Play all audios:
Access through your institution Buy or subscribe PDZ domains are protein–protein interaction motifs that are involved in a range of cellular processes. Way and colleagues found that the
highly conserved portion of F11 contains motifs that are characteristic of PDZ domains, and structural predictions suggested that it would adopt a PDZ-like fold. Interestingly, vaccinia
virus with mutated F11 PDZ motifs formed fewer actin tails (which enhance viral spread), showed reduced cell–cell spread and released fewer infectious virions. These effects are indicative
of failed RHOA inhibition, which suggests that the F11 PDZ domain regulates RHOA signalling. Like other GTPases, RHO proteins are regulated by RHO guanine exchange factors (RHOGEFs; which
activate them) and RHO GTPase-activating proteins (RHOGAPs; which inactivate them), both of which are known to have PDZ-binding motifs (PBMs). Through an _in vitro_ pull-down assay, the
authors identified two RHOGAPs, β-chimaerin and myosin IXa, that interacted with the F11 PDZ domain. This is a preview of subscription content, access via your institution ACCESS OPTIONS
Access through your institution Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on
SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about
institutional subscriptions * Read our FAQs * Contact customer support CHANGE HISTORY * _ 20 AUGUST 2013 In the original article, the first author's name (Handa, Y.) was incorrect in
the cited reference; this has now been corrected online. We apologize for any confusion caused. _ REFERENCES * Handa, Y., Durkin, C. H., Dodding, M. P. & Way, M. Vaccinia virus F11
promotes viral spread by acting as a PDZ-containing scaffolding protein to bind myosin-9A and inhibit RhoA signalling. _Cell Host Microbe_ http://dx.doi.org/10.1016/j.chom.2013.06.006 (2013)
Download references Authors * Rachel David View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS
ARTICLE CITE THIS ARTICLE David, R. Propelling vaccinia virus to the neighbours. _Nat Rev Microbiol_ 11, 595 (2013). https://doi.org/10.1038/nrmicro3091 Download citation * Published: 22
July 2013 * Issue Date: September 2013 * DOI: https://doi.org/10.1038/nrmicro3091 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable
link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative