P., Visser, R. & Jacobsen, E. Applied biotechnology to combat late blight in potato caused by

_Phytophthora infestans_. _Potato Res._ 52, 249–264 (2009). Article  Google Scholar  * Kawchuk, L. M. _et al._ Tomato Ve disease resistance genes encode cell surface-like receptors. _Proc.

Natl Acad. Sci. USA_ 98, 6511–6515 (2001). Article  CAS  Google Scholar  * Jones, D. A., Thomas, C. M., Hammond-Kosack, K. E., Balint-Kurti, P. J. & Jones, J. D. Isolation of the tomato

_Cf9_ gene for resistance to _Cladosporium fulvum_ by transposon tagging. _Science_ 266, 789–793 (1994). Article  CAS  Google Scholar  * Thomma, B. P., Van Esse, H. P., Crous, P. W. & De

Wit, P. J. _Cladosporium fulvum_ (syn. _Passalora fulva_), a highly specialized plant pathogen as a model for functional studies on plant pathogenic _Mycosphaerellaceae_. _Mol. Plant

Pathol._ 6, 379–393 (2005). Article  CAS  Google Scholar  * Ron, M. & Avni, A. The receptor for the fungal elicitor ethylene-inducing xylanase is a member of a resistance-like gene

family in tomato. _Plant Cell_ 16, 1604–1615 (2004). Article  CAS  Google Scholar  * Song, W. Y. _et al._ A receptor kinase-like protein encoded by the rice disease resistance gene _Xa21_.

_Science_ 270, 1804–1806 (1995). Article  CAS  Google Scholar  * Zipfel, C. & Robatzek, S. Pathogen-associated molecular pattern-triggered immunity: veni, vidi...? _Plant Physiol._ 154,

551–554 (2010). Article  CAS  Google Scholar  * Ponchet, M. _et al._ Are elicitins cryptograms in plant-oomycete communications? _Cell. Mol. Life Sci._ 56, 1020–1047 (1999). Article  CAS 

Google Scholar  * Jiang, R. H. Y., Tyler, B. M., Whisson, S. C., Hardham, A. R. & Govers, F. Ancient origin of elicitin gene clusters in _Phytophthora_ genomes. _Mol. Biol. Evol._ 23,

338–351 (2006). Article  CAS  Google Scholar  * Levesque, C. A. _et al._ Genome sequence of the necrotrophic plant pathogen _Pythium ultimum_ reveals original pathogenicity mechanisms and

effector repertoire. _Genome Biol._ 11, R73 (2010). * Cooke, D. E. _et al._ Genome analyses of an aggressive and invasive lineage of the Irish potato famine pathogen. _PLoS Pathog._ 8,

e1002940 (2012). Article  Google Scholar  * Vleeshouwers, V. G. A. A. _et al._ Agroinfection-based high throughput screening reveals specific recognition of INF elicitins in _Solanum_. _Mol.

Plant Pathol._ 7, 499–510 (2006). Article  Google Scholar  * Torto, T. A. _et al._ EST mining and functional expression assays identify extracellular effector proteins from the plant

pathogen _Phytophthora_. _Genome Res._ 13, 1675–1685 (2003). Article  CAS  Google Scholar  * van Os, H. _et al._ Construction of a 10,000-marker ultradense genetic recombination map of

potato: providing a framework for accelerated gene isolation and a genomewide physical map. _Genetics_ 173, 1075–1087 (2006). Article  CAS  Google Scholar  * Potato Genome Sequencing

Consortium _et al_. Genome sequence and analysis of the tuber crop potato. _Nature_ 475, 189–195 (2011). * Chinchilla, D. _et al._ A flagellin-induced complex of the receptor FLS2 and BAK1

initiates plant defence. _Nature_ 448, 497–500 (2007). Article  CAS  Google Scholar  * Heese, A. _et al._ The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in

plants. _Proc. Natl Acad. Sci. USA_ 104, 12217–12222 (2007). Article  CAS  Google Scholar  * Chaparro-Garcia, A. _et al._ The receptor-like kinase SERK3/BAK1 is required for basal resistance

against the late blight pathogen _Phytophthora infestans_ in _Nicotiana benthamiana_. _PLoS ONE_ 6, e16608 (2011). Article  CAS  Google Scholar  * Bar, M. & Avni, A. EHD2 inhibits

signaling of leucine rich repeat receptor-like proteins. _Plant Signal. Behav._ 4, 682–684 (2009). Article  CAS  Google Scholar  * Haas, B. J. _et al._ Genome sequence and analysis of the

