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Access through your institution Buy or subscribe Brown _et al_. used a principle called second harmonic generation (SHG) — an intrinsic fluorescent signal that can be detected without the
addition of dyes or other reagents — to obtain high-resolution three-dimensional images of fibrillar collagen _in vivo_. SHG imaging allowed the authors to view and measure the density of
fibrillar structures in different tumour types in mice (image shows SHG signal of collagen in red and cancer cell nuclei in green). The SHG signal was found to vary among tumour types and
was correlated with collagen content, as confirmed by other methods such as immunohistochemical staining of tissue sections. When the authors applied collagenase enzymes to mouse melanomas
and imaged them _in vivo_, the SHG signal gradually faded, indicating the breakdown of the collagen matrix. Furthermore, as the SHG signal decreased, the diffusion co-efficient of a labelled
molecular probe increased. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print
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local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES ORIGINAL
RESEARCH PAPER * Brown, E. et al. Dynamic imaging of collagen and its modulation in tumours _in vivo_, using second harmonic generation. _Nature Med._ 9, 796–801 (2003) Article CAS PubMed
Google Scholar FURTHER READING * Jain, R. K., Munn, L. I. & Fukumura, D. Dissecting tumour pathology using intravital microscopy. _Nature Rev. Cancer_ 2, 266–276 (2003). Article
Google Scholar Download references Authors * Kristine Novak View author publications You can also search for this author inPubMed Google Scholar RELATED LINKS RELATED LINKS WEB SITE Rakesh
Jain's lab RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Novak, K. Measuring the matrix. _Nat Rev Cancer_ 3, 394 (2003).
https://doi.org/10.1038/nrc1116 Download citation * Issue Date: 01 June 2003 * DOI: https://doi.org/10.1038/nrc1116 SHARE THIS ARTICLE Anyone you share the following link with will be able
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