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Access through your institution Buy or subscribe CaM interacts with calcium channels to carry out two functions: it mediates calcium-dependent inactivation of the channels, and it transduces
the signal from calcium entry through the channels into various intracellular signalling pathways. Mori _et al_. fused CaM molecules to L-type calcium channels to investigate the
stoichiometry of these processes. In the absence of calcium, CaM molecules pre-associate with L-type calcium channels. When calcium subsequently enters through the channel and interacts with
CaM, the channel is inactivated. The authors used polyglycine linkers to fuse an inactive, mutant CaM (CaMMUT) to each L-type calcium channel, on the principle that the CaMMUT would act as
a dominant-negative and prevent wild-type CaM from associating with the channel. Channel–CaMMUT chimaeras did not undergo calcium-dependent inactivation, but channels fused to a wild-type
CaM did, indicating that the fused CaM is important for inactivation. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution
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Contact customer support REFERENCES ORIGINAL RESEARCH PAPER * Mori. M. X. et al. Functional stoichiometry and local enrichment of calmodulin interacting with Ca2+ channels. _Science_ 304,
432–435 (2004) Article CAS Google Scholar FURTHER READING * Budde, T. et al. Calcium-dependent inactivation of neuronal calcium channels. _Nature Rev. Neurosci._ 3, 873–883 (2002) Article
CAS Google Scholar Download references Authors * Rachel Jones View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and
permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Jones, R. Counting CaMs at calcium channels. _Nat Rev Neurosci_ 5, 432 (2004). https://doi.org/10.1038/nrn1417 Download citation * Issue
Date: 01 June 2004 * DOI: https://doi.org/10.1038/nrn1417 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable
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