Irish potato famine pathogen _Phytophthora infestans_. _Nature_ 461, 393–398 (2009). Article  CAS  Google Scholar  * Lacombe, S. _et al._ Interfamily transfer of a plant pattern-recognition

receptor confers broad-spectrum bacterial resistance. _Nature Biotechnol._ 28, 365–369 (2010). Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS We thank Paul Birch for

providing _P. infestans_ strains 88069td and EC1_DC2005, Gert van Arkel, Nic Boerboom, Nicolas Champouret, Luigi Faino, Marjan Bergervoet and Alireza Salami for technical assistance in ELR

cloning and transformation, Rients Niks, Jack Vossen and Matthieu Joosten for helpful discussions and supervision, Sebastian Schornack for excellent help with microscopy, and Cyril Zipfel

for useful suggestions. This work was supported by a NWO-VIDI grant 12378 (V.G.A.A.V.), the Wageningen University Fund (WUF) (J.D.), the China Scholarship Council Program for Graduate

Students (J.D.), Avebe (E.V., G.B., V.G.A.A.V.), the Gatsby Charitable Foundation (A.C.G., S.K.), the European Research Council (ERC) (A.C.G., S.K.) and the Biotechnology and Biological

Sciences Research Council (BBSRC) (A.C.G., S.K.). AUTHOR INFORMATION Author notes * Juan Du & Edwin A. G. van der Vossen Present address: †Present addresses: Key Laboratory of

Horticultural Plant Biology, Huazhong Agricultural University, Wuhan, Hubei 430070, China (J.D.); Keygene N.V., PO Box 216, Wageningen 6700 AE, The Netherlands (E.A.G.v.d.V.), AUTHORS AND

AFFILIATIONS * Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands Juan Du, Estelle Verzaux, Gerard

Bijsterbosch, Edwin A. G. van der Vossen, Evert Jacobsen, Richard G. F. Visser & Vivianne G. A. A. Vleeshouwers * Key Laboratory of Horticultural Plant Biology, Ministry of Education

National Center for Vegetable Improvement (Central China), Juan Du & Conghua Xie * Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University,

Wuhan, 430070, Hubei, China Juan Du & Conghua Xie * The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Norwich, UK Angela Chaparro-Garcia, Ji Zhou, Silke Robatzek & Sophien

Kamoun * Biometris, PO Box 100, 6700 AC, Wageningen, The Netherlands L. C. Paul Keizer * Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The

Netherlands Thomas W. H. Liebrand & Francine Govers Authors * Juan Du View author publications You can also search for this author inPubMed Google Scholar * Estelle Verzaux View author

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inPubMed Google Scholar * Ji Zhou View author publications You can also search for this author inPubMed Google Scholar * Thomas W. H. Liebrand View author publications You can also search

for this author inPubMed Google Scholar * Conghua Xie View author publications You can also search for this author inPubMed Google Scholar * Francine Govers View author publications You can

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inPubMed Google Scholar * Vivianne G. A. A. Vleeshouwers View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS J.D., E.V., A.C.G., G.B., L.C.P.,

J.Z. and V.G.A.A.V. performed experiments and analysed data; T.W.H.L. and F.G. contributed constructs; J.D., E.V., A.C.G., E.A.G.v.d.V., C.X., J.Z., S.R., S.K. and V.G.A.A.V. designed

experiments; E.A.G.v.d.V., C.X., S.R., S.K., V.G.A.A.V., E.J. and R.G.F.V. supervised; J.D., E.V., A.C.G., S.K. and V.G.A.A.V. wrote the manuscript. CORRESPONDING AUTHOR Correspondence to

Vivianne G. A. A. Vleeshouwers. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION (PDF 2584

KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Du, J., Verzaux, E., Chaparro-Garcia, A. _et al._ Elicitin recognition confers enhanced resistance to

_Phytophthora infestans_ in potato. _Nature Plants_ 1, 15034 (2015). https://doi.org/10.1038/nplants.2015.34 Download citation * Received: 28 July 2014 * Accepted: 26 February 2015 *

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