Play all audios:
Access through your institution Buy or subscribe The authors introduced into fruit fly DL1 cells a three-exon minigene derived from the laccase2 gene, which is known to produce circRNA.
Depletion of several spliceosome proteins largely eliminated the expression of the minigene linear mRNA. By contrast, expression of the minigene circRNA in these cells was only slightly
decreased or even increased. Importantly, the splicing patterns of the endogenous laccase2 gene and of two other circRNA-producing genes were similarly affected. Furthermore, the depletion
of 25 other core spliceosome proteins resulted in increase in circRNA expression from various endogenous genes. These data suggested that the relative efficiency of back-splicing compared
with canonical splicing increases when the quantity of spliceosome proteins is limited. > failure to terminate transcription can result in circRNA production > from downstream genes
This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Access Nature and 54 other Nature Portfolio journals Get Nature+, our
best-value online-access subscription $29.99 / 30 days cancel any time Learn more Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue
Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL
ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Liang, D. et al. The output of protein-coding genes shifts to
circular RNAs when the pre-mRNA processing machinery is limiting. _Mol. Cell_ http://dx.doi.org/10.1016/j.molcel.2017.10.034 (2017) Download references Authors * Eytan Zlotorynski View
author publications You can also search for this author inPubMed Google Scholar RELATED LINKS RELATED LINKS RELATED LINKS IN NATURE RESEARCH Chen, L. L. The biogenesis and emerging roles of
circular RNAs. _Nat. Rev. Mol. Cell Biol._ 17, 205–211 (2016) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Zlotorynski, E. Going in circles. _Nat Rev
Mol Cell Biol_ 19, 3 (2018). https://doi.org/10.1038/nrm.2017.128 Download citation * Published: 06 December 2017 * Issue Date: January 2018 * DOI: https://doi.org/10.1038/nrm.2017.128 SHARE
THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to
clipboard Provided by the Springer Nature SharedIt content-sharing initiative