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Access through your institution Buy or subscribe The GENSAT project exploits the library of bacterial artificial chromosomes (BACs) associated with the mouse genome sequence. Modified BACs
are produced in which a reporter sequence that encodes an enhanced green fluorescent protein (EGFP) is substituted for the coding region of a gene of interest. Transgenic mouse lines are
then generated that carry the construct, which allows reporter-gene expression to be systematically analysed during development. The resulting data, which have been verified through mRNA _in
situ_ hybridization and immunohistochemistry, have been integrated and analysed to create a digital atlas of the mouse brain that can be viewed online and will be updated whenever new data
are generated. This innovative project is allowing gene expression to be correlated with cell type and the elaborate specializations of morphologically complex cells, such as neurons, to be
visualized. Furthermore, small groups of cells that express a particular gene can be identified and their development followed over time. This is a preview of subscription content, access
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Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES ORIGINAL RESEARCH PAPER * Gong, S. et al. A gene expression atlas of the central
nervous system based on bacterial artificial chromosomes. _Nature_ 425, 917–925 (2003) Article CAS Google Scholar FURTHER READING * Heintz, N. BAC to the future: the use of BAC transgenic
mice for neuroscience research. _Nature Rev. Neurosci._ 3, 861–870 (2001) Article Google Scholar Download references Authors * Victoria Kitchener View author publications You can also
search for this author inPubMed Google Scholar RELATED LINKS RELATED LINKS WEB SITE GENSAT BAC Transgenic Project RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS
ARTICLE Kitchener, V. Mapping the mouse mind. _Nat Rev Genet_ 4, 931 (2003). https://doi.org/10.1038/nrg1235 Download citation * Issue Date: 01 December 2003 * DOI:
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